Development of a lateral flow assay for the detection of Salmonella based on antibodies against peptides of the effect or protein SipC of the type III secretion system of Salmonella spp.

Abstract

KEYWORDS Salmonella SipCEffectors protein translocation Immunoassay lateral flow assay ABSTRACTPresent study was the first promising steps in developing a diagnostic procedure for detecting a Salmonella infection or contamination. The areas of application are human medicine, veterinary medicine and various industrial sectors. The development was based on the finding that high antigen areas are formed in the effectors protein SipC. These areas are used to generate antibodies against the synthetically produced peptides from 15-17 amino acids. By using a non-competitive lateral flow assay (FLA) developed under laboratory conditions, a Salmonella contamination could be successfully detected by the SipC protein released into the culture medium.