Development of a Long-Range Surface-Enhanced Raman Spectroscopy Ruler

Singh, Anant Kumar; Khan, Sadia Afrin; Fan, Zhen; Demeritte, Teresa; Senapati, Dulal; Kanchanapally, Rajashekhar; Ray, Paresh Chandra

doi:10.1021/ja301921k

Cited by 80 publications

(186 citation statements)

References 57 publications

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“…Gold nanoparticle synthesis was achieved through our recently reported method, with additional modifications 9,18–20,30 . Briefly, AuNPs (10–15 nm in diameter) were synthesized by using 0.01% chloroauric acid and 1% trisodium citrate dehydrate (weight/volume).…”

Section: Methodsmentioning

confidence: 99%

Bioconjugated Gold Nanoparticle Based SERS Probe for Ultrasensitive Identification of Mosquito-Borne Viruses Using Raman Fingerprinting

et al. 2015

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Dengue virus (DENV) and West Nile virus (WNV) are two well-documented mosquito-borne flaviviruses that cause significant health problems worldwide. Driven by this need, we have developed a bio-conjugated gold nanoparticle (AuNP)-based surface enhanced Raman spectroscopy (SERS) probe for the detection of both DENV and WNV. Reported data demonstrate anti-flavivirus 4G2 antibody conjugated gold nanoparticle (GNP) SERS probe can be used as a Raman fingerprint for the ultrasensitive detection of DENV and WNV selectively. Experimental data show that due to the plasmon coupling in nano-assembly, antibody conjugated GNP- based SERS is able to detect as low as 10 plaque-forming units (PFU)/ml of DENV-2 and WNV, which is comparable with the sensitivity of quantitative PCR-based assays. Selectivity of our probe was demonstrated using another mosquito-borne chikungunya virus (CHIKV) as a negative control. Experimental data demonstrate a huge enhancement of SERS intensity is mainly due to the strong electric field enhancement, which has been confirmed by the finite-difference time-domain (FDTD) simulation. Reported FDTD simulation data indicate the SERS enhancement factor can be more than 104 times, due to the assembled structure. Reported results suggest that bio-conjugated AuNP-4G2 based SERS probes have great potential to be used to screen viral particles in clinical and research-based laboratories.

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Section: Methodsmentioning

confidence: 99%

Bioconjugated Gold Nanoparticle Based SERS Probe for Ultrasensitive Identification of Mosquito-Borne Viruses Using Raman Fingerprinting

et al. 2015

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“…Distance dependent SERS studies necessitate the presence of spacer between the silver surface and the analyte molecules. Many such spacers have been employed in the past, such as double stranded DNA, 34 thio-methylene spacers, 35 polymer coating (polyethylene glycol or 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 7 polystyrenesulfonate/polydiallydimethylammonium-hydroxide), 36,37 silica or alumina coating etc. 26,29 Each method has its own limitations.…”

Section: Toc Graphicsmentioning

confidence: 99%

How Far Can We Probe by SERS?

2015

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Surface-enhanced Raman spectroscopy (SERS) has gained paramount importance in the recent past due to its widespread applications in biodetection, monitoring chemical reactions, small molecule protein interactions, etc. It is believed that SERS is a distance-dependent phenomenon and is effective within 1 nm from the nanoparticle surface. In this work, we have investigated this distance dependence of SERS as a function of nanoparticle size. Earlier attempts have made use of flexible separators, like DNA and chemical molecules, between nanoparticle and analyte to vary the distance. We have used silica coating to vary the distance, without ambiguity, of the analyte from the silver nanoparticle surface. Our results suggest that SERS is observed up to a distance of 1 nm for 20 nm silver nanoparticles juxtaposed to 5 nm in the case of 90 nm silver nanoparticles. This is due to large scattering cross sections and increased radiative damping in the case of the larger nanoparticles. This study gives direct correlation between the size of nanoparticles and distance probed through SERS which would aid in designing nanoparticle system for various applications and analytes in the future.

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“…Recently it has been reported that more the DNA molecule bonds to the surface, the lesser is the probability of bending 24 . Spherical gold nanoparticles of around 40 nm size were synthesized by using gold chloride (HAuCl 4 , 3H 2 O) and sodium citrate, using citrate reduction method as we have reported before 11,13,14 . Details of the synthesis and characterizations have been reported in the experimental section.…”

Section: Resultsmentioning

confidence: 99%

“…Gold nanoparticles around nm in size were synthesized by using 0.01 wt.% chlorauric acid (HAuCl 4 ) and 1 wt.% trisodium citrate dehydrate, as reported by our groups before 11,13,14 . After that spherical gold nanoparticles were characterized using TEM, as reported in Figure 6A, which indicates that the size is about 40 nm.…”

Section: Methodsmentioning

confidence: 99%

“…To find out the validity of rigid-rod approximation we have used dynamic light scattering (DLS) measurement using a Malvern Zetasizer Nano instrument. We have reported before that DLS can be used to measure the distance between GNP and dye, when they are separated by –ds DNA/RNA 11,14 . Table 1 clearly shows that that the DLS measurement data are quite close to our estimated distances using rigid-rod approximation.…”

Section: Methodsmentioning

confidence: 99%

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Designing a multicolor long range nanoscopic ruler for the imaging of heterogeneous tumor cells

et al. 2016

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Tumor heterogeneity is one of the biggest challenges in cancer treatment and diagnosis. Multicolor optical ruler is essential to address heterogeneous tumor cells complexity. Driven by the need, current article reports the design of multicolor long range nanoscopic ruler for screening tumor heterogeneity by accurately identifying epithelial cells and cancer stem cells (CSCs) simultaneously. Nanoscopic surface energy transfer (NSET) ruler has been developed using blue fluorescence polymer dots (PDs) and red fluorescence gold cluster dots (GCDs) as multicolor fluorescence donor and plasmonic gold nanoparticle (GNP) acts as an excellent acceptor. Reported experimental results demonstrated that the multicolor nanoscopic ruler’s working window is above 35 nm distances, which is more than three times farther than that of Förster resonance energy transfer (FRET) distance limit. Theoretical modeling using Förster dipole–dipole coupling and dipole to nanoparticle surface energy transfer have been used to discuss the possible mechanism for multicolor nanoscopic ruler’s long-range capability. Using RNA aptamer that are specific for the target cancer cells, experimental data demonstrate that nanoscopic ruler can be used for screening epithelial and CSCs simultaneously from whole blood sample with 10 Cells/mL detection capability. Experimental data show that nanoscopic ruler can distinguish targeted cells from non-targeted cells.

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Development of a Long-Range Surface-Enhanced Raman Spectroscopy Ruler

Cited by 80 publications

References 57 publications

Bioconjugated Gold Nanoparticle Based SERS Probe for Ultrasensitive Identification of Mosquito-Borne Viruses Using Raman Fingerprinting

Bioconjugated Gold Nanoparticle Based SERS Probe for Ultrasensitive Identification of Mosquito-Borne Viruses Using Raman Fingerprinting

How Far Can We Probe by SERS?

Designing a multicolor long range nanoscopic ruler for the imaging of heterogeneous tumor cells

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