Development of a Magnetic Nanoparticle-Based Artificial Cleavage Reagent for Site-Selective Cleavage of Single-Stranded DNA

Abstract

A novel type of magnetic nanoparticle-based artificial cleavage reagent for single-stranded DNA was designed and synthesized by binding a molecular scissor on the surface of magnetic nanoparticles. This cleavage reagent not only was effective but also presented satisfying cleavage specificity. Most importantly, the magnetic nanoparticles greatly facilitate the isolation of cleaved products.

Au@organosilica multifunctional nanoparticles for the multimodal imaging

“…15 More recently, our group have successfully shortened the time for coating the initial silica shell of 4-5 nm from sodium silicate to about one hour by elevating the reaction temperature from room temperature to 90 C. 16 However, these TEOS or sodium silicate-based SiO 2 coating methods provide only silanol groups on the surface, which require further modification to introduce some specific functional groups, such as -SH, -NH 2 , -CHO for the subsequent binding with fluorescence molecules or biomolecules. [17][18][19] In a typical process, the prepared SiO 2 should be silanized with 3-mercaptopropyltrimethoxysilane (MPTMS), APS, or N 1 -[3-(trimethoxysilyl)-propyl]diethylenetriamine firstly. Then, the functionalized silica nanoparticles are conjugated with disulfide-modified oligonucleotides via the thiol/disulfide exchange reaction or with enzymes or antibodies via crosslinking between amines using glutaraldehyde.…”