“…The water supply is the source of life, and the presence of these poisons has a negative impact on the environment, putting the health of both humans and animals in jeopardy. Numerous produced goods, including textiles, pharmaceuticals, food, cosmetics, and agro‐allied sectors, make extensive use of organic dyes via their widespread use 5,6 . The reduced form of methylene orange (MO), which is an anionic azo dye that is frequently used in the textile, pharmaceutical, paper, leathers, printing, cosmetics, and food sectors, has the potential to be mutagenic.…”