Development of a Mesoamerican intra-genepool genetic map for quantitative trait loci detection in a drought tolerant × susceptible common bean (Phaseolus vulgaris L.) cross

Abstract

Drought is a major constraint to common bean (Phaseolus vulgaris L.) production, especially in developing countries where irrigation for the crop is infrequent. The Mesoamerican genepool is the most widely grown subdivision of common beans that include small red, small cream and black seeded varieties. The objective of this study was to develop a reliable genetic map for a Mesoamerican × Mesoamerican drought tolerant × susceptible cross and to use this map to analyze the inheritance of yield traits under drought and fully irrigated conditions over 3 years of experiments. The source of drought tolerance used in the cross was the cream-seeded advanced line BAT477 crossed with the small red variety DOR364 and the population was made up of recombinant inbred lines in the F5 generation. Quantitative trait loci were detected by composite interval mapping for the traits of overall seed yield, yield per day, 100 seed weight, days to flowering and days to maturity for each field environment consisting of two treatments (irrigated and rainfed) and lattice design experiments with three repetitions for a total of six environments. The genetic map based on amplified fragment length polymorphism and random amplified polymorphic DNA markers was anchored with 60 simple sequence repeat (SSR) markers and had a total map length of 1,087.5 cM across 11 linkage groups covering the whole common bean genome with saturation of one marker every 5.9 cM. Gaps for the genetic map existed on linkage groups b03, b09 and b11 but overall there were only nine gaps larger than 15 cM. All traits were inherited quantitatively, with the greatest number for seed weight followed by yield per day, yield per se, days to flowering and days to maturity. The relevance of these results for breeding common beans is discussed in particular in the light of crop improvement for drought tolerance in the Mesoamerican genepool.

Análise da capacidade combinatória em feijoeiro comum submetido ao déficit hídrico

“…In this context, the increased population growth combined with climate change and the need for food production on a large scale will require the cultivation of plants more adapted to stress conditions (McClean et al, 2011). Upon the intensification of a biotic stresses, several research groups on the genetic improvement of beans have implemented multidisciplinary programs to analyze a biotic stresses aimed at identifying drought tolerant genotypes, exploring their genetic variability, such as early maturity, deep rooting for greater absorption of water, physiological adaptation through stomatal conductance, along with satisfactory grain yield in stressed crops (Blair et al, 2012). Singh et al (2001) registered the line SEA 5 as a drought tolerant cultivar, derived from interracial crosses between the races Mesoamerican and Durango, and one of the parents originating the line was the cultivar BAT 477, also described by the authors as drought tolerant.…”

Morphoagronomic characterization and genetic diversity of a common bean RIL mapping population derived from the cross Rudá x AND 277

“…Other investigators also have observed significant differences in the traits DFL, DH, ARQ, PROD, H, J, and M100 in common bean. Blair et al (2012) reported significant effects for DFL, DH, PROD, and M100, in 113 RILs derived from parents belonging to the same Mesoamerican gene pool. Faleiro et al (2003) investigated 154 RILs derived from a cross between Ouro Negro and Rudá (both from the Mesoamerican gene pool) and found significant effects for the traits DFL, DH, and M100.…”

“…Currently, however, seeds of these RILs are not readily available. Another mapping population was developed by Blair et al (2012) and comprises 113 RILs derived from a cross between DOR 364 and BAT 447; both parents belong to the Mesoamerican gene pool. The small size of this population is a limitation; moreover, the availability of seeds for exchange and use by different teams is low.…”

Functional Genomics of Biotic and Abiotic Stresses in Phaseolus vulgaris

“…Drought field trials performed with the RIL population of the crossing BAT 477 × DOR 364, previously referred to the greenhouse experiment, showed significant variability across four locations evaluated [104]. A QTL analysis associating the field traits to a previous set of molecular markers disposed in a linkage map [106] showed significant QTL-environment interactions. Therefore, determining if one cultivar is tolerant to drought does not necessarily mean it will respond well to all environments, in a sense that it must be tested in multiple environments to check for its performance.…”