Development of a method for identifying elevated vitellogenin gene expression in the Sydney Rock Oyster (<i>Saccostrea glomerata</i>) as an indicator of endocrine disruption on the Sunshine Coast

Anderson, Kling L.; Burnell, F. J.; Roiko, Anne; Andrew, M.N.; O’Connor, Wayne A.; Elizur, Abigail

doi:10.1111/j.1442-8903.2010.00534.x

Cited by 9 publications

(3 citation statements)

References 8 publications

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“…Ultimately these molecular interactions and cellular responses may be causing 6.) male mussel gonadal cells to differentiate into female reproductive tissue resulting in feminization of male mussels. ,, The changes in reproductive transcripts and gonadal tissue may also be causing 7.) reduced fertilization and production of viable eggs and 8.)…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic and Network Analyses Reveal Mechanistic-Based Biomarkers of Endocrine Disruption in the Marine Mussel, Mytilus edulis

Blalock

Robinson

Loguinov

et al. 2018

Environ. Sci. Technol.

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Transcriptomics, high-throughput assays, and adverse outcome pathways (AOP) are promising approaches applied to toxicity monitoring in the 21st century, but development of these methods is challenging for nonmodel organisms and emerging contaminants. For example, Endocrine Disrupting Compounds (EDCs) may cause reproductive impairments and feminization of male bivalves; however, the mechanism linked to this adverse outcome is unknown. To develop mechanism-based biomarkers that may be linked through an AOP, we exposed Mytilus edulis to 17-alpha-ethinylestradiol (5 and 50 ng/L) and 4-nonylphenol (1 and 100 μg/L) for 32 and 39 days. When mussels were exposed to these EDCs, we found elevated female specific transcripts and significant female-skewed sex ratios using a RT-qPCR assay. We performed gene expression analysis on digestive gland tissue using an M. edulis microarray and through network and targeted analyses identified the nongenomic estrogen signaling pathway and steroidogenesis pathway as the likely mechanisms of action for a putative AOP. We also identified several homologues to genes within the vertebrate steroidogenesis pathway including the cholesterol side chain cleavage complex. From this AOP, we designed the Coastal Biosensor for Endocrine Disruption (C-BED) assay which was confirmed in the laboratory and tested in the field.

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Section: Resultsmentioning

confidence: 99%

Transcriptomic and Network Analyses Reveal Mechanistic-Based Biomarkers of Endocrine Disruption in the Marine Mussel, Mytilus edulis

Blalock

Robinson

Loguinov

et al. 2018

Environ. Sci. Technol.

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“…) and Saccostrea glomerata Gould 1850 (Anderson et al. ). About 20–35% identity was found between the deduced amino acid sequences of the VTGs of some of these species, and the similarity among the sequences was limited to the N‐terminal region, when compared with other oviparous vertebrates and invertebrates (Osada et al.…”

mentioning

confidence: 99%

Cloning and expression analysis of the vitellogenin gene in the scallop Chlamys farreri and the effects of estradiol‐17β on its synthesis

Qin

Sun

et al. 2012

Invertebrate Biology

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Vitellogenin (VTG), the precursor of yolk protein, plays an important role during vitellogenesis and oogenesis. However, little is known about the vitellogenin gene in marine bivalves. We cloned the full-length cDNA of Chlamys farreri vitellogenin (Cf-vtg) using a homologous cloning strategy and rapid amplification of cDNA ends. The full-length cDNA consisted of 7604 nucleotides with an open reading frame encoding 2296 amino acid residues. The deduced amino acid sequence shared high similarity with the VTG of other species, particularly in the N-terminal region. We analyzed the spatiotemporal expression of Cf-vtg transcripts by semiquantitative and quantitative real-time PCR. Cf-vtg was primarily expressed in the ovary, and levels were 1.917±0.035 (mean±S.E.) and 2.570±0.252 times higher in growing and mature stages, respectively, than in the proliferative stage. There was negligible expression at the resting stage. Interestingly, we observed weak expression in the female hepatopancreas. The level was 4.50±0.90% (mean±S.E.) that in the ovary during the proliferative stage. We did not detect any expression in testis or in other tissues. Using in situ hybridization, Cf-vtg transcripts were localized to the follicle cells surrounding the oocytes in the ovary. Moreover, synthesis of Cf-vtg transcripts in the ovary increased when estradiol-17b was injected in vivo into the gonads of early growing stage. We conclude that VTG synthesis is heterosynthetic in C. farreri. Also, we hypothesize that Cf-VTG synthesis occurs primarily in the follicle cells and is mediated by estradiol-17b.

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“…Expression of Vtg mRNA is mainly assessed by reverse transcription RT-PCR and quantitative RT-PCR (qRT-PCR). Because of its quantitative nature, qRT-PCR has been extensively used in a number of species such as mussels Elliptio complanata (Gagné et al, 2005), Mytilus edulis (Puinean and Rotchell, 2006;Ciocan et al, 2010), scallops, Chlamys farreri (Zhang et al, 2012) and Sydney rock oysters, Saccostrea glomerata (Anderson et al, 2010;Andrew-Priestley et al, 2012;Tran et al, 2016a, b).…”

Section: Methods For the Determination Of Vitellogenin In Molluscsmentioning

confidence: 99%

The utility of vitellogenin as a biomarker of estrogenic endocrine disrupting chemicals in molluscs

Tran

Islam

et al. 2019

Environmental Pollution

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Estrogenic endocrine disrupting chemicals (EDCs) are natural hormones, synthetic compounds or industrial chemicals that mimic estrogens due to their structural similarity with estrogen's functional moieties. They typically enter aquatic environments through wastewater treatment plant effluents or runoff from intensive livestock operations. Globally, most natural and synthetic estrogens in receiving aquatic environments are in the low ng/L range, while industrial chemicals (such as bisphenol A, nonylphenol and octylphenol) are present in the µg to low mg/L range. These environmental concentrations often exceed laboratory-based predicted no effect concentrations (PNECs) and have been evidenced to cause negative reproductive impacts on resident aquatic biota. In vertebrates, such as fish, a well-established indicator of estrogen-mediated endocrine disruption is overexpression of the egg yolk protein precursor vitellogenin (Vtg) in males. Although the vertebrate Vtg has high sensitivity and specificity to estrogens, and the molecular basis of its estrogen inducibility has been well studied, there is growing ethical concern over the use of vertebrate animals for contaminant monitoring. The potential utility of the invertebrate Vtg as a biomonitor for environmental estrogens has therefore gained increasing attention. Here we review evidence providing support that the molluscan Vtg holds promise as an invertebrate biomarker for exposure to estrogens. Unlike vertebrates, estrogen signalling in invertebrates remains largely unclarified

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Development of a method for identifying elevated vitellogenin gene expression in the Sydney Rock Oyster (Saccostrea glomerata) as an indicator of endocrine disruption on the Sunshine Coast

Cited by 9 publications

References 8 publications

Transcriptomic and Network Analyses Reveal Mechanistic-Based Biomarkers of Endocrine Disruption in the Marine Mussel, Mytilus edulis

Transcriptomic and Network Analyses Reveal Mechanistic-Based Biomarkers of Endocrine Disruption in the Marine Mussel, Mytilus edulis

Cloning and expression analysis of the vitellogenin gene in the scallop Chlamys farreri and the effects of estradiol‐17β on its synthesis

The utility of vitellogenin as a biomarker of estrogenic endocrine disrupting chemicals in molluscs

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