A diverse range of therapeutic agents, such as antibiotics, anti-cancer drugs, and wound healing materials, have been physically or chemically formulated on the surface of electrospun nanofibers for achieving controlled topical release within a specific time. In this work, a nanofiber network of a bioactive glass/carboxymethyl cellulose/βcyclodextrin (BAG/CMC/β-CD) was fabricated through electrospinning. Various analytical techniques, including X-ray diffraction analysis (XRD), Fourier-transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), the Brunauer-Emmett-Teller (BET) specific surface area, Field emission scanning electron microscopy (FESEM), Energy dispersive x-ray spectroscopy (EDX), and Transmission electron microscopy (TEM) were used to characterize the synthesized samples. The BAG/CMC/β-CD nanofibers with a diameter of 10 nm were intended to be used for flutamide drug delivery in a simulated intestinal environment. The loading and release effects of flutamide at different times under in vitro conditions were investigated by UV-Vis Spectroscopy. The findings suggested that BAG/CMC/β-CD NF had a high drug loading efficiency of 99.65% and a sustained drug release capacity without an initial burst release, making them a promising candidate for flutamide drug delivery.