Development of a microarray-based assay for efficient testing of new HSP70/DnaK inhibitors

Study on the mechanism and therapeutic effect of Antelope horn treatment on cerebral ischemia reperfusion injury of mouse

“…Ischemic pre-conditioning and post-conditioning can induce increased expression of Hsp 27, which can protect the tissue from ischemia reperfusion injury, thus constituted an endogenous protective mechanism. Studies have reported that Hsp 27 can protect cerebral ischemia reperfusion injury (Sadowska-Krępa et al, 2017), increased Hsp 27 could reduce azithromycin-induced cardiac cell death (Kumar et al, 2016), and Hsp 27 can alleviate cerebral ischemia reperfusion injury (Mohammadi-Ostad-Kalayeh et al, 2017). This research found that, compared with the ischemic control group, Hsp 27 and Hsp 27 levels increased significantly after medication, suggesting that the protective effect of Antelope horn on cerebral ischemia reperfusion is correlated with the elevation of Hsp 27 and Hsp 70.…”

A Network Pharmacology‐Based Approach to Investigate the Novel TCM Formula against Huntington’s Disease and Validated by Support Vector Machine Model

“…Thirty-one known ligands [33] for CK2 protein with IC50 information constructed the predicted model by SVM and MLR algorithm. Thirty-five ligands [34] for meHSP70 protein with IC50 information conducted the predicted model as well. The STK3 and LATS1 protein constructed the predicted models through the reported TCM formula, and the active ingredients would be gathered in our TCM database.…”

Adhesiomics - Testing Protein-Protein Interaction of Cell Adhesion Proteins on Microarrays

“…The plasmids containing the target genes of interest were transformed into competent E.coli cells BL21 (DE3). The plasmid of pET15.1 for human Hsp90a [3] and pETSUMO was utilized for Hsp70 [7],Hsf1and L1CAM (commercial) while the plasmids pEXP5-CT containing human TP53(commercial) or human PP5, respectively. Afterwards, E. coli cells were cultivated and expressed the protein.…”

“…Protein microarray technology was used recently for targetoriented screening of chemically synthesized compound libraries or isolated natural compounds on Heat-Shock Proteins (Hsp), since Hsps together with the accompanied stress proteome are relevant targets in cancer and pathogenic organism [1][2][3][4][5][6][7][8][9]. An extended application of this method showed recently that the microarray technology enables also studies of protein-protein interaction to enable a broader application range [4,7]. The basic prerequisite for the formation of multicellular organisms is the ability of a single cell to communicate with neighboring cells.…”