Development of a Microchip-Based Bioassay System Using Cultured Cells

Goto, Makiko; Sato, Katsuaki; Murakami, Atsushi; Tokeshi, Manabu; Kitamori, Takehiko

doi:10.1021/ac040165g

Cited by 98 publications

(71 citation statements)

References 29 publications

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“…This flow rate is compared with the flow rates used in integrated chemical systems for several practical applications on glass microchips [17,41,42] In these reports, the typical flow rates used for cell cultures in microchips are on the order of 0.1 µL/min; that used for analyses and syntheses in microchips is approximately 1 µL/min. Thus, the pump demonstrated can be used for some of these applications.…”

Section: Dependency On Frequencymentioning

confidence: 99%

A Peristaltic Pump Integrated on a 100% Glass Microchip Using Computer Controlled Piezoelectric Actuators

Tanaka¹

2014

Micromachines

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Lab-on-a-chip technology is promising for the miniaturization of chemistry, biochemistry, and/or biology researchers looking to exploit the advantages of a microspace. To manipulate fluid on a microchip, on-chip pumps are indispensable. To date, there have been several types of on-chip pumps including pneumatic, electroactive, and magnetically driven. However these pumps introduce polymers, metals, and/or silicon to the microchip, and these materials have several disadvantages, including chemical or physical instability, or an inherent optical detection limit. To overcome/avoid these issues, glass has been one of the most commonly utilized materials for the production of multi-purpose integrated chemical systems. However, glass is very rigid, and it is difficult to incorporate pumps onto glass microchips. This paper reports the use of a very flexible, ultra-thin glass sheet (minimum thickness of a few micrometers) to realize a pump installed on an entirely glass-based microchip. The pump is a peristaltic-type, composed of four serial valves sealing a cavity with two penetrate holes using ultra-thin glass sheet. By this pump, an on-chip circulating flow was demonstrated by directly observing fluid flow, visualized via polystyrene tracking particles. The flow rate was proportional to the pumping frequency, with a maximum flow rate of approximately 0.80 μL/min. This on-chip pump could likely be utilized in a wide range of applications which require the stability of a glass microchip.

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Section: Dependency On Frequencymentioning

confidence: 99%

A Peristaltic Pump Integrated on a 100% Glass Microchip Using Computer Controlled Piezoelectric Actuators

Tanaka¹

2014

Micromachines

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“…Delamarche et al first introduced the use of microfluidic channel networks for surface patterning of immunoglobulin in high throughput, parallel immunoassays (44). It should also be noted that microbeads have often been used in microfluidic systems for heterogeneous immunoassays (36,(45)(46)(47)(48) (49). In this study, the porosity of the beads is important since it lowers the fluidic resistance and increases the active surface area, enhancing assay sensitivity.…”

Section: Protein Functionalitymentioning

confidence: 92%

Recent advances in microfluidic technologies for biochemistry and molecular biology

Cho¹,

Kang²,

Choo³

et al. 2011

BMB Reports

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“…They achieved a detection limit of 30 nM for xylenecyanol solution with a sensitivity of 20-100 times that obtained by spectrophotometry. Goto et al [68] developed a microchip system with TLM detection for bioassaying cultured cells. With cell culture, chemical and enzymatic reactions, and detection integrated into a single glass microdevice, nitric oxide release from peritoneal macrophage cells could be monitored and the total assay time reduced from 24 to 4 h. Impressively, the LOD was improved two orders of magnitude over conventional batch methods.…”

Section: Thermal Lens Detectionmentioning

confidence: 99%

Unconventional detection methods for microfluidic devices

2006

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The direction of modern analytical techniques is to push for lower detection limits, improved selectivity and sensitivity, faster analysis time, higher throughput, and more inexpensive analysis systems with ever-decreasing sample volumes. These very ambitious goals are exacerbated by the need to reduce the overall size of the device and the instrumentation -the quest for functional micrototal analysis systems epitomizes this. Microfluidic devices fabricated in glass, and more recently, in a variety of polymers, brings us a step closer to being able to achieve these stringent goals and to realize the economical fabrication of sophisticated instrumentation. However, this places a significant burden on the detection systems associated with microchip-based analysis systems. There is a need for a universal detector that can efficiently detect sample analytes in real time and with minimal sample manipulation steps, such as lengthy labeling protocols. This review highlights the advances in uncommon or less frequently used detection methods associated with microfluidic devices. As a result, the three most common methods -LIF, electrochemical, and mass spectrometric techniques -are omitted in order to focus on the more esoteric detection methods reported in the literature over the last 2 years.

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Development of a Microchip-Based Bioassay System Using Cultured Cells

Cited by 98 publications

References 29 publications

A Peristaltic Pump Integrated on a 100% Glass Microchip Using Computer Controlled Piezoelectric Actuators

A Peristaltic Pump Integrated on a 100% Glass Microchip Using Computer Controlled Piezoelectric Actuators

Recent advances in microfluidic technologies for biochemistry and molecular biology

Unconventional detection methods for microfluidic devices

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