This article analyses vibration transmission on agricultural tractors with the excitation from the road to the driver’s seat. A multi-body model of agricultural tractors created in Adams is presented. The main parts for the investigation of vibration transmission are the tractor body, where the only suspension elements are tyres, the tractor cabin, spring-dampers suspended at the rear and bushings at the front, and the driver seat with its pneumatic spring. A series of measurements were performed, and the model was validated using vertical acceleration values on the tractor body at four different locations. The FTire model (physical FEM-based model) was chosen to describe the behaviour of tyres. The model was created using measured tyre characteristics. Measured characteristics of spring-dampers and front cabin bushings were also implemented. For comfort investigation, ride simulations on ISO 5008 rough roads were performed. The transmission of vibrations in ride simulations was examined. A modal analysis of the linearised model was performed to confirm assumptions of the contribution of suspension elements to overall vibration levels. Finally, three case studies were conducted to better understand the model’s vibration transmission properties.