Development of a model to demonstrate photosensitizer‐mediated viral inactivation in blood

Hc, Neyndorff; Dl, Bartel; Tufaro, Frank; Jg, Levy

doi:10.1046/j.1537-2995.1990.30690333476.x

Cited by 96 publications

(44 citation statements)

References 8 publications

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“…This model was used together with the dye-uptake technique [18] because the cytotoxicity of the materials can be determined by spectrophotometry.…”

Section: Discussionmentioning

confidence: 99%

“…The cytotoxicity of these orthodontic elastics was determined through the dye-uptake technique [18], which is based on the neutral red absorption by living cells. How these chain elastics are usually maintained in the oral cavity for up to 4 weeks, since patients wearing fixed appliances usually visit the orthodontist once a month, the periods of time: 1, 2, 3, 7, and 28 days were evaluated in this study.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of the cytotoxicity of polyurethane and non-latex orthodontic chain elastics

et al. 2012

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Allergy caused by latex proteins has been well documented. Thus, the study of non-latex materials, is necessary. The aim of this study is to evaluate the cytotoxicity of silver-coloured orthodontic chain elastics, of polyurethane and latex-free. Nine chain elastics from different manufactures (3 latex-free and 6 polyurethane) were divided into 9 groups of 10 elastics each: Group UK (Latex-free, 3M Unitek), Group TP (Látex-free, TP Orthodontics), Group AO (Látex-free, American Orthodontics), Group O (Polyurethane, OrthoSource), Group M (Polyurethane, Morelli), Group TD (Polyurethane, Tecnident), Group UD (Polyurethane, Uniden), Group AZ (Polyurethane, Abzil) and Group AK (Polyurethane, Aditek). The cytotoxicity essay was performed using cell cultures (L-929 line cells, mouse fibroblast) that were submitted to the cell viability test with neutral red ("dye-uptake") at 1, 2, 3, 7 and 28 days. Analysis of variance (ANOVA) with multiple comparisons and Tukey's test were employed (p<.05). The results showed no statistically significant differences between Groups UK, TP and AO in all experimental times (p>.05), except between the Groups UK and TP at 28 days (p<.05). There was significant statistically difference (p<.05) between the viability of the cells in the Groups O and TD with the Groups M, AZ, AK at 1 and 2 days. The polyurethane elastics showed lower cell viability in the first 48 hours, with increase on 3rd and 7th day, and viability similar to latex-free elastics at 28 days. It can be concluded that the latex-free chain elastic showed higher cell viability. The OrthoSource and Tecnident trademark showed lower cell viability the initial 48 hours.

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“…This model was used together with the dye-uptake technique [18] because the cytotoxicity of the materials can be determined by spectrophotometry.…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Evaluation of the cytotoxicity of polyurethane and non-latex orthodontic chain elastics

et al. 2012

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“…The cytotoxicity assay was performed by incubating, in triplicate, Vero cell monolayers cultivated in 96-well microplates with two-fold serial dilutions of the extracts for 48 h at 37 o C. Morphological alterations of the treated cells were observed in an inverted optical microscope (Leitz-Germany 633456), and the maximum non-toxic concentrations (MNTC) were determined (Walker et al, 1972). Cellular viability was further evaluated by the neutral red dye-uptake method (Neyndorff et al, 1990). The 50% cytotoxic concentration (CC50) was defined as the dilution that caused a reduction of 50% in the number of viable cells.…”

Section: Cytotoxicitymentioning

confidence: 99%

Antiviral activity of extracts from Brazilian seaweeds against herpes simplex virus

Soares¹,

Robaina²,

Mendes³

et al. 2012

Rev. bras. farmacogn.

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Organic extracts of 36 species of marine algae (sixteen species of Rhodophyta, eight species of Ochrophyta and twelve species of Chlorophyta) from seven locations on the Brazilian coast were evaluated for their anti-HSV-1 and anti-HSV-2 activity resistant to Acyclovir (ACV). Activity tests in crude extracts, followed by the identification of the major compounds present, were performed for all species. The chemical profiles of all crude extracts were obtained by 1 H-NMR and 13 C-NMR spectroscopy. The percentage of extracts with antiviral activity was higher for HSV-1 (86.1%) than for HSV-2 (55.5%). The green algae Ulva fasciata and Codium decorticatum both showed the highest activity (99.9%) against HSV-1, with triacylglycerols and fatty acids as the major components. The red alga Laurencia dendroidea showed good activity against HSV-1 (97.5%) and the halogenated sesquiterpenes obtusol and (-)-elatol were identified as the major components in the extract. Against HSV-2, the green alga Penicillus capitatus (Chlorophyta) and Stypopodium zonale (Ochrophyta) were the most active (96.0 and 95.8%). Atomaric acid, a meroditerpene, was identified as the major secondary metabolite in the S. zonale extract. These results reinforce the role of seaweeds as important sources of compounds with the potential to enter into the pipeline for development of new drugs against herpes simplex.

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“…As an alternative to latex, front the reports of latex allergy [26] and their cytotoxic character in literature [16][17], this study evaluated the cytotoxicity of polyurethane and nonlatex Orthodontic elastomeric ligatures of different manufactures.…”

Section: Discussionmentioning

confidence: 99%

“…The cytotoxicity of these orthodontic elastics was determined through the dye-uptake technique [16], which is based on the neutral red absorption by living cells. How these chain elastics are usually maintained in the oral cavity for up to 4 weeks, since patients wearing fixed appliances usually visit the orthodontist once a month, the periods of time: 1, 2, 3, 7, and 28 days were evaluated in this study.…”

Section: Methodsmentioning

confidence: 99%

Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

et al. 2015

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The aim of this study is to evaluate cytotoxicity between polyurethane and non-latex orthodontic elastomeric ligatures. Seven elastomeric ligatures of black-coloured from different manufactures (3 non-latex, 3 polyurethane and 1 latex) were divided into 7 groups of 10 elastics each: Group UK (nonlatex, 3M Unitek), Group TP (nonlatex, TP Orthodontics), Group AO (nonlatex, American Orthodontics), Group O (Polyurethane, OrthoSource), Group M (Polyurethane, Morelli), Group TD (Polyurethane, Tecnident) and Group TC (latexControl, TP Orthodontics). The cytotoxicity essay was performed using cell cultures (L-929 line cells, mouse fibroblast) that were submitted to the cell viability test with neutral red ("dye-uptake") at 1, 2, 3, 7 and 28 days. Analysis of variance (ANOVA) with 1-way analysis followed by the Tukey post hoc test were employed (p<.05). The results showed no statistically significant differences between Groups UK, TP and AO in all experimental times (p>.05). There was a statistically differences (p<.05) between the viability cell of Groups UK, TP and AO with the Groups O, M, TD and TC at 1, 2, 3 and 7 days. There was no statistically differences (p>.05) between the groups O, M and TD at in all experimental times. It can be concluded that the nonlatex elastomeric ligatures of Unitek, TP Orthodontics, and American Orthodontics showed higher cell viability compared to others ligatures.

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Development of a model to demonstrate photosensitizer‐mediated viral inactivation in blood

Cited by 96 publications

References 8 publications

Evaluation of the cytotoxicity of polyurethane and non-latex orthodontic chain elastics

Evaluation of the cytotoxicity of polyurethane and non-latex orthodontic chain elastics

Antiviral activity of extracts from Brazilian seaweeds against herpes simplex virus

Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

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