Development of a Monoclonal Antibody-Based Immunochemical Assay for Liquiritin and Its Application to the Quality Control of Licorice Products

Fujii, Shunsuke; Morinaga, Osamu; Uto, Takuhiro; Nomura, Shuichi; Shoyama, Yukihiro

doi:10.1021/jf404731z

Cited by 36 publications

(36 citation statements)

References 29 publications

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“…Flavonoids is another another group of bioactive constituents in G. uralensis , of which liquiritin, liquiritgenin, isoliquirtin, isoliquiritigenin, and licochalcone ( Figure 1 ) represent the major ones (Asl and Hosseinzadeh, 2008; Fujii et al, 2014). Effects of AMF on the flavonoids in liquorice under nutrient deficiency have not been thoroughly explored.…”

Section: Introductionmentioning

confidence: 99%

Glomus mosseae Inoculation Improves the Root System Architecture, Photosynthetic Efficiency and Flavonoids Accumulation of Liquorice under Nutrient Stress

et al. 2017

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The poor quality and low productivity of cultivated liquorice (Glycyrrhiza uralensis) continues to put pressure on wild plant populations. As arbuscular mycorrhizal fungi are known to support plant growth and in some cases even to enhance the accumulation of valuable molecules in the plant, the effect of Glomus mosseae on the growth and active ingredient contents was evaluated in liquorice plants grown under nutrient deficiency. We created a nutrient-deficient environment by mixing paddy soil, washed river sand, and pumice at a ratio of 1:5:1. Our results showed that the inoculation of pot-grown liquorice plants with G. mosseae significantly increased the shoot and root biomass (by 25- and 17-folds, respectively) and the contents of glycyrrhizic acid, liquiritin, isoliquiritin, and isoliquiritigenin in the main root (by 1.6-, 4.8-, 6.5-, and 4.4-folds, respectively). Both isoliquiritin and isoliquiritigenin were detectable in the lateral roots of the plants inoculated with G. mosseae, but not in plants without G. mosseae inoculation. G. mosseae inoculation improved the features of the root system and increased photosynthetic efficiency of liquorice. The uptake of P and K by liquorice increased when G. mosseae was inoculated, leading to the depletion of these macronutrients in the soil; G. mosseae also improved the availability of Mg, Cu, Zn, and Mn. Based on these results, we concluded that the inoculation of liquorice plants with G. mosseae is beneficial, particularly for those grown in nutrient-deficient soil, and such positive effect is related to the improvement of the root system and an increased photosynthetic efficiency.

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Section: Introductionmentioning

confidence: 99%

Glomus mosseae Inoculation Improves the Root System Architecture, Photosynthetic Efficiency and Flavonoids Accumulation of Liquorice under Nutrient Stress

et al. 2017

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“…Quality control of licorice root is needed to assure uniformity in the concentrations of these compounds, which can differ according to licorice species, growth place, and harvest season, and so on. To this end, we previously prepared MAbs against GC [4] and Liq [16] and developed an eastern blotting system for detection of GC and Liq and their aglycones (glycyrrhetinic acid and liquiritigenin, respectively) [17] in order to make evident the resource of licorice for further researches. In this method, crude licorice extract and/or traditional Chinese medicine extract are developed on a Thin acrosome reaction in sperm.…”

Section: Components Of Licorice and Quantitative And Qualitative Controlmentioning

confidence: 99%

In vitro Fertilization with Mouse Sperm Activated by Components of Licorice Root Extract

Tung¹,

Tanaka²,

Tsujimura³

et al. 2016

Nat Prod Chem Res

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Licorice (Glycyrrhiza species) is an important natural product that is frequently used in traditional Chinese medicine. In this review article, we discuss the quality control of licorice using monoclonal antibodies against the licorice components glyzyrrhizin (GC) and liquiritin (Liq); the monitoring of licorice extract activity using an assay system in mice; and the isolation of two active phenolic components, isolitiritigenin and formononetin, and their effectiveness for improving in vitro fertilization in mice. Finally, we present the outstanding questions and potential applications of licorice root extract.

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“…Furthermore, a new staining system, eastern blotting was developed [15] and immunoaffinity isolation of glycyrrhizin resulting in glycyrrhizin-knockout extract, which can be used to survey the real activity of glycyrrhizin in licorice [16,17]. Regarding flavonoid, anti-liquiritin MAb [18] and eastern blotting [19] was developed. Since the above knowledge of components in licorice have been accumulated and also Tanaka et al have been investigating about proteins [20][21][22] and mitochondria [23] related to fertilization using reproducing assay system, we have started to survey in vitro fertilization (IVF) active components in licorice.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Fertilization Activators for Future

Tanaka¹,

Wada²,

Tung³

et al. 2017

Biological Activities and Action Mechanisms of Licorice Ingredients

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Artificial insemination is an indispensable technology for cattle breeding and is used for treating infertility in humans. Thus, new or improved methods are needed to increase the efficiency of artificial insemination. In vitro fertilization (IVF) has been developed in mice. Although many mouse lines produced using IVF have been preserved by freezing embryos and/or fertilized eggs, the more efficient IVF using freezing preservation or long-term refrigeration of sperm is expected to preserve mouse lines more easily. In this chapter, we introduce the active compounds in licorice to improve the rate of IVF. We previously reported that the rate of IVF in mice was improved by adding a water extract of licorice (Glycyrrhiza uralensis), but not glycyrrhizin, to the artificial insemination culture medium. Recently, we analyzed the active ethyl acetate fraction containing high levels of flavonoids. This fraction was further purified by bioassay-guided separation to isolate isoliquiritigenin and formononetin, which contributed to the improved rate of IVF. Isoliquiritigenin and formononetin may be useful therapeutic agents for infertility treatment.

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Development of a Monoclonal Antibody-Based Immunochemical Assay for Liquiritin and Its Application to the Quality Control of Licorice Products

Cited by 36 publications

References 29 publications

Glomus mosseae Inoculation Improves the Root System Architecture, Photosynthetic Efficiency and Flavonoids Accumulation of Liquorice under Nutrient Stress

Glomus mosseae Inoculation Improves the Root System Architecture, Photosynthetic Efficiency and Flavonoids Accumulation of Liquorice under Nutrient Stress

In vitro Fertilization with Mouse Sperm Activated by Components of Licorice Root Extract

In Vitro Fertilization Activators for Future

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