Development of a mouse limb transplantation model

Zhang, Feng; De, Shi; Kryger, Zol B.; Moon, Wong; Lineaweaver, William C.; Buncke, Harry J.

doi:10.1002/(sici)1098-2752(1999)19:5<209::aid-micr1>3.0.co;2-5

Cited by 28 publications

(28 citation statements)

References 10 publications

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“…However, this accepted standard technique results in a higher rate of thrombosis (4) (15% to 20%), perhaps because of endothelial injury during the anastomosis and long anastomotic times (30-60 min) (4, 6). Since 1995, several modified techniques have been developed in anastomosis of small vessels, which include end to side suture anastomosis(6, 7), a temporary assisting suspension suture technique(8), the sleeve technique(10), and an “underwater” anastomosis by intermittent irrigation(15). However, the technical difficulty of this anastomosis still exists, which requires experienced microsurgical skills and long-term training.…”

Section: Discussionmentioning

confidence: 99%

A simplified cuff technique for abdominal aortic transplantation in mice

Zhu

Esckilsen

Atkinson

et al. 2016

Journal of Surgical Research

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Section: Discussionmentioning

confidence: 99%

A simplified cuff technique for abdominal aortic transplantation in mice

Zhu

Esckilsen

Atkinson

et al. 2016

Journal of Surgical Research

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“…9. Next, continue to divide the individual ventral (gracilis and medial thigh muscles) and dorsal muscle groups 20 proximally at the level of the mid-thigh to separate the native hind limb from the recipient animals using scissors. 10.…”

Section: Removal Of the Hind Limbmentioning

confidence: 99%

Orthotopic Hind Limb Transplantation in the Mouse

Furtmüller

Grahammer

et al. 2016

JoVE

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In vivo animal model systems, and in particular mouse models, have evolved into powerful and versatile scientific tools indispensable to basic and translational research in the field of transplantation medicine. A vast array of reagents is available exclusively in this setting, including mono- and polyclonal antibodies for both diagnostic and interventional applications. In addition, a vast number of genotyped, inbred, transgenic, and knock out strains allow detailed investigation of the individual contributions of humoral and cellular components to the complex interplay of an immune response and make the mouse the gold standard for immunological research. Vascularized Composite Allotransplantation (VCA) delineates a novel field of transplantation using allografts to replace "like with like" in patients suffering traumatic or congenital tissue loss. This surgical methodological protocol shows the use of a non-suture cuff technique for super-microvascular anastomosis in an orthotopic mouse hind limb transplantation model. The model specifically allows for comparison between established paradigms in solid organ transplantation with a novel form of transplants consisting of various different tissue components. Uniquely, this model allows for the transplantation of a viable vascularized bone marrow compartment and niche that have the potential to exert a beneficial effect on the balance of immune acceptance and rejection. This technique provides a tool to investigate alloantigen recognition and allograft rejection and acceptance, as well as enables the pursuit of functional nerve regeneration studies to further advance this novel field of transplantation.

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“…Right hind limbs of donor and recipient animals, including bone, muscle, femoral vessels, sciatic nerve, saphenous nerve, and skin, were amputated at the mid-femoral level as discussed in our previous rat model 15 and as Zhang et al described. 16 Donor hind limbs were orthotopically transplanted onto recipients. Osteosynthesis was achieved using a 25-gauge needle (about 8-to 10-mm length) as an intramedullary rod.…”

Section: Surgical Techniquementioning

confidence: 99%

“…Hypovolemic shock has been considered a major reason for animal death post limb transplant. 16 Thus, our initial procedures (groups 1 and 2) utilized 0.95 to 1.15 mL of the total supplemented fluid over the course of surgery ( Table 2). As this volume of fluid resulted in a high death rate (40%-43%), we considered hypervolemic syndrome as an additional complication resulting in death.…”

Section: Transplantation Survival Rates and Other Complicationsmentioning

confidence: 99%

Robust Axonal Regeneration in a Mouse Vascularized Composite Allotransplant Model Undergoing Delayed Tissue Rejection

Yan

Wood

Moore

et al. 2016

Hand (New York, N,Y.)

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Background: Nerve regeneration in vascularized composite allotransplantation (VCA) is not well understood. Allogeneic transplant models experience complete loss of nerve tissue and axonal regeneration without immunosuppressive therapy. The purpose of this study was to determine the impact of incomplete immunosuppression on nerve regeneration. Methods: In this study, transgenic mice (4 groups in total) with endogenous fluorescent protein expression in axons (Thy1-YFP) and Schwann cells (S100-GFP) were used to evaluate axonal regeneration and Schwann cell (SC) migration in orthotopic-limb VCA models with incomplete immunosuppression using Tacrolimus (FK506). Survival and complication rates were assessed to determine the extent of tissue rejection. Nerve regeneration was assessed using serial imaging of axonal progression and SC migration and viability. Histomorphometry quantified the extent of axonal regeneration. Results: Incomplete immunosuppression with FK506 resulted in delayed rejection of skin, muscle, tendon, and bone in the transplanted limb. In contrast, the nerve demonstrated robust axonal regeneration and SC viability based on strong fluorescent protein expression by SCs and axons in transgenic donors and recipients. Total myelinated axon numbers measured at 8 weeks were comparable in all VCA groups and not statistically different from the syngeneic donor control group. Conclusions: Our data suggest that nerve and SCs are much weaker antigens compared with skin, muscle, tendon, and bone in VCA. To our knowledge, this study is the first to prove the weak antigenicity of nerve tissue in the orthotopic VCA mouse model.

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Development of a mouse limb transplantation model

Cited by 28 publications

References 10 publications

A simplified cuff technique for abdominal aortic transplantation in mice

A simplified cuff technique for abdominal aortic transplantation in mice

Orthotopic Hind Limb Transplantation in the Mouse

Robust Axonal Regeneration in a Mouse Vascularized Composite Allotransplant Model Undergoing Delayed Tissue Rejection

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