Development of a multicomponent method for<i>Fusarium</i>toxins using LC-MS/MS and its application during a survey for the content of T-2 toxin and deoxynivalenol in various feed and food samples

Biselli, Scarlett; Hummert, Christian

doi:10.1080/02652030500158617

Cited by 117 publications

(77 citation statements)

References 19 publications

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“…13) They showed that DON contamination was high (maximum level = 2350 ng/g), but the concentration of T-2 was lower than that in our study (maximum level = 0.95 ng/g). Schollenberger et al 18) showed that NIV, DON, HT-2, T-2 and ZEN occurred in foodstuffs marketed in Germany with DON the most commonly observed.…”

Section: Analysis Of Trs and Zen In Biscuits Based On Wheatmentioning

confidence: 69%

“…5,6) Co-contamination of Fusarium mycotoxins (TRs and ZEN) occurs worldwide in agricultural commodities and consumption of these has caused several outbreaks of intoxication in human and animal populations. [7][8][9][10][11][12] With the development of highly sensitive and simultaneous analytical methods, many reports regarding the co-contamination of Fusarium mycotoxins in processed cereal foods have been reported in Europe and North America, [13][14][15] but relatively little work is available for Asian countries despite these depending heavily on imported wheat and wheat-derived products. Thus, an accurate determination of processed food contaminated with these toxins is an urgent need for C 2010 The Pharmaceutical Society of Japan food supply.…”

Section: Iintroductionmentioning

confidence: 99%

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A Survey of the Occurrence of Fusarium Mycotoxins in Biscuits in Japan by Using LC/MS

Tanaka¹,

Sugita-Konishi²,

Takino

et al. 2010

JOURNAL OF HEALTH SCIENCE

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Section: Analysis Of Trs and Zen In Biscuits Based On Wheatmentioning

confidence: 69%

Section: Iintroductionmentioning

confidence: 99%

A Survey of the Occurrence of Fusarium Mycotoxins in Biscuits in Japan by Using LC/MS

Tanaka¹,

Sugita-Konishi²,

Takino

et al. 2010

JOURNAL OF HEALTH SCIENCE

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“…Aflatoxins were separated in a UPLC instrument equipped with a matching UPLC Quaternary Solvent Manager, UPLC Sample Manager, UPLC Fluorescent Detector, and a C18 2.1 mm × 50 mm, 1.7 m column. Aflatoxin identity was determined by obtaining their UV and mass-spectral data and comparing them with published data [34]. Aflatoxin concentrations in samples were determined by reference to peak areas of commercial standards.…”

Section: Seed Inoculations and Aflatoxin Analysismentioning

confidence: 99%

Characterization of small RNA populations in non-transgenic and aflatoxin-reducing-transformed peanut

et al. 2017

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Power, Imana L.; Dang, Phat M.; Sobolev, Victor S.; Orner, Valerie; Powell, Joseph L.; Lamb, Marshall C.; and Arias, Renée S., "Characterization of small RNA populations in non-transgenic andaflatoxin-reducing-transformed peanut" (2017 t r a c tAflatoxin contamination is a major constraint in food production worldwide. In peanut (Arachis hypogaea L.), these toxic and carcinogenic aflatoxins are mainly produced by Aspergillus flavus Link and A. parasiticus Speare. The use of RNA interference (RNAi) is a promising method to reduce or prevent the accumulation of aflatoxin in peanut seed. In this study, we performed high-throughput sequencing of small RNA populations in a control line and in two transformed peanut lines that expressed an inverted repeat targeting five genes involved in the aflatoxin-biosynthesis pathway and that showed up to 100% less aflatoxin B 1 than the controls. The objective was to determine the putative involvement of the small RNA populations in aflatoxin reduction. In total, 41 known microRNA (miRNA) families and many novel miRNAs were identified. Among those, 89 known and 10 novel miRNAs were differentially expressed in the transformed lines. We furthermore found two small interfering RNAs derived from the inverted repeat, and 39 sRNAs that mapped without mismatches to the genome of A. flavus and were present only in the transformed lines. This information will increase our understanding of the effectiveness of RNAi and enable the possible improvement of the RNAi technology for the control of aflatoxins.

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“…C 8 , C 18 ), strong cation or anion exchangers (SCX, SAX) or polymeric materials with combined properties. Modern clean-up procedures employ multifunctional MycoSep Õ (Krska 1998;Radová et al 1998;Biselli and Hummert 2005;Ren et al 2007) or immunoaffinity columns (IAC) (Krska 1998), although these methods are more expensive than conventional clean-up methodologies. MycoSep Õ columns contain a mixture of charcoal, ion-exchange resins and other materials and are suitable for aflatoxins, trichothecenes, ochratoxins, zearalenone, moniliformin and patulin (Romerlabs 2007).…”

Section: Sample Preparation and Clean-upmentioning

confidence: 99%

Mycotoxin analysis: An update

Krska¹,

Schubert-Ullrich²,

Molinelli³

et al. 2008

Food Additives & Contaminants: Part A

312

215

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Mycotoxin contamination of cereals and related products used for feed can cause intoxication, especially in farm animals. Therefore, efficient analytical tools for the qualitative and quantitative analysis of toxic fungal metabolites in feed are required. Current methods usually include an extraction step, a clean-up step to reduce or eliminate unwanted co-extracted matrix components and a separation step with suitably specific detection ability. Quantitative methods of analysis for most mycotoxins use immunoaffinity clean-up with high-performance liquid chromatography (HPLC) separation in combination with UV and/or fluorescence detection. Screening of samples contaminated with mycotoxins is frequently performed by thin layer chromatography (TLC), which yields qualitative or semi-quantitative results. Nowadays, enzyme-linked immunosorbent assays (ELISA) are often used for rapid screening. A number of promising methods, such as fluorescence polarization immunoassays, dipsticks, and even newer methods such as biosensors and non-invasive techniques based on infrared spectroscopy, have shown great potential for mycotoxin analysis. Currently, there is a strong trend towards the use of multi-mycotoxin methods for the simultaneous analysis of several of the important Fusarium mycotoxins, which is best achieved by LC-MS/MS (liquid chromatography with tandem mass spectrometry). This review focuses on recent developments in the determination of mycotoxins with a special emphasis on LC-MS/MS and emerging rapid methods.

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Development of a multicomponent method forFusariumtoxins using LC-MS/MS and its application during a survey for the content of T-2 toxin and deoxynivalenol in various feed and food samples

Cited by 117 publications

References 19 publications

A Survey of the Occurrence of Fusarium Mycotoxins in Biscuits in Japan by Using LC/MS

A Survey of the Occurrence of Fusarium Mycotoxins in Biscuits in Japan by Using LC/MS

Characterization of small RNA populations in non-transgenic and aflatoxin-reducing-transformed peanut

Mycotoxin analysis: An update

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