Development of a multimodal foveated endomicroscope for the detection of oral cancer

Shadfan, Adam; Darwiche, Hawraa; Blanco, Jesús; Gillenwater, Ann M.; Richards‐Kortum, Rebecca; Tkaczyk, Tomasz S.

doi:10.1364/boe.8.001525

Cited by 16 publications

(10 citation statements)

References 37 publications

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“… 2 – 6 Other proof-of-concepts measured the oxygen saturation (OS) of various organs 7 ; assessed the presence of molecules such as cholesterol, melanin, and hemoglobin 8 , 9 ; enhanced the surgeon's vision in oncologic surgery and laparoscopy 10 – 13 ; predicted hemorrhagic shock and appraising hemodynamics 14 – 16 ; classified corneal injury 17 ; augmented contrast for histologic examinations 18 , 19 ; and detected neoplasms of the skin, mouth, colon, brain, and others. 3 , 5 , 20 – 22 In ophthalmology, HSI can be used to assess the state and distribution of chromophores, such as cytochrome C, and assess the metabolic status of hemoglobin in the context of retinal blood vessel oxygenation. Such information is valuable to diagnose and monitor various ocular diseases including age-related macular degeneration (ARMD), glaucoma, and diabetic retinopathy.…”

Section: Introductionmentioning

confidence: 99%

Hyperspectral Imaging and the Retina: Worth the Wave?

Lemmens

Eijgen

Keer

et al. 2020

Trans. Vis. Sci. Tech.

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Purpose: Hyperspectral imaging is gaining attention in the biomedical field because it generates additional spectral information to study physiological and clinical processes. Several technologies have been described; however an independent, systematic literature overview is lacking, especially in the field of ophthalmology. This investigation is the first to systematically overview scientific literature specifically regarding retinal hyperspectral imaging. Methods: A systematic literature review was conducted, in accordance with PRISMA Statement 2009 criteria, in four bibliographic databases: Medline, Embase, Cochrane Database of Systematic Reviews, and Web of Science. Results: Fifty-six articles were found that meet the review criteria. A range of techniques was reported: Fourier analysis, liquid crystal tunable filters, tunable laser sources, dualslit monochromators, dispersive prisms and gratings, computed tomography, fiber optics, and Fabry-Perrot cavity filter covered complementary metal oxide semiconductor. We present a narrative synthesis and summary tables of findings of the included articles, because methodologic heterogeneity and diverse research topics prevented a meta-analysis being conducted. Conclusions: Application in ophthalmology is still in its infancy. Most previous experiments have been performed in the field of retinal oximetry, providing valuable information in the diagnosis and monitoring of various ocular diseases. To date, none of these applications have graduated to clinical practice owing to the lack of sufficiently large validation studies. Translational Relevance: Given the promising results that smaller studies show for hyperspectral imaging (e.g., in Alzheimer's disease), advanced research in larger validation studies is warranted to determine its true clinical potential.

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Section: Introductionmentioning

confidence: 99%

Hyperspectral Imaging and the Retina: Worth the Wave?

Lemmens

Eijgen

Keer

et al. 2020

Trans. Vis. Sci. Tech.

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“…Advances in nanotechnology have enabled multiplexed molecular profiling of tumors for early detection and intraoperative guidance of tumor resection . A variety of nanoparticles (NPs) are now available for use as contrast agents in medical imaging; these NPs include quantum dots, carbon nanotubes, gold NPs, and nanowires .…”

Section: Introductionmentioning

confidence: 99%

Microscopic investigation of" topically applied nanoparticles for molecular imaging of fresh tissue surfaces

Kang

Wang

et al. 2018

Journal of Biophotonics

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Previous studies have shown that functionalized nanoparticles (NPs) topically applied on fresh tissues are able to rapidly target cell-surface protein biomarkers of cancer. Furthermore, studies have shown that a paired-agent approach, in which an untargeted NP is co-administered with a panel of targeted NPs, controls for the nonspecific behavior of the NPs, enabling quantitative imaging of biomarker expression. However, given the complexities in nonspecific accumulation, diffusion, and chemical binding of targeted NPs in tissues, studies are needed to better understand these processes at the microscopic scale. Here, fresh tissues were stained with a paired-agent approach, frozen, and sectioned to image the depth-dependent accumulation of targeted and untargeted NPs. The ratio of targeted-to-untargeted NP concentrations-a parameter used to distinguish between tumor and benign tissues-was found to diminish with increasing NP diffusion depths due to nonspecific accumulation and poor washout. It was then hypothesized and experimentally demonstrated that larger NPs would exhibit less diffusion below tissue surfaces, enabling higher targeted-to-untargeted NP ratios. In summary, these methods and investigations have enabled the design of NP agents with improved sensitivity and contrast for rapid molecular imaging of fresh tissues.

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“…This endoscope could acquire datacubes (350×350 pixels×48 channels, spatial resolution 100 µm) at a speed of 5.2 cubes/s for reflectance and fluorescence imaging 105 . Another recent study presented a multimodal endomicroscope that combined high-resolution confocal fluorescence imaging with wide-field snapshot multispectral imaging 120 . Ex vivo imaging of human oral tissue specimens demonstrated the capability of the endomicroscope to rapidly localize abnormal lesions within a 5-mm field of view followed by high-resolution multispectral imaging to determine the presence and degree of neoplasia 120 .…”

Section: Imaging Systemsmentioning

confidence: 99%

“…Another recent study presented a multimodal endomicroscope that combined high-resolution confocal fluorescence imaging with wide-field snapshot multispectral imaging 120 . Ex vivo imaging of human oral tissue specimens demonstrated the capability of the endomicroscope to rapidly localize abnormal lesions within a 5-mm field of view followed by high-resolution multispectral imaging to determine the presence and degree of neoplasia 120 . These techniques can potentially be used to enable surgical guidance, early cancer detection, and molecular profiling/staging of diseases.…”

Section: Imaging Systemsmentioning

confidence: 99%

Multiplexed Optical Imaging of Tumor-Directed Nanoparticles: A Review of Imaging Systems and Approaches

Wang¹,

Reder²,

Kang³

et al. 2017

Nanotheranostics

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In recent decades, various classes of nanoparticles have been developed for optical imaging of cancers. Many of these nanoparticles are designed to specifically target tumor sites, and specific cancer biomarkers, to facilitate the visualization of tumors. However, one challenge for accurate detection of tumors is that the molecular profiles of most cancers vary greatly between patients as well as spatially and temporally within a single tumor mass. To overcome this challenge, certain nanoparticles and imaging systems have been developed to enable multiplexed imaging of large panels of cancer biomarkers. Multiplexed molecular imaging can potentially enable sensitive tumor detection, precise delineation of tumors during interventional procedures, and the prediction/monitoring of therapy response. In this review, we summarize recent advances in systems that have been developed for the imaging of optical nanoparticles that can be heavily multiplexed, which include surface-enhanced Raman-scattering nanoparticles (SERS NPs) and quantum dots (QDs). In addition to surveying the optical properties of these various types of nanoparticles, and the most-popular multiplexed imaging approaches that have been employed, representative preclinical and clinical imaging studies are also highlighted.

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Development of a multimodal foveated endomicroscope for the detection of oral cancer

Cited by 16 publications

References 37 publications

Hyperspectral Imaging and the Retina: Worth the Wave?

Hyperspectral Imaging and the Retina: Worth the Wave?

Microscopic investigation of" topically applied nanoparticles for molecular imaging of fresh tissue surfaces

Multiplexed Optical Imaging of Tumor-Directed Nanoparticles: A Review of Imaging Systems and Approaches

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