Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites

Chmayssem, Ayman; Verplanck, Nicolas; Tanase, Constantin Edi; Costa, Guillaume; Monsalve-Grijalba, Karen; Amigues, Simon; Alias, Mélanie; Gougis, Maxime; Mourier, Véronique; Vignoud, Séverine; Ghaemmaghami, Amir M.; Mailley, Pascal

doi:10.1016/j.talanta.2021.122275

Cited by 24 publications

(26 citation statements)

References 68 publications

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“…This may explain the higher sensitivity to H 2 O 2 in William's medium [27]. So far, comparing to H 2 O 2 detection in PBS medium, the hydrogen peroxide sensors show a close detection limit in cell culture media under hydrodynamic conditions and are estimated to be a few μM as already reported in our previous work (in the range of 8–20 μM) [4].…”

Section: Resultssupporting

confidence: 71%

“…Lactate levels were recorded using amperometric enzymatic biosensors (electrodes 5 and 6) that embed the enzyme lactate oxidase (LOx) [4, 32]. LOx is immobilized at the surface of a PB electrode through crosslinking using Glutaraldehyde and BSA.…”

Section: Resultsmentioning

confidence: 99%

“…The 2D and 3D design of the multiparametric electrochemical (bio)sensing platform has already been described in our previous work [4]. Briefly, this printed circuit board (PCB) integrates one reference electrode (RE, 3 mm), one annular counter‐electrode (CE, Internal diameter of 8.46 mm, External Diameter of 9.85 mm) and eight working electrodes (WE, diameter of 1.6 mm) (Figure 1a).…”

Section: Methodsmentioning

confidence: 99%

“…For the multiparametric (bio)sensing platform, four parameters are monitored as follows: pH, H 2 O 2 , NO/NO 2 − and lactate. For these parameters, the working electrodes are functionalized by pairs by screen‐printing or drop casting of the different sensitive layers under clean room conditions [4]. In terms of the electrodes materials, our strategy consists of selecting processable materials in the view of industrializing the (bio)sensing platform.…”

Section: Methodsmentioning

confidence: 99%

“…More particularly, the PANBioRA system integrates a cytotoxicity-assessment microfluidic-module composed of a cell culture chamber connected to two analytical modules enabling respectively the monitoring of inflammatory biomarkers and of metabolites related to the cell stress [1][2][3]. The latter (including NO, lactate, pH and H 2 O 2 ) are monitored in line using a multiparametric electroanalytical platform [4]. However, according to the cultured cells, specific media that differs widely by their composition have to be used [5] and might significantly impact the electrochemical (bio)sensors response.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the Impact of Classical Cell‐culture Media on the Response of Electrochemical Sensors

et al. 2022

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Biocompatibility testing is usually performed through staining and imaging of cell lines. We propose here to monitor cytotoxicity through real‐time measurement of metabolites specifically issued from cell stress behaviour using a multiparametric electrochemical (bio)sensing platform. However, the composition of culture media varies widely according to the requirements of the utilized cell lines. This matter may have significant effects on the sensor's sensitivity. With this mind, the sensitivity of four electrochemical (bio)sensors (pH, hydrogen peroxide, nitric oxide/nitrite (NO and its by‐product) and lactate) is investigated in different cell culture media. The main culture media studied were Minimum Essential Medium Eagle (MEM), Dulbecco's Modified Eagle Medium (DMEM), Williams’ Medium E and RPMI 1640 medium that were the recommended culture media for the cell types to be monitored. This work shows the impact of the different cell culture media on the performances of the different sensors (limit of detection, sensitivity, selectivity, response time and dynamic range). More particularly, FBS strongly impacts the response of the amperometric (bio)sensors. Then, cellular viability testing was effected within optimized medium (FBS content) for electrochemical sensor read‐outs in the case of short‐term cultures (one day) devoted to cytotoxicity testing. Real‐time electrochemical monitoring provides important additional information about cell behaviour during biocompatibility testing that might be further implemented in different settings including pharmaceutical efficacy and biomaterials applications.

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Section: Resultssupporting

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of the Impact of Classical Cell‐culture Media on the Response of Electrochemical Sensors

et al. 2022

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Organ‐On‐A‐Chip Devices: Technology Progress and Challenges

Obeid,

Yammine,

El‐Nakat

et al. 2024

ChemBioChem

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Organ‐On‐a‐Chip (OOC) is a multichannel 3D‐microfluidic cell‐culture system incorporated in a chip that simulates the behavior of an organ. This technology relies on a multidisciplinary science that benefits from and contributes in the progress of many fields including microbiology, microfluidics, biomaterials, and bioengineering. This review article summarizes the progress and achievements of various organ‐on‐chip technologies. It highlights the significant advantages of this technology in terms of reducing animal testing and providing personalized medical responses. In addition, this paper demonstrates how OOC is becoming a promising and powerful tool in pharmaceutical research to combat diseases. It predicts not only the effects of drugs on the target organs but also, using body‐on‐a‐chip systems, it may provide insights into the side effects of the drug delivery on the other organs. Likewise, the models used for the construction of various organ‐on‐a‐chip devices are investigated along with the design and materials of microfluidic devices. For each OOC, the integrated monitoring devices within the chips (e. g., sensors and biosensors) are discussed. We also discuss the evolution of FDA regulations and the potential in the near future for integrating OOCs into protocols that support and reduce the need for and the failure rates in preclinical and clinical studies.

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