Development of a multiplex real-time RT-PCR assay for detection of influenza A, influenza B, RSV and typing of the 2009-H1N1 influenza virus

Hymas, Weston; Mills, Alan; Ferguson, Sheri; Langer, Janine; She, Rosemary C.; Mahoney, Walt; Hillyard, David R.

doi:10.1016/j.jviromet.2010.03.020

Cited by 20 publications

(15 citation statements)

References 18 publications

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“…On a positive note, the lack of influenza illnesses in our study population likely reduced confounding regarding the potential effects of intercurrent influenza infections on antibody levels. Finally, similar to other studies, the precise etiology of ILI was often unknown, despite an extensive evaluation for a number of bacterial and viral pathogens using state-of-the-art PCR technology [48, 49]. …”

Section: Discussionmentioning

confidence: 70%

Durability of antibody responses after receipt of the monovalent 2009 pandemic influenza A (H1N1) vaccine among HIV-infected and HIV-uninfected adults

Crum-Cianflone

Iverson

Defang

et al. 2011

Vaccine

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Section: Discussionmentioning

confidence: 70%

Durability of antibody responses after receipt of the monovalent 2009 pandemic influenza A (H1N1) vaccine among HIV-infected and HIV-uninfected adults

Crum-Cianflone

Iverson

Defang

et al. 2011

Vaccine

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show abstract

“…NP aspirates were found to contain the highest amount of virus, followed by NS and TS. Hymas et al described a multiplex RT-PCR assay that detected influenza A, influenza B, RSV and also the pandemic influenza A H1N1 subtype 79 . The authors evaluated 143 specimens and showed that this assay identified pandemic H1N1 as well as the CDC assay.…”

Section: Swine Origin Influenza A/h1n1 2009mentioning

confidence: 99%

Molecular diagnosis of respiratory virus infections

Mahony¹,

Petrich²,

Śmieja³

2011

Critical Reviews in Clinical Laboratory Sciences

193

181

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The appearance of eight new respiratory viruses, including the SARS coronavirus in 2003 and swine-origin influenza A/H1N1 in 2009, in the human population in the past nine years has tested the ability of virology laboratories to develop diagnostic tests to identify these viruses. Nucleic acid based amplification tests (NATs) for respiratory viruses were first introduced two decades ago and today are utilized for the detection of both conventional and emerging viruses. These tests are more sensitive than other diagnostic approaches, including virus isolation in cell culture, shell vial culture (SVC), antigen detection by direct fluorescent antibody (DFA) staining, and rapid enzyme immunoassay (EIA), and now form the backbone of clinical virology laboratory testing around the world. NATs not only provide fast, accurate and sensitive detection of respiratory viruses in clinical specimens but also have increased our understanding of the epidemiology of both new emerging viruses such as the pandemic H1N1 influenza virus of 2009, and conventional viruses such as the common cold viruses, including rhinovirus and coronavirus. Multiplex polymerase chain reaction (PCR) assays introduced in the last five years detect up to 19 different viruses in a single test. Several multiplex PCR tests are now commercially available and tests are working their way into clinical laboratories. The final chapter in the evolution of respiratory virus diagnostics has been the addition of allelic discrimination and detection of single nucleotide polymorphisms associated with antiviral resistance. These assays are now being multiplexed with primary detection and subtyping assays, especially in the case of influenza virus. These resistance assays, together with viral load assays, will enable clinical laboratories to provide physicians with new and important information for optimal treatment of respiratory virus infections.

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“…NPA was found to contain the highest amount of virus followed by nasal swab and throat swab. Multiplex RT-PCR assays have also been developed to detect influenza A, influenza B and RSV, and identify the pandemic influenza A H1N1 subtype [47]. The authors evaluated 143 specimens and showed that this assay identified pandemic H1N1 as well as the CDC assay.…”

Section: Swine-origin Influenza H1n1 2009mentioning

confidence: 99%

Nucleic acid amplification-based diagnosis of respiratory virus infections

Mahony¹

2010

Expert Review of Anti-infective Therapy

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The appearance of eight new respiratory viruses in the human population in the past 9 years, including two new pandemics (SARS coronavirus in 2003 and swine-origin influenza A/H1N1 in 2009), has tested the ability of virology laboratories to develop diagnostic tests to identify these viruses. Nucleic acid amplification tests (NATs) that first appeared two decades ago have been developed for both conventional and emerging viruses and now form the backbone of the clinical laboratory. NATs provide fast, accurate and sensitive detection of respiratory viruses and have significantly increased our understanding of the epidemiology of these viruses. Multiplex PCR assays have been introduced recently and several commercial tests are now available. The final chapter in the evolution of respiratory virus diagnostics will be the addition of allelic discrimination and detection of single nucleotide polymorphisms associated with antiviral resistance to multiplex assays. These resistance assays together with new viral load tests will enable clinical laboratories to provide physicians with important information for optimal treatment of patients.

show abstract

Development of a multiplex real-time RT-PCR assay for detection of influenza A, influenza B, RSV and typing of the 2009-H1N1 influenza virus

Cited by 20 publications

References 18 publications

Durability of antibody responses after receipt of the monovalent 2009 pandemic influenza A (H1N1) vaccine among HIV-infected and HIV-uninfected adults

Durability of antibody responses after receipt of the monovalent 2009 pandemic influenza A (H1N1) vaccine among HIV-infected and HIV-uninfected adults

Molecular diagnosis of respiratory virus infections

Nucleic acid amplification-based diagnosis of respiratory virus infections

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