Development of a multispecies periodontal biofilm model within a stirred bioreactor

Prado, Maick Meneguzzo; Kovalski, Davi J; Torrez, Willy Bustillos; Bueno‐Silva, Bruno; Feres, Magda; Almeida, Josiane de; Porto, Luismar Marques

doi:10.1080/08927014.2020.1805600

Cited by 3 publications

(1 citation statement)

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“…Additionally, the biofilm produced by periodontal pathogens and associated bacteria including S. mutants and P. gingivalis decreases the effectiveness of antibiotic therapy [ 44 , 60 ]. Streptococcus species were the precursors of dental biofilms [ 61 ]. Patients with chronic periodontitis exhibited higher levels of S. mutans colonization in both saliva and subgingival plaque samples.…”

Section: Discussionmentioning

confidence: 99%

Postbiotic Metabolite of Lactiplantibacillus plantarum PD18 against Periodontal Pathogens and Their Virulence Markers in Biofilm Formation

et al. 2023

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Alternative methods to reduce infectious diseases caused by bacterial pathogens and their virulence factors, biofilm formations, have arisen to reduce the pressure on existing or currently developed disinfectants and antimicrobial agents. The current strategies for reducing the severity of periodontal pathogen-caused disease by using beneficial bacteria and their metabolites are highly desirable. Probiotic strains of lactobacilli related to foods from Thai-fermented foods were selected and their postbiotic metabolites (PM) were isolated with inhibitory activity on periodontal pathogens and their biofilm formation. The PM from Lactiplantibacillus plantarum PD18 (PD18 PM) with the highest antagonistic effect against Streptococcus mutans, Porphyromonas gingivalis, Tannerella forsythia and Prevotella loescheii was selected from 139 Lactobacillus isolates. The minimal inhibitory concentration (MIC) and minimum biofilm inhibitory concentration (MBIC) values of PD18 PM against the pathogens ranged from 1:2 to 1:4. The PD18 PM demonstrated the ability to prevent the biofilm formation of S. mutans and P. gingivalis by showing a significant reduction in viable cells, high percentages of biofilm inhibition at 92.95 and 89.68%, and the highest effective contact times at 5 and 0.5 min, respectively. L. plantarum PD18 PM showed potential as a promising natural adjunctive agent to inhibit periodontal pathogens and their biofilms.

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Section: Discussionmentioning

confidence: 99%

Postbiotic Metabolite of Lactiplantibacillus plantarum PD18 against Periodontal Pathogens and Their Virulence Markers in Biofilm Formation

et al. 2023

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Recent Updates on Microbial Biofilms in Periodontitis: An Analysis of In Vitro Biofilm Models

Prado

Figueiredo

Pimenta

et al. 2022

Advances in Experimental Medicine and Biology

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Characterization and Growth Kinetics of a Multispecies Periodontal Biofilm Developed in a Stirred Bioreactor

et al. 2020

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This study aimed to validate an in vitro periodontal biofilm model with complex subgingival microbial communities grown in a stirred bioreactor. Experimental biofilms were characterized and examined for their growth kinetics over time. After incubation under anaerobic conditions for 3 days, biofilm cultures formed on the substrate were collected and analyzed. The specific growth rate in the exponential phase was estimated to be 0.3915 h−1, with no significant differences in growth kinetics and CFU counts between the replicates (P > 0.05). The biofilm architecture was consistent in each time-point, with bacterial cells presenting different morphology and completely colonizing the cementum surface. To conclude, the three-day subgingival biofilm model developed in our study successfully mimicked the growth of complex microbial communities.

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Development of a multispecies periodontal biofilm model within a stirred bioreactor

Cited by 3 publications

References 58 publications

Postbiotic Metabolite of Lactiplantibacillus plantarum PD18 against Periodontal Pathogens and Their Virulence Markers in Biofilm Formation

Postbiotic Metabolite of Lactiplantibacillus plantarum PD18 against Periodontal Pathogens and Their Virulence Markers in Biofilm Formation

Recent Updates on Microbial Biofilms in Periodontitis: An Analysis of In Vitro Biofilm Models

Characterization and Growth Kinetics of a Multispecies Periodontal Biofilm Developed in a Stirred Bioreactor

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