Development of a neuronal pressure sensor

Heal, Richard; Rogers, Adrian; Lunt, George G.; Pointer, Steve A; Parsons, A.

doi:10.1016/s0956-5663(01)00236-6

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…1 ("Cellular Barosensitivity") and illustrated in Fig. 2 and elsewhere (90,138,150,153,192), neuronal barosensitivity, as defined by changes in firing rate, V m , and R in , occurs at 2.5-4 ATA and possibly as low as 100 mmHg (ϳ1.13 ATA or ϳ0.13 ATG) (2). Because relatively few studies have attempted to differentiate the effects of hydrostatic pressure from gas partial pressure in the mCNS, especially at PB Ͻ5 ATA (89), we would argue that it is premature to conclude that small changes in physical pressure do not alter neuronal function in the mCNS.…”

Section: Thermodynamic Reactions Vs Mechanical Forcesmentioning

confidence: 99%

“…Excitable cells in invertebrates are barosensitive to ambient pressures ranging from 4 to 10 ATA by using either true hydrostatic compression or He compression (34,47,48,192). Similarly, neurons in the rat brain stem are barosensitive to even smaller pressures of only 2.4-4 ATA He (58,150,153), and brief hydraulic pressures ranging from 1.17 to 4.0 ATA can alter neuronal excitability in dorsal root ganglion neurons (90,138,181). For example, Fig.…”

Section: Hydrostatic Compression Of the Brainmentioning

confidence: 99%

“…What is interesting, however, is that not all neurons in the mCNS are equally sensitive to moderate levels of hyperbaria (82,153). For example, initial reports indicate that hippocampal neurons do not respond to 3 ATA He or hydraulic pressure (82,90). Similarly, not all neurons in the solitary complex are sensitive to 2-4 ATA He (153).…”

Section: Hydrostatic Compression Of the Brainmentioning

confidence: 99%

“…The preceding discussion indicates that neuronal barosensitivity occurs over a very large range of pressure (89,90,114,130,138,150,153,192). It has been suggested that, depending on the level of hyperbaric pressure, different cellular mechanisms may be responsible for mediating cellular barosensitivity (41,94,129,131,136).…”

Section: Thermodynamic Reactions Vs Mechanical Forcesmentioning

confidence: 99%

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Neuronal sensitivity to hyperoxia, hypercapnia, and inert gases at hyperbaric pressures

Dean¹,

Mulkey²,

Garcia³

et al. 2003

Journal of Applied Physiology

175

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III. Neuronal sensitivity to hyperoxia, hypercapnia, and inert gases at hyperbaric pressures. J Appl Physiol 95: 883-909, 2003; 10.1152/japplphysiol.00920.2002.-As ambient pressure increases, hydrostatic compression of the central nervous system, combined with increasing levels of inspired PO 2, PCO2, and N2 partial pressure, has deleterious effects on neuronal function, resulting in O 2 toxicity, CO2 toxicity, N2 narcosis, and high-pressure nervous syndrome. The cellular mechanisms responsible for each disorder have been difficult to study by using classic in vitro electrophysiological methods, due to the physical barrier imposed by the sealed pressure chamber and mechanical disturbances during tissue compression. Improved chamber designs and methods have made such experiments feasible in mammalian neurons, especially at ambient pressures Ͻ5 atmospheres absolute (ATA). Here we summarize these methods, the physiologically relevant test pressures, potential research applications, and results of previous research, focusing on the significance of electrophysiological studies at Ͻ5 ATA. Intracellular recordings and tissue PO 2 measurements in slices of rat brain demonstrate how to differentiate the neuronal effects of increased gas pressures from pressure per se. Examples also highlight the use of hyperoxia (Յ3 ATA O 2) as a model for studying the cellular mechanisms of oxidative stress in the mammalian central nervous system. anesthesia; carbon dioxide toxicity; free radicals; high-pressure nervous syndrome; membrane potential; nitrogen narcosis; oxidative stress; oxygen toxicity; polarographic oxygen electrode MOST HUMANS ARE PHYSIOLOGICALLY adapted to live and work near sea level, where ambient pressure is ϳ1 atmosphere absolute (ATA).1 Nonetheless, we exploit a continuum of barometric pressure (PB) ranging from the near vacuum of outer space, which we survive during Space Shuttle extravehicular activity by wearing a 4.3 lb./in.2 absolute (psia) pressure suit (30,300 ft. pressure equivalent, 0.29 ATA) (120, 220), down to the summit of Mt. Everest at 29,029 ft., where PB is 0.31 ATA (221), to ocean depths as great as 2,300 ft. of sea water (fsw), where PB increases to ϳ70 ATA (18). These situations are the pressure extremes of our inhabitable environment, which only relatively few highly trained individuals have ever occupied.Military personnel, medical personnel, and other humans, however, frequently encounter levels of hyperbaric pressure (i.e., Ͼ1 ATA) of lesser degrees in their normal work environments. Examples of moderate hyperbaric environments (e.g., Ͻ5 ATA) include the following: patients and medical attendants undergoing hyperbaric O 2 therapy (HBOT) (38,203); diving with an underwater breathing apparatus for recreational, professional (oil and salvage companies), and combat purposes (89); simulated dry and wet dives for hyperbaric research and dive training (217); and working in the compressed atmosphere of a subterranean environment (117). Abnormal work environments resulting from catastrophic accident...

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Section: Thermodynamic Reactions Vs Mechanical Forcesmentioning

confidence: 99%

Section: Hydrostatic Compression Of the Brainmentioning

confidence: 99%

Section: Hydrostatic Compression Of the Brainmentioning

confidence: 99%

Section: Thermodynamic Reactions Vs Mechanical Forcesmentioning

confidence: 99%

See 2 more Smart Citations

Neuronal sensitivity to hyperoxia, hypercapnia, and inert gases at hyperbaric pressures

Dean¹,

Mulkey²,

Garcia³

et al. 2003

Journal of Applied Physiology

175

View full text Add to dashboard Cite

show abstract

“…We are attempting to recreate this circuit on a microcantilever-based bio-MEMS (bio-microelectromechanical systems) device. In vitro neuronal network models have multiple potential applications in advanced medical diagnostic, cell-based biosensors, neurological implants, and high throughput drug/toxin screening, as well as fundamental neurobiological studies (Gross et al 1995;Ravenscroft et al 1998;Scholl et al 2000;Heal et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Electrophysiological and immunocytochemical characterization of DRG neurons on an organosilane surface in serum-free medium

Liu

Rumsey

Das

et al. 2008

In Vitro Cell.Dev.Biol.-Animal

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We are attempting to recreate a stretch reflex circuit on a patterned Bio-MEMS (bio-microelectromechanical systems) chip with deflecting micro-cantilevers. The first steps to recreate this system is to be able to grow individual components of the circuit (sensory neuron, motoneuron, skeletal muscle, and muscle spindle) on a patternable, synthetic substrate coating the MEMS device. Sensory neurons represent the afferent portion of the stretch reflex arc and also play a significant role in transmitting the signal from the muscle spindle to the spinal cord motoneurons. We have utilized a synthetic silane substrate N-1[3-(trimethoxysilyl) propyl) diethylenetriamine (DETA) on which to grow and pattern the cells. DETA forms a self-assembled monolayer on a variety of silicon substrates, including glass, and can be patterned using photolithography. In this paper, we have evaluated the growth of sensory neurons on this synthetic silane substrate. We have investigated the immunocytochemical and electrophysiological properties of the sensory neurons on DETA and compared the resultant properties with a biological control substrate (ornithine/laminin). Immunocytochemical studies revealed the survival and growth of all three subtypes of sensory neurons: trkA, trkB, and trkC on both surfaces. Furthermore, whole-cell patch clamp recordings were used to study the electrophysiological properties of the sensory neurons on the two surfaces. There were no significant differences in the electrical properties of the neurons grown on either surface. This is the first study analyzing the immunocytochemical and electrophysiological properties of sensory neurons grown long-term in a completely defined environment and on a nonbiological substrate.

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Association of different prediction methods for determination of the efficiency and selectivity on neuron-based sensors

Prasad

Tuncel

Ozkan

2006

Biosensors and Bioelectronics

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Development of a neuronal pressure sensor

Cited by 6 publications

References 16 publications

Neuronal sensitivity to hyperoxia, hypercapnia, and inert gases at hyperbaric pressures

Neuronal sensitivity to hyperoxia, hypercapnia, and inert gases at hyperbaric pressures

Electrophysiological and immunocytochemical characterization of DRG neurons on an organosilane surface in serum-free medium

Association of different prediction methods for determination of the efficiency and selectivity on neuron-based sensors

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