Development of a new autofluorescence probe for the analysis of normal and tumour brain tissues

Siebert, R.; Thi, Meilinh; Jean, F; Charon, Y.; Collado-Hilly, Mauricette; Duval, M.; Mandat, Tomasz; Ménard, Laurent; Palfi, Stéphane; Tordjmann, Thierry

doi:10.1117/12.781311

Cited by 6 publications

(2 citation statements)

References 22 publications

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“…Because of this reduced contrast, the system's effective sensitivity (i.e., the ability the visualize PpIX) also decreases, even though the actual sensitivity (ability to “collect” PpIX fluorescence photons) remains high. The variability in autofluorescence is high, both between various tumors as well as within a single tumor . Therefore, WFM is occasionally able to detect PpIX signal at one tissue location but not at an adjacent spot (though PpIX is present in both regions).…”

Section: Resultsmentioning

confidence: 99%

Comparing high‐resolution microscopy techniques for potential intraoperative use in guiding low‐grade glioma resections

Meza

Wang

et al. 2015

Lasers Surg Med

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Background and Objectives Fluorescence image-guided surgery (FIGS), with contrast provided by 5-ALA-induced-PpIX, has been shown to enable a higher extent of resection of high-grade gliomas. However, conventional FIGS with low-power microscopy lacks the sensitivity to aid in low-grade glioma (LGG) resection because PpIX signal is weak and sparse in such tissues. Intraoperative high-resolution microscopy of PpIX fluorescence has been proposed as a method to guide LGG resection, where sub-cellular resolution allows for the visualization of sparse and punctate mitochondrial PpIX production in tumor cells. Here, we assess the performance of three potentially portable high-resolution microscopy techniques that may be used for the intraoperative imaging of human LGG tissue samples with PpIX contrast: high-resolution fiber-optic microscopy (HRFM), high-resolution wide-field microscopy (WFM), and dual-axis confocal (DAC) microscopy. Materials and Methods Thick unsectioned human LGG tissue samples (n = 7) with ALA-induced-PpIX contrast were imaged using three imaging techniques (HRFM, WFM, DAC). The average signal-to-background ratio (SBR) was then calculated for each imaging modality (5 images per tissue, per modality). Results HRFM provides the ease of use and portability of a flexible fiber bundle, and is simple and inexpensive to build. However, in most cases (6/7), HRFM is not capable of detecting PpIX signal from LGGs due to high autofluorescence, generated by the fiber bundle under laser illumination at 405 nm, which overwhelms the PpIX signal and impedes its visualization. WFM is a camera-based method possessing high lateral resolution but poor axial resolution, resulting in sub-optimal image contrast. Conclusions Consistent successful detection of PpIX signal throughout our human LGG tissue samples (n = 7), with an acceptable image contrast (SBR > 2), was only achieved using DAC microscopy, which offers superior image resolution and contrast that is comparable to histology, but requires a laser-scanning mechanism to achieve optical sectioning.

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Section: Resultsmentioning

confidence: 99%

Comparing high‐resolution microscopy techniques for potential intraoperative use in guiding low‐grade glioma resections

Meza

Wang

et al. 2015

Lasers Surg Med

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“…Next optimizations will include the improvement of the detection efficiency non-uniformity and the development of new detection heads. The ultimate version of the probe will associate simultaneous localization of radiolabelled tissues with measurement of laser-induced fluorescence so as to discriminate neoplastic from normal tissues more specifically (Siebert et al 2008). In combination with other monitoring devices used in the operating room, we hope that this new tool will be a valuable aid to assist surgeon's excise brain tumour tissue more completely.…”

Section: Discussionmentioning

confidence: 99%

Development of a positron probe for localization and excision of brain tumours during surgery

et al. 2009

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The survival outcome of patients suffering from gliomas is directly linked to the complete surgical resection of the tumour. To help the surgeons to delineate precisely the boundaries of the tumour, we developed an intraoperative positron probe with background noise rejection capability. The probe was designed to be directly coupled to the excision tool such that detection and removal of the radiolabelled tumours could be simultaneous. The device consists of two exchangeable detection heads composed of clear and plastic scintillating fibres. Each head is coupled to an optic fibre bundle that exports the scintillating light to a photodetection and processing electronic module placed outside the operative wound. The background rejection method is based on a real-time subtraction technique. The measured probe sensitivity for (18)F was 1.1 cps kBq(-1) ml(-1) for the small head and 3.4 cps kBq(-1) ml(-1) for the large head. The mean spatial resolution was 1.6 mm FWHM on the detector surface. The gamma-ray rejection efficiency measured by realistic brain phantom modelling of the surgical cavity was 99.4%. This phantom also demonstrated the ability of the probe to detect tumour discs as small as 5 mm in diameter (20 mg) for tumour-to-background ratios higher than 3:1 and with an acquisition time around 4 s at each scanning step. These results indicate that our detector could be a useful complement to existing techniques for the accurate excision of brain tumour tissue and more generally to improve the efficiency of radio-guided cancer surgery.

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Radioguided Surgery of Brain Tumors

Ménard

2011

Tumors of the Central Nervous System, Volume 3

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Development of a new autofluorescence probe for the analysis of normal and tumour brain tissues

Cited by 6 publications

References 22 publications

Comparing high‐resolution microscopy techniques for potential intraoperative use in guiding low‐grade glioma resections

Comparing high‐resolution microscopy techniques for potential intraoperative use in guiding low‐grade glioma resections

Development of a positron probe for localization and excision of brain tumours during surgery

Radioguided Surgery of Brain Tumors

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