Development of a new broad-specific monoclonal antibody with uniform affinity for aflatoxins and magnetic beads-based enzymatic immunoassay

Synthesis of hapten, production of monoclonal antibody, and development of immunoassay for ribavirin detection in chicken

“…KLH was used as the carrier protein because of higher immunogenicity in mammalian hosts (B. Li, Yuan, et al., 2018; X. Zhang et al., 2017). To verify the effectiveness of Hapten 4, Hapten 1 and Hapten 2 were also used as immunizing haptens.…”

Recent Advances in Conventional Methods and Electrochemical Aptasensors for Mycotoxin Detection

“…Broad-specific mAbs were developed for the detection of total aflatoxins (AFs) (AFB 1 , aflatoxin B 2 (AFB 2 ), aflatoxin G 1 (AFG 1 ) and aflatoxin G 2 (AFG 2 )), with a low half maximal inhibitory concentration (IC 50 ) in the range of 0.04 µg kg −1 to 0.06 µg kg −1 . This was achieved by incorporating an AFB 1 modified with O-carboxymethyl oxime (AFB 1 -CMO), which is known to produce broad-specific mAbs, as the hapten in antibody production, followed by cell fusion with hybridoma technology [ 28 ]. There are other commercial monoclonal antibody isolation techniques that are available, and they vary depending on the intended application of the antibodies.…”

Immunomagnetic bead-based biotin-streptavidin system for highly efficient detection of aflatoxin B1 in agricultural products

“…In addition, the BSA-IMB system, as an immobile phase pattern, has larger surface area than the at base of a microtiter plate, which can immobilize a high number of antibodies. 18,24 Moreover, the volume of the sample loaded in magnetic microspheres is larger than that in conventional ELISA, which enables more free targets to occupy the antibody binding sites and improves the sensitivity of the immunoassay. In summary, due to numerous potential advantages of BSA-IMB, it is a straightforward, rapid and highly efficient testing method.…”