Development of a new experimental model of saccular aneurysm by intra-arterial incubation of papain in rabbits

Abstract

Papain was capable of forming aneurysms with histological characteristics similar to those of elastase-induced aneurysms; however, a comparative study is necessary to determine whether the papain is superior to elastase in the production of experimental saccular aneurysms.

Main Models of Experimental Saccular Aneurysm in Animals

“…These alterations are similar to those found in elastase-induced aneurysms, in which the only difference is the degree of thrombosis, which was more remarkable in the papain-induced aneurysms [73].…”

“…To overcome this limitation, Chinese researchers tested an association between elastase and collagenase in the in vitro pre-digestion of an arterial pouch grafted onto the aortic arch of rabbits; however, that model exhibited a higher tendency to spontaneously rupture [72]. To produce saccular aneurysms larger than 5 mm, De Oliveira et al (2011) infused the papain enzyme successfully inside the right common carotid artery of rabbits [73].…”

“…Other innovations were the removal of the aortic arch and the supra-aortic trunks, direct measurement of the macroscopic dimensions of aneurysms and vessels using a caliper, and quantitative histological studies by means of histomorphometry in addition to a qualitative histological analysis [60,73].…”

Modelo experimental estável de aneurisma sacular em artéria carótida de suínos utilizando veia jugular interna

“…These models attempt to reproduce the histological, geometrical, hemodynamic and pathophysiological characteristics of cerebral aneurysms in humans 17 . These models should be suitable for hemodynamic and pathological studies and to evaluate the efficacy of therapeutic procedures 18,19 .…”