Development of a new extraction technique and HPLC method for the analysis of non-psychoactive cannabinoids in fibre-type Cannabis sativa L. (hemp)

Brighenti, Virginia; Pellati, Federica; Steinbach, Marleen; Maran, Davide; Benvenuti, Stefania

doi:10.1016/j.jpba.2017.05.049

Cited by 216 publications

(241 citation statements)

References 25 publications

Supporting

Mentioning

214

Contrasting

Unclassified

Order By: Relevance

“…The best combination in terms of relative area (area analyte/IS) was obtained using methanol as extraction solvent at room temperature and 2 extraction cycles of 5 min each, with a resulting total extraction time of 15 min (Figure 1). These results are in line with a recent study that investigated the use of different extraction methods (dynamic maceration, ultrasound, microwave and supercritical fluid extraction) for the analysis of cannabinoids from fibre-type cannabis varieties [38]. Recoveries calculated by comparing the concentrations of the extracted compounds with those from the MMC calibration curves at two different fortification levels showed an average recovery of 93 and 5.7% as coefficient of variation.…”

Section: Romano Andsupporting

confidence: 90%

Quality Traits of Medical Cannabis sativa L. Inflorescences and Derived Products Based on Comprehensive Mass-Spectrometry Analytical Investigation

Calvi¹,

Pavlović²,

Panseri³

et al. 2019

Recent Advances in Cannabinoid Research

View full text Add to dashboard Cite

Cannabis sativa L. has been cultivated throughout the world for industrial and medical purposes and is the most controversial plant ever exploited, with considerable discrepancies in the praise and disapproval it receives. Medical Cannabis prescriptions are on the increase in several countries where its therapeutic use is authorised due to its positive role in treating several pathologies even if it represents a multifaceted reality in terms of application. There are at least 550 identified compounds in C. sativa L., including more than 100 phytocannabinoids and 120 terpenes. The chemical complexity of its bioactive constituents highlights the need for standardised and well-defined analytical approaches able to characterise plant chemotype and herbal drug quality as well as to monitor the quality of pharmaceutical cannabis extracts and preparations. This research highlights the potential of using different analytical procedures involving the combination of headspacesolid-phase microextraction (HS-SPME) coupled to GC-MS and accelerated solvent extraction (ASE) coupled to high resolution mass-spectrometry (HPLC-Q Orbitrap®) for the indepth profiling of quality traits in authorised medical varieties of Cannabis sativa L. flos (Bediol®) and corresponding macerated oil preparations. This approach could add new knowledge to the field of "omic" analytical applications which are fundamental nowadays for Cannabis used for therapeutic remedies.

show abstract

Section: Romano Andsupporting

confidence: 90%

Quality Traits of Medical Cannabis sativa L. Inflorescences and Derived Products Based on Comprehensive Mass-Spectrometry Analytical Investigation

Calvi¹,

Pavlović²,

Panseri³

et al. 2019

Recent Advances in Cannabinoid Research

View full text Add to dashboard Cite

show abstract

“…Various HPLC and UPLC methods that have been reported for the analysis of cannabinoids in C. sativa L. plant samples and Cannabis consumer products, e.g. hashish, marijuana and cannabis oils, since the year 2010, are summarised in Tables and , and appraised in the following subsections.…”

Section: Hplc and Uplc (Or Uhplc) Analysis Of Naturally Occurring Canmentioning

confidence: 99%

A review on the recent advances in HPLC, UHPLC and UPLC analyses of naturally occurring cannabinoids (2010–2019)

Nahar

Önder

Sarker

2019

Phytochemical Analysis

View full text Add to dashboard Cite

Introduction Organic molecules that bind to cannabinoid receptors are called cannabinoids, and they have similar pharmacological properties like the plant, Cannabis sativa L. Hyphenated liquid chromatography (LC), incorporating high‐performance liquid chromatography (HPLC) and ultra‐performance liquid chromatography (UPLC, also known as ultrahigh‐performance liquid chromatography, UHPLC), usually coupled to an ultraviolet (UV), UV‐photodiode array (PDA) or mass spectrometry (MS) detector, has become a popular analytical tool for the analysis of naturally occurring cannabinoids in various matrices. Objective To review literature on the use of various LC‐based analytical methods for the analysis of naturally occurring cannabinoids published since 2010. Methodology A comprehensive literature search was performed utilising several databases, like Web of Knowledge, PubMed and Google Scholar, and other relevant published materials including published books. The keywords used, in various combinations, with cannabinoids being present in all combinations, in the search were Cannabis, hemp, cannabinoids, Cannabis sativa, marijuana, analysis, HPLC, UHPLC, UPLC, quantitative, qualitative and quality control. Results Since 2010, several LC methods for the analysis of naturally occurring cannabinoids have been reported. While simple HPLC‐UV or HPLC‐UV‐PDA‐based methods were common in cannabinoids analysis, HPLC‐MS, HPLC‐MS/MS, UPLC (or UHPLC)‐UV‐PDA, UPLC (or UHPLC)‐MS and UPLC (or UHPLC)‐MS/MS, were also used frequently. Applications of mathematical and computational models for optimisation of different protocols were observed, and pre‐analyses included various environmentally friendly extraction protocols. Conclusions LC‐based analysis of naturally occurring cannabinoids has dominated the cannabinoids analysis during the last 10 years, and UPLC and UHPLC methods have been shown to be superior to conventional HPLC methods.

show abstract

“…Additionally, the pre-analytical phase has to be taken into account for method development and validation. Extraction, storage conditions, and stability determination play a pivotal role in the analysis of C. sativa L.-derived products (Dussy et al 2005;Brighenti et al 2017;Mudge et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Determination of cannabinoids in Cannabis sativa L. samples for recreational, medical, and forensic purposes by reversed-phase liquid chromatography-ultraviolet detection

et al. 2018

View full text Add to dashboard Cite

Background: Currently, an increasing demand of cannabis-derived products for recreational and medical use is observed. Therefore, the reliable and fast quantification of cannabinoids in hemp samples is essential for the control of product from Cannabis sativa, L. strains. In general, gas chromatography (GC) is the method of choice for the quantification of cannabinoids whereas this method is time consuming and the detection of acidic precursor is not feasible without derivatization. Methods: We report the successful development and validation of an accurate and broadly applicable reversedphase high-performance liquid chromatography (RP-HPLC) method coupled to an ultra violet (UV) detector including an optimized extraction procedure for the separation and quantification of eight different cannabinoids.Results: The optimized method is able to separate cannabidivarin, cannabidiolic acid, cannabigerolic acid, cannabigerol, cannabidiol, cannabinol, Δ9-tetrahydrocannabinol, and tetrahydrocannabinolic acid within 10 min. For all target analytes, the %-Bias at the lower and upper calibration range varied from − 1.3 to 10.3% and from − 3.9 to 8.6%, respectively. The most suitable agent for extracting cannabis plant samples was evaluated to be a mixture of acetonitrile and water in a ratio 1:1. The extraction efficiency was more than 95% for all analytes in recreational hemp samples. Stability studies on acidic cannabinoids showed a high likeliness of decarboxylation at 100°C and aromatization after exposure to UV light, respectively. A modified loss on drying method revealed a moisture content between 4 and 10%. The developed method was successfully applied to measure the cannabinoid content in recreational and forensic hemp samples representing broad range of cannabinoid amounts and patterns. Conclusion:The present work proposes validated methods for the determination of cannabinoids in cannabis samples. The use of RP-HPLC-UV renders this method broadly applicable and allows the detection of acidic precursor in even less time compared to GC-based methods.

show abstract

Development of a new extraction technique and HPLC method for the analysis of non-psychoactive cannabinoids in fibre-type Cannabis sativa L. (hemp)

Cited by 216 publications

References 25 publications

Quality Traits of Medical Cannabis sativa L. Inflorescences and Derived Products Based on Comprehensive Mass-Spectrometry Analytical Investigation

Quality Traits of Medical Cannabis sativa L. Inflorescences and Derived Products Based on Comprehensive Mass-Spectrometry Analytical Investigation

A review on the recent advances in HPLC, UHPLC and UPLC analyses of naturally occurring cannabinoids (2010–2019)

Determination of cannabinoids in Cannabis sativa L. samples for recreational, medical, and forensic purposes by reversed-phase liquid chromatography-ultraviolet detection

Contact Info

Product

Resources

About