Development of a new microbial toxicity test based on inhibition of alpha-amylase production and its sensitivity against metal contaminants
Abstract:Varying test parameters and procedures were tested and standardized for a new aquatic toxicity assay based on the inhibition of alpha-amylase production by Bacillus subtilis TISTR 1528. Suitable values were determined for the initial cell density and incubation period, as well as the culture medium to improve the new assay's applicability for testing the toxicity of metal pollutants,. The sensitivity and repeatability of the test results were later determined using three reference heavy metals (Zn 2+ , Cu 2+ a… Show more
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