Development of a novel amide-silica stationary phase for the reversed-phase HPLC separation of different classes of phytohormones

Aral, Hayriye; Aral, Tarık; Ziyadanoğulları, Berrin; Ziyadanoğulları, Recep

doi:10.1016/j.talanta.2013.05.007

Cited by 24 publications

(22 citation statements)

References 32 publications

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“…HPLC has been one of the most widely used separation techniques in auxin analysis [111]. HPLC has been used for extract purification both in herbaceous species [112] and forest species (e.g., Populus tremula × P. tremuloides Michx.)…”

Section: Chromatography and Mass Spectrometrymentioning

confidence: 99%

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Stuepp

Wendling

Trueman

et al. 2017

Forests

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Qualitative and quantitative hormone analyses have been essential for understanding the metabolic, physiological, and morphological processes that are influenced by plant hormones. Auxins are key hormones in the control of many aspects of plant growth and development and their endogenous levels are considered critical in the process of adventitious root induction. Exogenous auxins are used extensively in the clonal propagation of tree species by cuttings or tissue culture. Understanding of auxin effects has advanced with the development of increasingly accurate methods for auxin quantification. However, auxin analysis has been challenging because auxins typically occur at low concentrations, while compounds that interfere with their detection often occur at high concentrations, in plant tissues. Interference from other compounds has been addressed by extensive purification of plant extracts prior to auxin analysis, although this means that quantification methods have been limited by their expense. This review explores the extraction, purification, and quantification of auxins and the application of these techniques in developing improved methods for the clonal propagation of forestry trees.

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Section: Chromatography and Mass Spectrometrymentioning

confidence: 99%

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Stuepp

Wendling

Trueman

et al. 2017

Forests

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“…HPLC is one of the most important analytical techniques for qualitative and quantitative determinations with its efficiency, excellent reproducibility, and wide range applicability . The performance of this technique depends on several parameters, but the most important of them is the stationary phase .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of new silica‐based humic acid stationary phase for the separation of tocopherols in cold‐pressed oils by normal‐phase high‐performance liquid chromatography

Ayyıldız

2015

J of Separation Science

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A new humic acid stationary phase was prepared by immobilizing humic acid onto aminopropyl silica via an amide linkage formation and used, for the first time, for the separation and quantification of the tocopherol compounds in cold-pressed oil samples under normal-phase high-performance liquid chromatography conditions. Parameters affecting the chromatographic separation such as mobile phase composition and flow rate were optimized. By evaluating the calculations of capacity factor, asymmetry factor, resolution, selectivity factor, and theoretical plate number, the best separation was obtained with isocratic elution of n-hexane and isopropyl alcohol (99:1% v/v) at a flow rate of 1.0 mL/min. The effluent was monitored by a fluorescence detector set at excitation and emission wavelengths 295 and 330 nm, respectively. All compounds were separated in 20 min. The method was validated according to international guidelines and found to be linear in a wide concentration range, also the mean recovery of the compounds ranged from 97.9 to 99.2%, with a CV less than 2.7% in all cases. The results showed that the developed stationary phase is suitable for the separation and quantification of the tocopherol compounds in real oil samples.

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“…Based on the results of the chromatographic separation under the condition in Section 2.5 for Dolan/Synder method, k of each solute was calculated, then α was calculated with ethylbenzene by Eq. (2). Based on the data of α, the column parameters were calculated by multiple regressions and listed in Table 3.…”

Section: Investigation Of the Column Parametersmentioning

confidence: 99%

Synthesis and chromatographic evaluation of phenyl/tetrazole bonded stationary phase based on thiol‐epoxy ring opening reaction

Jin

Liu

Yang

et al. 2018

J of Separation Science

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A silica-based reversed-phase stationary phase bonding with phenyl and tetrazole groups was synthesized by thiol-epoxy ring opening reaction. The bonded groups could not only provide hydrophobic interaction, but also π-π, hydrogen bonding, electrostatic interactions, and so on. The results of characterization with elemental analysis and solid-state C cross-polarization magic-angle-spinning NMR spectroscopy indicated the successful preparation of phenyl/tetrazole sulfoether bonded stationary phase. Chromatographic evaluation revealed that phenyl/tetrazole sulfoether bonded stationary phase behaved well under the reversed-phase mode. The column parameters (H, S*, A, B, and C) showed different selectivity compared with some typical commercial columns, and it was validated by the separation of estrogen, ginsenoside, alkaloid samples. Based on the different selectivity between phenyl/tetrazole sulfoether bonded stationary phase and C18 columns, phenyl/tetrazole sulfoether bonded stationary phase also showed potential to construct a 2D reversed-phase liquid chromatography system with C18. And it was verified by the separation of corydalis tuber and curcuma zedoary extracts.

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Development of a novel amide-silica stationary phase for the reversed-phase HPLC separation of different classes of phytohormones

Cited by 24 publications

References 32 publications

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Evaluation of new silica‐based humic acid stationary phase for the separation of tocopherols in cold‐pressed oils by normal‐phase high‐performance liquid chromatography

Synthesis and chromatographic evaluation of phenyl/tetrazole bonded stationary phase based on thiol‐epoxy ring opening reaction

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