Development of a Novel and Stable Indicating RP-HPLC Method for the Simultaneous Analysis of 12 Impurities in Midazolam and Midazolam Injection Products

Chen, Dandan; Shen, Dandan; Huang, Li; Hou, Lei; Xiong, Yingfei; Zeng, Linggao; Wang, Jianhua

doi:10.1007/s10337-022-04193-5

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…The objective of this study was to explore the separation mechanisms involved in the chromatographic separation of small polar compounds under HILIC conditions and to assess their lipophilicity. As model compounds, nine small, relatively polar drugs were chosen which belong to classes of neuromuscular blocking agents usually administered during anesthesia (Pancuromium, Esmeron, Midarine, and Atracurium) [24, 25], anesthetics, intravenous (Etomidate) and local (Lidocaine) [26], opioid analgesics (Fentanyl and Rapifen) [27], and a class of sedative‐hypnotic drugs (Midazolam) [28].…”

Section: Introductionmentioning

confidence: 99%

Exploring separation mechanisms and lipophilicity in hydrophilic interaction chromatography conditions by thin‐layer chromatography of anesthetics and adjuvant drugs as polar model compounds

Radoičić,

Šegan,

Milojković‐Opsenica

2024

J of Separation Science

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The chromatographic behavior of the selected compounds was studied under conditions of hydrophilic interaction liquid chromatography (HILIC). The effect of mobile phase composition on the retention in different chromatographic systems was systematically examined using high‐performance thin‐layer chromatography. The sorbents of different polarity and adsorption characteristics were selected and mixtures of water and organic solvents of various compositions, from pure water to pure organic solvent were used as mobile phases. Increasing the amount of water in the mobile phase leads to a conversion of the separation mechanism, and the retention curves have a characteristic “U” shape. The conversion between the adsorption and partition mechanisms is most likely continuous and depends on the chemical nature of separated substances, the stationary phase as well as on organic component of the mobile phase. Silica gel can be considered the most suitable stationary phase for the systematic investigation of the chromatographic behavior of the test compounds, whereas acetonitrile was the most suitable solvent. The obtained results contribute to the understanding of the dominant separation mechanism, the type, and the intensity of the interactions between separated substances with both stationary and mobile phases. Besides, the lipophilicity parameters obtained under HILIC conditions were evaluated and correlated with the calculated values.

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Section: Introductionmentioning

confidence: 99%

Exploring separation mechanisms and lipophilicity in hydrophilic interaction chromatography conditions by thin‐layer chromatography of anesthetics and adjuvant drugs as polar model compounds

Radoičić,

Šegan,

Milojković‐Opsenica

2024

J of Separation Science

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Development and application of a high-performance liquid chromatography diode-array detection (HPLC–DAD) method for the simultaneous quantification of phenolic compounds in the aerial part of Glehnia littoralis

Park,

Choi,

et al. 2024

Appl Biol Chem

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Glehnia littoralis, a medicinal herb employed in traditional practices for alleviating fatigue, cough, and a dry throat, is recognized for its beneficial properties due to a diverse array of active compounds found in its extracts. For example, the G. littoralis roots (Radix Glehniae) mainly contain coumarins and phenolic acids, serving as the primary focus of this study. Despite the widespread use of the tools in various industries and the development of multiple analytical methods for their examination, the edible aerial parts have industrial potential, and there is currently no analytical method available to identify their key components. In this study, a high-performance liquid chromatography method combined with diode array detection (HPLC–DAD) was developed to simultaneously detect 16 phenolic compounds previously reported to be present in the edible aerial parts of G. littoralis. The proposed approach included using gradient elution to change the solvent system from water/acetonitrile to water/methanol. Furthermore, the method validation was conducted, assessing its linearity, limit of detection, limit of quantification, precision, accuracy, and recovery, all of which demonstrated satisfactory results. Subsequently, the developed method was applied to quantify the phenolic compounds in various G. littoralis samples obtained from different organs, solvent extraction processes, and processing methods. Moreover, the online HPLC-ABTS (2,2ʹ-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assay was used to evaluate the antioxidant capacities of individual constituents, identifying four important antioxidants and estimate the overall antioxidant capacity of the G. littoralis extract.

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Development of a Novel and Stable Indicating RP-HPLC Method for the Simultaneous Analysis of 12 Impurities in Midazolam and Midazolam Injection Products

Cited by 2 publications

References 17 publications

Exploring separation mechanisms and lipophilicity in hydrophilic interaction chromatography conditions by thin‐layer chromatography of anesthetics and adjuvant drugs as polar model compounds

Exploring separation mechanisms and lipophilicity in hydrophilic interaction chromatography conditions by thin‐layer chromatography of anesthetics and adjuvant drugs as polar model compounds

Development and application of a high-performance liquid chromatography diode-array detection (HPLC–DAD) method for the simultaneous quantification of phenolic compounds in the aerial part of Glehnia littoralis

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