Development of a novel compact sonicator for cell disruption

Borthwick, K.A.J.; Coakley, W. Terence; McDonnell, Martin B.; Nowotny, H.; Benes, E.; Gröschl, Martin

doi:10.1016/j.mimet.2004.09.012

Cited by 74 publications

(55 citation statements)

References 11 publications

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“…Spore disruption, spore death, and increases in amplifiable DNA were demonstrated using B. globigii spore preparations. 4 Borthwick et al 3,8 described a flow-through sonicator using a tubular piezoceramic transducer at 267 kHz. In experiments with B. subtilis var.…”

Section: Discussionmentioning

confidence: 99%

A Flow-Through Ultrasonic Lysis Module for the Disruption of Bacterial Spores

Warner

Bruckner-Lea

Grate

et al. 2009

JALA: Journal of the Association for Laboratory Automation

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An automated, flow-through ultrasonic lysis module that is capable of disrupting bacterial spores to increase the DNA available for biodetection is described. The system uses a flow-through chamber that allows for direct injection of the sample without the need for a chemical or enzymatic pretreatment step to disrupt the spore coat before lysis. Lysis of Bacillus subtilis spores, a benign simulant of Bacillus anthracis, is achieved by flowing the sample through a tube whose axis is parallel to the faces of two transducers that deliver 10 W cm−2 to the surface of the tube at 1.4-MHz frequency. Increases in amplifiable DNA were assessed by real-time PCR analysis that showed at least a 25-fold increase in amplifiable DNA after ultrasonic treatment with glass beads, compared with controls with no ultrasonic power applied. The ultrasonic system and integrated fluidics are designed as a module that could be incorporated into multistep, automated sample treatment and detection systems for pathogens.

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Section: Discussionmentioning

confidence: 99%

A Flow-Through Ultrasonic Lysis Module for the Disruption of Bacterial Spores

Warner

Bruckner-Lea

Grate

et al. 2009

JALA: Journal of the Association for Laboratory Automation

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“…The kinetics of microbial inactivation are first order or pseudo first order Tsukamoto et al 2004;Zhang et al 2006) with (Guerrero et al 2001;Furuta et al 2004;Tsukamoto et al 2004) and frequency (Borthwick et al 2005). Tsukamoto et al (2004) showed that the rate of inactivation of Saccharomyces cerevisiae yeast cells by ultrasonic irradiation was a function of the amplitude of the ultrasonic wave and the initial cell numbers and was highest at higher amplitudes and lower initial cell numbers.…”

Section: Effects On Microorganismsmentioning

confidence: 99%

“…Tsukamoto et al (2004) showed that the rate of inactivation of Saccharomyces cerevisiae yeast cells by ultrasonic irradiation was a function of the amplitude of the ultrasonic wave and the initial cell numbers and was highest at higher amplitudes and lower initial cell numbers. Subsequently, Borthwick et al (2005) reported that yeast cell disruption was greater in a novel compact 267 kHz sonicator than in a lower frequency 20 kHz probe sonicator at the same exposure time.…”

Section: Effects On Microorganismsmentioning

confidence: 99%

High power ultrasonics as a novel tool offering new opportunities for managing wine microbiology

Jiranek

Grbin

Yap³

et al. 2007

Biotechnol Lett

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Industrial scale food and beverage processes that utilize microorganisms are typically faced with issues related to the exclusion, suppression or elimination of spoilage organisms. Yet the use of traditional anti-microbial treatments such as heat, chemical biocides or sterile filtration may themselves be restricted by regulations or else be undesirable due to their adverse sensory impacts on the product. High power ultrasound (HPU) is a technology whose application has been evaluated if not exploited in several food and beverage processes but has yet to be introduced into the wine industry. This review examines the research findings from related industries and highlights possible applications and likely benefits of the use of HPU in winemaking.

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“…The ultrasonic characterization for non-destructive testing and imaging usually employs a low power signal with frequency up to 70 MHz. By contrast, the power of order of 10 W is generally required in ultrasonic cleaning (Saikia et al, 2014) dyeing (Guesmi et al, 2013), food processing (Kentish, Feng, 2014), natural product extraction (Esclapez et al, 2011), microbial cell disruption (Borthwick et al, 2005), acoustic cavitation (Khmelev et al, 2015) and sonochemistry (Son et al, 2012). Moreover, applications of power exceeding 100 W are common in biodegradation (Zawieja, Wolny, 2011) and alloy treatment (Shao et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Electrical Characterization of 65 W Cubic Sonoreactor with Horizontally Stacked Transducers

Kerdthongmee¹,

Pholnak²,

Sirisathitkul³

et al. 2017

Archives of Acoustics

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A sonoreactor was assembled with stacked lead zirconate titanate transducers. These transducers were attached on one side of a 10 × 10 × 10 cm 3 chamber and driven by an integrated circuit power amplifier. The impedance of the reactor was analyzed in order to determine a matching inductance. The electrical frequency could be varied from 20 to 50 kHz and the electrical output power was adjustable up to 65 W. The highest power was obtained in the case of resonance at 31 kHz and the maximum temperature at the heat sink of the amplifier rose to 42.0 • C. Both acoustic cavitation and mechanical effects could be utilized in this sonoreactor for a variety of purposes including sonochemical synthesis, ultrasonic cleaning and microbial cell disruption.

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Development of a novel compact sonicator for cell disruption

Cited by 74 publications

References 11 publications

A Flow-Through Ultrasonic Lysis Module for the Disruption of Bacterial Spores

A Flow-Through Ultrasonic Lysis Module for the Disruption of Bacterial Spores

High power ultrasonics as a novel tool offering new opportunities for managing wine microbiology

Electrical Characterization of 65 W Cubic Sonoreactor with Horizontally Stacked Transducers

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