Development of a Novel Electrochemical Inhibition Sensor Array Based on Bacteria Immobilized on Modified Screen-Printed Gold Electrodes for Water Pollution Detection

Abstract

The development of a novel and simple inhibition biosensor array for detection of water pollutants based on immobilized bacteria is the main goal of this work. A series of electrochemical measurements (i.e. cyclic voltammograms) were carried out on screen-printed gold electrodes with three types of bacteria, namely Escherichia coli, Shewanella oneidensis, and Methylococcus capsulatus, immobilized via poly L-lysine. For comparison purposes, similar measurements were carried out on bacteria samples in solutions,; also optical measurements (fluorescence microscopy, optical density, and flow cytometry) were performed on the same bacteria in both liquid and immobilized forms. The study of the effect of heavy metal ions (lead), pesticides (atrazine) and petrochemicals (hexane) on DC electrochemical characteristics of immobilized bacteria revealed a possibility of pattern recognition of the above inhibition agents in aquatic environment.

Platinum Nanoparticle in Tantalum Electrode for the Electrochemical Analysis of Heavy Metal Ions Formed by the Ion Beam Sputtering Deposition

“…The most widely used material in electrochemical measurement of heavy metals , particularly nanomaterial-based metals, metal oxides, carbonated metals and their composites [17]. The specific feature of the Ion beam sputtering (IBSD) process is the capacity to deposit high density films at a low substratum temperature through the dynamics for atomic or molecular transport growth mechanism, which gives it numerous benefits including excellent stoichiometry movement, strong durability and simple set-up for the creation of films [18][19][20]. The platinum nanoelectrode was rendered in tantalum surface by IBSD and a novel electrode was then used for electrochemical study for detecting of heavy metal ions (Hg 2+ , Cu 2+ and Ag 2+ ) in the different mediums.…”