Development of a novel electrochemical DNA biosensor based on elongated hexagonal-pyramid CdS and poly-isonicotinic acid composite film

Zheng, Delun; Wang, Qingxiang; Gao, Feng; Wang, Qinghua; Qiu, Weiwei; Gao, Fei

doi:10.1016/j.bios.2014.04.011

Cited by 23 publications

(8 citation statements)

References 30 publications

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“…As an alternative to the traditional methods such as fluorescence in - situ hybridization 6 , flow cytometry 7 and real-time quantitative reverse transcription PCR 8 , the electrochemical approach receives increasing attention since it has predominant advantages of low cost, easy operation, excellent compatibility with micro-fabrication 9 10 . Most of the developed DNA electrochemical biosensors were fabricated based on the linear DNA probes and the external hybridization indicators 11 12 13 . Notwithstanding, these biosensors have high sensitivity to target DNA, the low selectivity, large background response from the non-specifically bound indicators, and complicated indicators binding/rinsing process upon each hybridization step limit their practical application.…”

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confidence: 99%

Facile construction of a highly sensitive DNA biosensor by in-situ assembly of electro-active tags on hairpin-structured probe fragment

Wang

Gao

et al. 2016

Sci Rep

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An ultrasensitive DNA biosensor has been developed through in-situ labeling of electroactive melamine-Cu2+ complex (Mel-Cu2+) on the end of hairpin-like probe using gold nanoparticles (AuNPs) as the signal amplification platform. The 3′-thiolated hairpin-like probe was first immobilized to the gold electrode surface by the Au-S bond. The AuNPs were then tethered on the free 5′-end of the immobilized probe via the special affinity between Au and the modified -NH2. Followed by, the Mel and Cu2+ were assembled on the AuNPs surface through Au-N bond and Cu2+-N bond, respectively. Due to the surface area and electrocatalytic effects of the AuNPs, the loading amount and electron transfer kinetic of the Mel-Cu2+ were enhanced greatly, resulting in significantly enhanced electrochemical response of the developed biosensor. Compared with the synthesis process of conventional electroactive probe DNA accomplished by homogeneous method, the method presented in this work is more reagent- and time-saving. The proposed biosensor showed high selectivity, wide linear range and low detection limit. This novel strategy could also be extended to the other bioanalysis platforms such as immunosensors and aptasensors.

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confidence: 99%

Facile construction of a highly sensitive DNA biosensor by in-situ assembly of electro-active tags on hairpin-structured probe fragment

Wang

Gao

et al. 2016

Sci Rep

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“…Many complex EBs involving several biological reactions and washing steps have been described in the literature [9][10][11]. Such procedures differ from the ideal concepts of a POC device.…”

Section: Introductionmentioning

confidence: 98%

Towards a blocking-free electrochemical immunosensing strategy for anti-transglutaminase antibodies using screen-printed electrodes

Martín‐Yerga

Costa-Garcı́a

2015

Bioelectrochemistry

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“…Furthermore, genosensors that rely on electrochemical impedance spectroscopy require a long data-acquisition time [ 29 ]. EIS has been successfully replaced by other less sophisticated electrochemical techniques [ 30 ], such as differential pulse voltammetry [ 31 ], chronoamperometry [ 31 , 32 , 33 ], chronopotentiometry [ 34 ], or switching DNA [ 35 ]. A structure-switching probe operates via the alteration of distance of the redox labels from the electrode caused by target-induced structure switching, representing a significant advance of using minimal reagents with working steps, a simplified setup, cost efficiency, high sensitivity, and excellent compatibility with miniaturization potential [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Resistive DNA Biosensor for the Detection of HPV Type 16

et al. 2021

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In this work, a low-cost and rapid electrochemical resistive DNA biosensor based on the current relaxation method is described. A DNA probe, complementary to the specific human papillomavirus type 16 (HPV-16) sequence, was immobilized onto a screen-printed gold electrode. DNA hybridization was detected by applying a potential step of 30 mV to the system, composed of an external capacitor and the modified electrode DNA/gold, for 750 µs and then relaxed back to the OCP, at which point the voltage and current discharging curves are registered for 25 ms. From the discharging curves, the potential and current relaxation were evaluated, and by using Ohm’s law, the charge transfer resistance through the DNA-modified electrode was calculated. The presence of a complementary sequence was detected by the change in resistance when the ssDNA is transformed in dsDNA due to the hybridization event. The target DNA concentration was detected in the range of 5 to 20 nM. The results showed a good fit to the regression equation ΔRtotal(Ω)=2.99 × [DNA]+81.55, and a detection limit of 2.39 nM was obtained. As the sensing approach uses a direct current, the electronic architecture of the biosensor is simple and allows for the separation of faradic and nonfaradaic contributions. The simple electrochemical resistive biosensor reported here is a good candidate for the point-of-care diagnosis of HPV at a low cost and in a short detection time.

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Development of a novel electrochemical DNA biosensor based on elongated hexagonal-pyramid CdS and poly-isonicotinic acid composite film

Cited by 23 publications

References 30 publications

Facile construction of a highly sensitive DNA biosensor by in-situ assembly of electro-active tags on hairpin-structured probe fragment

Facile construction of a highly sensitive DNA biosensor by in-situ assembly of electro-active tags on hairpin-structured probe fragment

Towards a blocking-free electrochemical immunosensing strategy for anti-transglutaminase antibodies using screen-printed electrodes

Electrochemical Resistive DNA Biosensor for the Detection of HPV Type 16

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