Development of a novel glucose enzyme fuel cell system employing protein engineered PQQ glucose dehydrogenase

Yuhashi, Noriko; Tomiyama, Masamitsu; Okuda, Junko; Igarashi, Satoshi; Sode, Koji

doi:10.1016/j.bios.2004.08.017

Cited by 115 publications

(71 citation statements)

References 14 publications

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“…Interference from other sugars (i.e., lactose, galactose, maltose, cellobiose, and xylose) and common alcohols has been reported. 122,123 Biosensors utilizing direct electro-oxidation of glucose (E-2-a configuration shown in Figure 1B) also lack specificity and are susceptible to interferences from other sugars.…”

Section: Selectivity Of Sensormentioning

confidence: 99%

Technologies for Continuous Glucose Monitoring: Current Problems and Future Promises

Vaddiraju

Burgess

Tomazos

et al. 2010

J Diabetes Sci Technol

230

224

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Devices for continuous glucose monitoring (CGM) are currently a major focus of research in the area of diabetes management. It is envisioned that such devices will have the ability to alert a diabetes patient (or the parent or medical care giver of a diabetes patient) of impending hypoglycemic/hyperglycemic events and thereby enable the patient to avoid extreme hypoglycemic/hyperglycemic excursions as well as minimize deviations outside the normal glucose range, thus preventing both life-threatening events and the debilitating complications associated with diabetes. It is anticipated that CGM devices will utilize constant feedback of analytical information from a glucose sensor to activate an insulin delivery pump, thereby ultimately realizing the concept of an artificial pancreas. Depending on whether the CGM device penetrates/breaks the skin and/or the sample is measured extracorporeally, these devices can be categorized as totally invasive, minimally invasive, and noninvasive. In addition, CGM devices are further classified according to the transduction mechanisms used for glucose sensing (i.e., electrochemical, optical, and piezoelectric). However, at present, most of these technologies are plagued by a variety of issues that affect their accuracy and long-term performance. This article presents a critical comparison of existing CGM technologies, highlighting critical issues of device accuracy, foreign body response, calibration, and miniaturization. An outlook on future developments with an emphasis on long-term reliability and performance is also presented.

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Section: Selectivity Of Sensormentioning

confidence: 99%

Technologies for Continuous Glucose Monitoring: Current Problems and Future Promises

Vaddiraju

Burgess

Tomazos

et al. 2010

J Diabetes Sci Technol

230

224

View full text Add to dashboard Cite

show abstract

“…Generally, glucose biosensors and GBFCs can be classified into two categories according to the types of catalysts, namely enzymebased and nonenzyme-based ones [2][3][4]. Research findings demonstrate that enzymatic catalysts exhibit high activity and excellent selectivity, but the unstable operating environment and fragile stability greatly hinder their practical applications [5,6]. To overcome these drawbacks, non-enzymatic catalysts (such as precious metal nanoparticles or alloys [7][8][9][10], composite materials [11,12], polymers [13,14] and transition metal oxides [15][16][17][18]) as enzyme mimics show higher performance than enzymatic ones.…”

Section: Introductionmentioning

confidence: 99%

Rectangular flake-like mesoporous NiCo2O4 as enzyme mimic for glucose biosensing and biofuel cell

et al. 2017

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“…One of the main challenges in commercializing EFCs consists in short lifetime which is related to enzymes poor stability. To extend the lifetime of enzyme electrodes several strategies have been proposed, ranging from genetic engineering methods to alter enzyme amino acid sequence [12,13], and immobilization techniques in external matrices [14,15], which generally result in catalyst stabilization against thermal and chemical denaturation.…”

Section: Introductionmentioning

confidence: 99%

Development of glucose oxidase-based bioanodes for enzyme fuel cell applications

et al. 2012

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We fabricated an enzyme fuel cell (EFC) device based on glucose as fuel and glucose oxidase (GOx) as biocatalyst. As a strategy to improve GOx stability, preserving at the same time the enzyme catalytic activity, we propose an immobilization procedure to entrap GOx in a polymer matrix based on Nafion and multiwalled carbon nanotubes. Circular dichroism (CD) spectra were recorded to study changes in the 3D structure of GOx that might be generated by the immobilization procedure. The comparison between the CD features of GOx immobilized and free in solution indicates that the shape of the spectra and position of peaks do not significantly change. The bioelectrocatalytic activity toward glucose oxidation of immobilized GOx was studied by cyclic voltammetry and chronoamperometry experiments. Such electrochemical experiments allow monitoring the rate of GOx-catalyzed glucose oxidation and extrapolating GOx kinetic parameters. Results demonstrate that immobilized GOx has high catalytic efficiency, due the maintaining of regular and well-ordered structure of the immobilized enzyme, as indicated by spectroscopic findings. Once investigated the electrode structure-property relationship, an EFC device was assembled using the GOx-based bioanode, and sulfonated poly ether ether ketone as electrolyte membrane.Polarization and power density curves of the complete EFC device were acquired, demonstrating the suitability of the immobilization strategy and materials to be used in EFCs.

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Development of a novel glucose enzyme fuel cell system employing protein engineered PQQ glucose dehydrogenase

Cited by 115 publications

References 14 publications

Technologies for Continuous Glucose Monitoring: Current Problems and Future Promises

Technologies for Continuous Glucose Monitoring: Current Problems and Future Promises

Rectangular flake-like mesoporous NiCo2O4 as enzyme mimic for glucose biosensing and biofuel cell

Development of glucose oxidase-based bioanodes for enzyme fuel cell applications

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