Development of a novel HPTLC-based method for the simultaneous quantification of clinically relevant lipids from cells and tissue extracts

Abstract

Lipids such as cholesterol, triglycerides, and fatty acids play important roles in the regulation of cellular metabolism and cellular signaling pathways and, as a consequence, in the development of various diseases. It is therefore important to understand how their metabolism is regulated to better define the components involved in the development of various human diseases. In the present work, we described the development and validation of an HPTLC method allowing the separation and quantification of free cholesterol, cholesteryl esters, nonesterified fatty acids, and triglycerides. This method will be of interest as the quantification of these lipids in one single assay is difficult to perform.

Apolipoprotein-mediated regulation of lipid metabolism induces distinctive effects in different types of breast cancer cells

“…For lipid extractions, lipids from the cell pellets were extracted using the method of Bligh and Dyer [13]. Free and esterified cholesterol quantification was performed by HPTLC as previously described [14]. Cellular cholesterol levels were calculated by dividing the cholesterol content by the total protein content.…”

Tissue cholesterol metabolism and prostate cancer aggressiveness: Ethno‐geographic variations

“…20 High-performance thin-layer chromatography (HPTLC) was then performed, as previously described. 21 For the development, the mobile phase used was a mixture of hexane: diethyl ether: acetic acid (70: 30: 1, v/v/v).…”