Development of a novel multiplex polymerase chain reaction system for forensic individual identification using insertion/deletion polymorphisms

Abstract

Insertion/deletion (InDel) polymorphisms have been widely used in the fields of population genetics, genetic map constructions, and forensic investigations owing to the advantages of their low mutation rates, widespread distributions in the human genome, and small amplicon sizes. In order to provide more InDels with high discrimination power in Chinese populations, we selected and constructed one novel multiplex PCR‐InDel panel for forensic individual identification. Genetic distributions of these 35 InDels in five reference populations from East Asia showed low genetic differentiations among these populations. Forensic efficiency evaluations of these InDels revealed that these loci could perform well for forensic individual identifications in these reference populations. In the meantime, genetic diversities and forensic parameters of these InDels were further investigated in the studied Kazak group. Mean value of polymorphism information content for 35 InDels was 0.3611. Cumulative power of discrimination of 35 InDels was 0.99999999999999603 in Kazak group. Given these results, the panel is suitable for individual identifications in the studied Kazak and these reference populations.

Genetic Polymorphisms and Forensic Efficiencies of a Set of Novel Autosomal InDel Markers in a Chinese Mongolian Group

“…Genotyping. In this study, the PCR amplification of 35 InDel loci was conducted on a GeneAmp PCR system 9700 thermal cycler (Applied Biosystems, Foster City, CA, USA), following previous descriptions [15]. en, the PCR amplification products were separated and detected by capillary electrophoresis on the ABI 3500 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA).…”

“…e reference populations of this study were the intercontinental populations [16] and Kazak group [15] reported before, which were African (7 populations), American (4 populations), East Asian (5 populations), European (5 populations), South Asian (5 populations), and Chinese Kazak group. Detailed information of these continental populations are as follows: African populations include ACB, ASW, ESN, GWD, LWK, MSL, and YRI; American populations include CLM, MXL, PEL, and PUR; East Asian populations include CDX, CHB, CHS, JPT, and KHV; European populations include CEU, FIN, GBR, IBS, and TSI; South Asian populations include GIH, ITU, PJL, STU, and BEB.…”

“…Previously, we developed a novel multiplex PCR-InDel panel for forensic individual identifications in the Chinese Kazak group and reference populations from East Asia [15]. Here, genetic distributions and forensic efficiencies of these InDels in the Chinese Mongolian group were further investigated.…”

Developing and population analysis of a new multiplex panel of 18 microhaplotypes and compound markers using next generation sequencing and its application in the Shaanxi Han population

“…More importantly, DNA genotypes of DIP loci can be easily detected by capillary electrophoresis. Recently, forensic researchers developed some DIP panels for human identifications [15,16] or ancestry analyses [17,18]. To further enhance the discrimination power of DIP loci, Wendt et al assessed genetic variations in the flanking regions of 68 DIP loci by using the next generation sequencing (NGS) and found that these adjacent variations could improve forensic efficiency of the single DIP locus [14].…”

“…In this study, we searched potential polymorphic markers (SNP or DIP) in neighboring regions of previously reported SNPs and DIPs [5,[8][9][10][11]15]. Firstly, 23 novel genetic makers, including 10 microhaplotypes and 13 compound markers, were initially selected for forensic applications.…”