Development of a novel one-step triplex PCR assay for the simultaneous detection of porcine circovirus type 2, porcine parvovirus and classical swine fever virus in a single tube

Rajkhowa, S.; Choudhury, Minati; Pegu, Seema Rani; Sarma, D. K.; Gupta, Vivek Kumar

doi:10.1111/lam.13732

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…Swine pathogens, including viruses, bacteria, parasites, mycoplasma, etc., seriously endanger the development of the pig industry. Swine viruses, such as transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), Influenza A virus (IAV), African swine fever virus (ASFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus (PCV), and pseudorabies virus (PRV), cause severe diseases through single infection and/or coinfection [1][2][3][4][5]. Furthermore, viruses, such as PCV and PRRSV, are usually the primary pathogens that break through the host's defense, which generally leads to immunosuppression and causes secondary infection of other pig pathogens [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Flavonoids as Potential Antiviral Agents for Porcine Viruses

Zhang

Chen

et al. 2022

Pharmaceutics

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Flavonoids are types of natural substances with phenolic structures isolated from a variety of plants. Flavonoids have antioxidant, anti-inflammatory, anticancer, and antiviral activities. Although most of the research or applications of flavonoids are focused on human diseases, flavonoids also show potential applicability against porcine virus infection. This review focuses on the recent progress in antiviral mechanisms of potential flavonoids against the most common porcine viruses. The mechanism discussed in this paper may provide a theoretical basis for drug screening and application of natural flavonoid compounds and flavonoid-containing herbs to control porcine virus infection and guide the research and development of pig feed additives.

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Section: Introductionmentioning

confidence: 99%

Flavonoids as Potential Antiviral Agents for Porcine Viruses

Zhang

Chen

et al. 2022

Pharmaceutics

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Coinfection of porcine astrovirus and other porcine viruses in diarrheic pigs in Haryana, India

Vaishali,

Gupta,

Kumar

et al. 2023

Arch Virol

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In this study, 306 rectal swabs from diarrheal pigs of various ages (0-3 weeks, 3-6 weeks, and > 6 weeks) were collected from 54 piggery units in different climatic zones in Haryana, India. These samples were tested for the presence of porcine astrovirus (PAstV), porcine circovirus 2 (PCV-2), porcine rotavirus A (PRV-A), and classical swine fever virus (CSFv) by reverse transcription-polymerase chain reaction (RT-PCR). Out of the 306 samples tested, 153 (50%), 108 (35.3%), 32 (10.6%), and 3 (0.9%) were detected positive for PAstV, PCV-2, PRV-A, and CSFv, respectively. Mixed infections were found in 77 samples, whereas 135 had only one virus, and 70 and 7 samples had two and more than 2 viruses, respectively. PAstV was more prevalent (55.31%) in pigs aged 3-6 weeks. PCV-2 was more predominant (36.53%) in pigs aged 0-3 weeks, whereas PRV-A was more common in pigs aged 3-6 weeks (11.3%). CSFv was observed in the age group of 0-3 weeks (1.92%). Phylogenetic analysis revealed the circulation of lineage − 2 and − 4 of PAstV in this region. Thus, it can be concluded that the one or more than one virus is circulating in piggery units in Haryana, India.

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Co-infection of porcine astrovirus and other porcine viruses in diarrheic pigs in Haryana, India

Vaishali

Gupta

Kumar

et al. 2023

Preprint

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In this study, 306 rectal swabs from diarrheal pigs of various ages (0–3 weeks, 3–6 weeks, and > 6 weeks) were collected from 54 piggery units in different climatic zones in Haryana, India. These samples were tested for the presence of porcine astrovirus (PAstV), porcine circovirus 2 (PCV-2), porcine rotavirus A (PRV-A), and classical swine fever virus (CSFv) by reverse transcription-polymerase chain reaction (RT-PCR). Out of the 306 samples tested, 153 (50%), 108 (35.3%), 32 (10.6%), and 3 (0.9%) were detected positive for PAstV, PCV-2, PRV-A, and CSFv, respectively. Mixed infections were found in 77 samples, whereas 135 had only one virus, and 70 and 7 samples had two and more than 2 viruses, respectively. PAstV was more prevalent (55.31%) in pigs aged 3–6 weeks. PCV-2 was more predominant (36.53%) in pigs aged 0–3 weeks, whereas PRV-A was more common in pigs aged 3–6 weeks (11.3%). CSFv was observed in the age group of 0–3 weeks (1.92%). Phylogenetic analysis revealed the circulation of lineage − 2 and − 4 of PAstV in this region. Thus, it can be concluded that the one or more than one virus is circulating in piggery units in Haryana, India.

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Development of a novel one-step triplex PCR assay for the simultaneous detection of porcine circovirus type 2, porcine parvovirus and classical swine fever virus in a single tube

Cited by 3 publications

References 20 publications

Flavonoids as Potential Antiviral Agents for Porcine Viruses

Flavonoids as Potential Antiviral Agents for Porcine Viruses

Coinfection of porcine astrovirus and other porcine viruses in diarrheic pigs in Haryana, India

Co-infection of porcine astrovirus and other porcine viruses in diarrheic pigs in Haryana, India

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