Development of a Novel PCR Primer to Differentiate and Identify<i>Bacillus subtilis</i>and Closely Related Species Isolated from Thai Fermented Foods

Kaewklom, Siriporn; Chueakhalm, W.; Suthirawut, Surang; Aunpad, Ratchaneewan

doi:10.1080/08905436.2014.964252

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…(both members of the Bacillus cereus clade). This clearly exemplifies the challenges associated with the identification of members of the Bacillaceae family at species level 39,40 …”

Section: Discussionmentioning

confidence: 83%

“…This clearly exemplifies the challenges associated with the identification of members of the Bacillaceae family at species level. 39,40 The ability of cell-free culture extract to inhibit X. fastidiosa and V. dahlia growth strongly suggests the secretion of antimicrobial compounds into the culture medium. The inhibitory effect was, however, consistently weaker than observed for bacterial suspensions.…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial profile of the culturable olive sporobiota and its potential as a source of biocontrol agents for major phytopathogens in olive agriculture

Manetsberger,

Caballero Gómez,

Benomar

et al. 2023

Pest Management Science

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BACKGROUNDThe phytopathogens Xylella fastidiosa and Verticillium dahliae present an unparalleled threat to olive agriculture. However, there is no efficient field treatment available today for either pest. Spore‐forming bacteria (i.e., the sporobiota) are known for their extraordinary resistance properties and antimicrobial activity. The aim of this study was thus to identify potential novel sustainable spore‐forming biocontrol agents derived from the culturable olive microbiome, termed the sporobiota, in general and in particular against X. fastidiosa and V. dahliae.RESULTSWe demonstrate the wide‐ranging antimicrobial profile of 415 isolates from the culturable olive sporobiota towards human and plant pathogens. We further identified five candidates with antagonistic activity against X. fastidiosa and V. dahliae. These belong to the Bacillus subtilis, Bacillus cereus and Peribacillus simplex clade. The activity was related to the species and their relative origin (soil versus leaf endophytic). It is of particular interest that two of these candidates are already naturally present at the site of disease‐development that is, plant interior. We further confirmed the presence of lipopeptide genes potentially associated with the reported bioactivity.CONCLUSIONSThe study provides insights into how members of the olive sporobiota may support the olive plant to ward off detrimental pathogens. It further yields five promising candidates for the development of eco‐friendly, multi‐active biocontrol agents in olive agriculture. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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“…(both members of the Bacillus cereus clade). This clearly exemplifies the challenges associated with the identification of members of the Bacillaceae family at species level 39,40 …”

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Antimicrobial profile of the culturable olive sporobiota and its potential as a source of biocontrol agents for major phytopathogens in olive agriculture

Manetsberger,

Caballero Gómez,

Benomar

et al. 2023

Pest Management Science

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Quality Control in Foods, State of the Art and Prospects

Reyes

García

2016

Encyclopedia of Analytical Chemistry

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Quality control (QC) of food includes control of authenticity, origin, properties or attributes, composition, and safety involving all steps of the global supply chain – from the raw material up to the final product sold on the supermarket shelf. Despite the great concern regarding their quality, these products are currently monitored and certified by means of paper documentation, some targeted analysis, and sensory properties. This article encompasses a revision on the instrumental analytical techniques, methods, and systems used in food QC together with their main applications and a brief historical perspective on food analysis, together with a deep revision on the current state of art of modern analytical instruments, methodologies, and applications in the QC of food in order to establish Quo Vadis QC of foods. This article also discusses the present and future challenges in QC of food, the need for faster techniques, the prospect of advanced imaging techniques, and the application of ‘omic’ in food QC (including metabolomics, proteomics, etc.)

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Development of a Taxon-Specific Real-Time PCR Method Targeting the Bacillus subtilis Group to Strengthen the Control of Genetically Modified Bacteria in Fermentation Products

et al. 2022

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Most of the bacteria that are used to produce fermentation products, such as enzymes, additives and flavorings, belong to the Bacillus subtilis group. Recently, unexpected contaminations with unauthorized genetically modified (GM) bacteria (viable cells and associated DNA) that were carrying antimicrobial resistance (AMR) genes was noticed in several microbial fermentation products that have been commercialized on the food and feed market. These contaminations consisted of GM Bacillus species belonging to the B. subtilis group. In order to screen for the potential presence of such contaminations, in this study we have developed a new real-time PCR method targeting the B. subtilis group, including B. subtilis, B. licheniformis, B. amyloliquefaciens and B. velezensis. The method’s performance was successfully assessed as specific and sensitive, complying with the Minimum Performance Requirements for Analytical Methods of GMO Testing that is used as a standard by the GMO enforcement laboratories. The method’s applicability was also tested on 25 commercial microbial fermentation products. In addition, this method was developed to be compatible with the PCR-based strategy that was recently developed for the detection of unauthorized GM bacteria. This taxon-specific method allows the strengthening of the set of screening markers that are targeting key sequences that are frequently found in GM bacteria (AMR genes and shuttle vector), reinforcing control over the food and feed chain in order to guarantee its safety and traceability.

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Development of a Novel PCR Primer to Differentiate and IdentifyBacillus subtilisand Closely Related Species Isolated from Thai Fermented Foods

Cited by 3 publications

References 20 publications

Antimicrobial profile of the culturable olive sporobiota and its potential as a source of biocontrol agents for major phytopathogens in olive agriculture

Antimicrobial profile of the culturable olive sporobiota and its potential as a source of biocontrol agents for major phytopathogens in olive agriculture

Quality Control in Foods, State of the Art and Prospects

Development of a Taxon-Specific Real-Time PCR Method Targeting the Bacillus subtilis Group to Strengthen the Control of Genetically Modified Bacteria in Fermentation Products

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