Development of a Novel Robust Artificial Intelligence Developed Sperm Dna Fragmentation Test – Preliminary Findings

Kuroda, Shinnosuke; Karna, Keshab Kumar; Kaiyal, Raneen Sawaid; Gupta, Sajal; Sharma, R.; Agarwal, Ashok

doi:10.1016/j.fertnstert.2022.09.064

Cited by 5 publications

(4 citation statements)

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“…However, we still have some way to travel. The development of novel consensus methods for detecting DNA damage, 14 determining which patients should be assessed, 30 elucidation of the relative importance of single and double strand breaks, 31 the significance of oxidative stress in the induction of such damage, 26 the potential therapeutic role for antioxidants, 32 and the consequences of sperm DNA damage in terms of male fertility and the health and wellbeing of the offspring, 23,24,26 all need to be elucidated before the true clinical significance of such damage becomes clear.…”

Section: Discussionmentioning

confidence: 99%

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Sperm DNA integrity: a special issue exploring the causes, consequences, and treatment of DNA damage in human spermatozoa

Aitken

2023

Andrology

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Since the pioneering studies by Don Evenson and colleagues first opened our eyes to the existence of DNA damage in subpopulations of human spermatozoa, 1 important questions have been raised concerning the causes, clinical significance, and resolution of this phenomenon. 2 In this Special Issue of Andrology, we have brought together a collection of cutting-edge research articles and reviews, reflecting the most recent advances in this area. We hope that this collection of articles will help andrologists recognize the importance of DNA damage in spermatozoa, understand where the knowledge gaps are, and thoughtfully engage in research designed to enhance our capacity to diagnose and manage this critical element of semen quality. OXIDATIVE STRESS AND SPERM DNA DAMAGEIn terms of the etiology of DNA damage, there appears to be a general consensus that oxidative stress makes a major contribution to its induction. 2 As highlighted in this Special Issue, we are beginning to gather a detailed understanding as to why oxidative stress is so important in the causation of sperm DNA damage, as well as the key role that enzymes such as topoisomerase might play in this process. 2 However, the source of the oxidative stress driving this DNA damage has remained largely unresolved. A complication in this regard is that every semen sample is contaminated with leukocytes, particularly neutrophils, and these cells are many times more active in generat-

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Section: Discussionmentioning

confidence: 99%

“…and others 10–13 . Moreover, Kuroda et al 14 . observed no significant correlation between sperm DNA damage and oxidative stress as measured by the MiOXSYS ® system, despite the abundance of data revealing the fundamental importance of free radical attack in the induction of sperm DNA fragmentation 15 .…”

Section: Oxidative Stress and Sperm Dna Damagementioning

confidence: 98%

Sperm DNA integrity: a special issue exploring the causes, consequences, and treatment of DNA damage in human spermatozoa

Aitken

2023

Andrology

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“…Addressing the variations in cutoff values and subjectivity in SDF assays is crucial for its broader application. Artificial intelligence (AI) could play a significant role in this regard, as recent studies combining SDF techniques with AI have shown promising results 137,138 . Such attempts may reduce human effort and minimize the subjectivity and inter‐variability in assays, which have been obstacles in determining the efficacy of SDF.…”

Section: Discussionmentioning

confidence: 99%

Sperm DNA fragmentation testing in clinical management of reproductive medicine

Adler,

Roth,

Lundy

et al. 2023

Reprod Medicine & Biology

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BackgroundApproximately 8%–12% of couples worldwide face infertility, with infertility of individuals assigned male at birth (AMAB) contributing to at least 50% of cases. Conventional semen analysis commonly used to detect sperm abnormalities is insufficient, as 30% of AMAB patients experiencing infertility show normal results in this test. From a genetic perspective, the assessment of sperm DNA fragmentation (SDF) is important as a parameter of sperm quality.MethodsIn this narrative study, we review and discuss pathophysiological causes, DNA repair mechanisms, and management of high SDF. We then summarize literature exploring the association between SDF and reproductive outcomes.Main FindingsRecent systematic reviews and meta‐analyses have revealed a significant association between high SDF in AMAB individuals and adverse reproductive outcomes including embryo development, natural conception, intrauterine insemination, and in vitro fertilization. However, the association with live birth rates and pregnancy rates following intracytoplasmic injection remains inconclusive. The disparities among quantitative assays, inconsistent reference range values, absent high‐quality prospective clinical trials, and clinical heterogeneity in AMAB patients with elevated SDF represent the main limitations affecting SDF testing.ConclusionThe evaluation and management of SDF plays an important role in a subset of AMAB infertility, but widespread integration into clinical guidelines will require future high‐quality clinical trials and assay standardization.

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“…McCallum et al described a CNN trained using a set of 1064 images of individual sperm cells of known DNA integrity to provide a DNA integrity prediction from a single bright-field image in under 10 ms 62 . Recently, Kuroda et al described further progress with their AI-augmented sperm chromatin dispersion (SCD) test kit capable of assessing DNA fragmentation in >5000 spermatozoa at once, compared to a limited 300 in the widely commercially-used Halosperm SCD test 63 . The improved kit showed a good correlation with the conventional test that requires manual counting (Halosperm G2; r = 0.69, p = 0.02).…”

Section: Dna Fragmentationmentioning

confidence: 99%

The prospect of artificial intelligence to personalize assisted reproductive technology

Hanassab,

Abbara,

Yeung

et al. 2024

npj Digit. Med.

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Infertility affects 1-in-6 couples, with repeated intensive cycles of assisted reproductive technology (ART) required by many to achieve a desired live birth. In ART, typically, clinicians and laboratory staff consider patient characteristics, previous treatment responses, and ongoing monitoring to determine treatment decisions. However, the reproducibility, weighting, and interpretation of these characteristics are contentious, and highly operator-dependent, resulting in considerable reliance on clinical experience. Artificial intelligence (AI) is ideally suited to handle, process, and analyze large, dynamic, temporal datasets with multiple intermediary outcomes that are generated during an ART cycle. Here, we review how AI has demonstrated potential for optimization and personalization of key steps in a reproducible manner, including: drug selection and dosing, cycle monitoring, induction of oocyte maturation, and selection of the most competent gametes and embryos, to improve the overall efficacy and safety of ART.

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Development of a Novel Robust Artificial Intelligence Developed Sperm Dna Fragmentation Test – Preliminary Findings

Cited by 5 publications

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Sperm DNA integrity: a special issue exploring the causes, consequences, and treatment of DNA damage in human spermatozoa

Sperm DNA integrity: a special issue exploring the causes, consequences, and treatment of DNA damage in human spermatozoa

Sperm DNA fragmentation testing in clinical management of reproductive medicine

The prospect of artificial intelligence to personalize assisted reproductive technology

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