Development of a novel validated method for aflatoxin analysis on edible bird nest

Abstract

Edible bird nest (EBN) is an excellent export commodity from Indonesia and South East Asia countries. To keep the quality assurance of EBN, aflatoxin analysis should be conducted to check its biological hazard. This research was conducted to develop a rapid, sensitive and validated method to detect aflatoxin contamination in EBN. This study showed that in-house AF.2 IAC containing AF.2 monoclonal antibody can be used for EBN sample clean-up. Validation methods were measured using parameters which were recovery (86.5-96.3% for AFB1, 87.8-98.5% for AFB2, 82.8-100.5 for AFG1, 79.8-94.3% for AFG2), intra-day repeatability RSD (⩽ 6.4% for all aflatoxins), linearity (⩾ 0.99997 for all aflatoxins), limit of detection (0.26 ng/g for AFB1, 0.04 ng/g AFB2, 0.26 ng/g for AFG1, 0.03 ng/g for AFG2) and limit of quantification (0.79 ng/g for AFB1, 0.13 ng/g AFB2, 0.81 ng/g for AFG1, 0.09 ng/g for AFG2). These results showed that both recovery test of spiked PBS and recovery test of 3-level aflatoxin concentration spiked IBN were good, thus in-house AF.2 IAC can be used to analyze aflatoxins in EBN. Based on validation parameters such as recovery test, intra-day repeatability, linearity, LOD, and LOQ which had been tested, our method can be used for validated method for aflatoxin analysis in EBN. Further analysis of aflatoxins in edible bird nest will be reported separately.