Development of a PCR-based mating-type assay for Clavicipitaceae

Yokoyama, Eiji; Yamagishi, Kenzo; Hara, Akira

doi:10.1111/j.1574-6968.2004.tb09697.x

Cited by 15 publications

(54 citation statements)

References 28 publications

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“…PCR products were purified using a Qiagen QIAquick Gel Extraction Kit and then sent for direct sequencing in both directions using the same primer pair. The 500 bp long ITS sequence obtained (GenBank accession number: DQ374646) was identical with that of P. farinosus strain IFO7866 reported by Yokoyama et al (2004) (GenBank accession number AB083033) and also with some other ITS sequences of various P. farinosus strains available in DNA databases, thus confirming the identity of our isolate MYA-3388.…”

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confidence: 80%

Paecilomyces farinosus destroys powdery mildew colonies in detached leaf cultures but not on whole plants

et al. 2006

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Since 2001, several isolates of Blumeria graminis, the causal agent of cereal powdery mildew, maintained on detached leaves at the John Innes Centre, Norwich, UK, have spontaneously become infected with an unknown filamentous fungus whose mycelia have quickly overgrown the powdery mildew colonies and destroyed them completely. A total of five isolates of the contaminant were obtained and identified as Paecilomyces farinosus based on morphological characteristics and rDNA ITS sequence data. To determine whether these P. farinosus isolates can be considered as biocontrol agents (BCAs) of powdery mildews, we studied the interactions between P. farinosus and the following four powdery mildew species: B. graminis f.sp. hordei infecting barley, Oidium neolycopersici infecting tomato, Golovinomyces orontii infecting tobacco and Podosphaera fusca infecting cucumber. The powdery mildew colonies of all these four powdery mildew species were quickly destroyed by P. farinosus in leaf cultures but neither conidial suspensions nor cell-free culture filtrates of P. farinosus isolates could suppress the spread of powdery mildew infections on diseased barley, tomato, tobacco or cucumber plants in the greenhouse. It is concluded that P. farinosus cannot be considered as a promising BCA of powdery mildew infections although it can destroy powdery mildew colonies in detached leaf cultures and can be a menace during the maintenance of such cultures of cereal, apple, cucurbit and tomato powdery mildew isolates.Powdery mildew fungi are obligate biotrophic parasites of many plants and as such can survive in living host plant tissues only. For various research purposes, many laboratories maintain isolates of different powdery mildew fungi on detached leaves or leaf segments of cereals (Brown and Wolfe

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confidence: 80%

Paecilomyces farinosus destroys powdery mildew colonies in detached leaf cultures but not on whole plants

et al. 2006

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“…In contrast, DNA-based approaches are relatively inexpensive and usually provide reliable mating type assignments (Cherif et al 2006;Dyer et al 2001;Yokoyama et al 2004). These DNA-based methods are, however, dependent on the availability of sequence information for the MAT locus, because MAT idiomorph-specific PCR assays exploit the inherent differences in the MAT genes (Dyer et al 2001;Steenkamp et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes

et al. 2012

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In heterothallic Ascomycota, two opposite but distinct mating types control all sexual processes. Using mating crosses on agar plates, the heterothallic nature of the wood-inhabiting fungus Ophiostoma quercus was confirmed and mating types were assigned to 10 isolates. Primers were subsequently designed to target both the mating type 1 (MAT1-1) and 2 (MAT1-2) idiomorphs in these isolates. The results showed that all isolates contained sequence fragments representing both idiomorphs. This was unexpected, as each isolate from a heterothallic species would typically contain only one of the two possible MAT idiomorphs. An atypical mating system such as the one described in this study has not previously been encountered in any other Ascomycota mating locus described to date.

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“…The 18S rDNA nucleotide sequence of the isolate 8-2 was identical to that of P. nostocoides JCM 8437, accession number AB104884, in the GenBank database (Yokoyama et al 2004). Therefore, we named this isolate P. nostocoides NTU-FC-LP01 and deposited it in the Bioresource Collection and Research Center (BCRC) in Taiwan, with the accession number BCRC 34144.…”

Section: Screening Testsmentioning

confidence: 99%

A strategy for screening microbial strains with lipolytic specificity toward monoacylglycerols

Huang

Wang

et al. 2009

World J Microbiol Biotechnol

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For easy obtaining the microorganisms with lipolytic specificity toward monoacylglycerols, we developed a simple and effective method to isolate the objective strains. This method employed a nile-blue agar-plate culture containing mono-and tri-acylglycerols for microorganism screening and selected the desired microorganisms by analysis of free fatty acid contents in lipid extracts obtained from culture broth. Using this strategy, we successfully isolated one mold strain with superior lipolytic ability for the hydrolysis of monoacylglycerols. The mold was identified and designated as Paecilomyces nostocoides NTU-FC-LP01. The lyophilized mycelia of the isolated mold used as a biocatalyst showed high specificity toward monoacylglycerols rather than di-and tri-acylglycerols. Furthermore, the lyophilized mycelia catalyzed the monoolein synthesis through the direct esterification of oleic acid and glycerol. It indicated that the lyophilized mycelia of the present P. nostocoides NTU-FC-LP01 could be used as a natural immobilized biocatalyst for the glycerol/oleic acid esterification to produce monoolein.

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Development of a PCR-based mating-type assay for Clavicipitaceae

Cited by 15 publications

References 28 publications

Paecilomyces farinosus destroys powdery mildew colonies in detached leaf cultures but not on whole plants

Paecilomyces farinosus destroys powdery mildew colonies in detached leaf cultures but not on whole plants

Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes

A strategy for screening microbial strains with lipolytic specificity toward monoacylglycerols

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