Development of a PCR Lateral Flow Assay for Rapid Detection of Bacillus anthracis, the Causative Agent of Anthrax

Banger, Swati; Pal, Vijai; Tripathi, Nagesh K.; Goel, Ajay

doi:10.1007/s12033-021-00335-6

Cited by 5 publications

(3 citation statements)

References 25 publications

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“…Bacillus anthracis is the causative agent of anthrax in ungulates and humans . It is a thermotolerant pathogen occasionally associated with food poisoning 36 . Furthermore, the level of sanitary risk appeared to be dependent on the assignment of the strain present.…”

Section: Discussionmentioning

confidence: 99%

Midguts of Culex pipiens L. (Diptera: Culicidae) as a potential source of raw milk contamination with pathogens

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Hegazy

Kamal

et al. 2022

Sci Rep

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Despite their importance, only few studies focused on the mosquitoes borne microbial diseases, especially bacterial and fungal diseases, their vectorial capacity toward microorganisms, and their important role in raw milk contamination with pathogens in some unsanitary dairy farms. In Egypt, where Culex pipiens is the historical main vector of lymphatic filariasis, only few studies discussed the isolation of pathogens from the midguts of different stages of C. pipiens. This study aims to isolate and identify the pathogenic symbiotic microorganisms inside the midgut of adult female C. pipiens as well as investigate its ability to transmit their midgut pathogens to raw milk. A total of 750 field strain C. pipiens larvae of the second and third larval instars were collected from ponds water around the livestock farms in Mariotteya, Giza, Egypt, for microbial pathogen isolation and identification. All collected larval instars were transported to the laboratory at the Research and Training Center on Vectors of Diseases (RTC), Ain Shams University, where they were maintained for further studies. Six groups of C. pipiens were tested for the incidence of various pathogenic microorganisms in their midguts and their possibility to contaminate commercial sterilized milk. Traditional PCR assays and sequencing method detected and identified 16srRNA genes of the predominant hemolytic isolates from milk and midguts of female C. pipiens. The phylogenetic analyses of the obtained isolates were performed based on NCBI data. Three strains of Bacillus anthracis strain CPMESA 2021, Staphylococcus warneri strain CPSAME 2021, and Bacillus cereus strain CPSEMA 2021, which represent most food pathogens, were found in the midguts of C. pipiens and were submitted to the GenBank database with the accession numbers OK585071, OK576651, and OK585052, respectively. The isolation of these strains from mosquitoes raises contemporary issues concerning milk safety, such as bacterial isolates, the degree of the vectorial capacity of mosquitoes, milk production and processing conditions, and human pathogenicity. Such serious issues need further investigation.

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Section: Discussionmentioning

confidence: 99%

Midguts of Culex pipiens L. (Diptera: Culicidae) as a potential source of raw milk contamination with pathogens

Adly

Hegazy

Kamal

et al. 2022

Sci Rep

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“…The diversity of such threatening agents and their varying degrees of infectivity and transmissibility rendered their detection highly challenging. [ 201 ] Established detection methods such as genome sequencing, [ 202 ] enzyme‐linked immunosorbent assay (ELISA), [ 203 ] and polymerase chain reaction (PCR) [ 204 ] are being continuously developed to overcome these challenges. However, these methods are typically used only after a diagnosis of the pathogen is suspected by a clinician.…”

Section: Surface Biofunctionalized Pnps: Biomedical Applicationsmentioning

confidence: 99%

Surface Bio‐engineered Polymeric Nanoparticles

Haidar,

Bilek,

Akhavan

2024

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Surface bio‐engineering of polymeric nanoparticles (PNPs) has emerged as a cornerstone in contemporary biomedical research, presenting a transformative avenue that can revolutionize diagnostics, therapies, and drug delivery systems. The approach involves integrating bioactive elements on the surfaces of PNPs, aiming to provide them with functionalities to enable precise, targeted, and favorable interactions with biological components within cellular environments. However, the full potential of surface bio‐engineered PNPs in biomedicine is hampered by obstacles, including precise control over surface modifications, stability in biological environments, and lasting targeted interactions with cells or tissues. Concerns like scalability, reproducibility, and long‐term safety also impede translation to clinical practice. In this review, these challenges in the context of recent breakthroughs in developing surface‐biofunctionalized PNPs for various applications, from biosensing and bioimaging to targeted delivery of therapeutics are discussed. Particular attention is given to bonding mechanisms that underlie the attachment of bioactive moieties to PNP surfaces. The stability and efficacy of surface‐bioengineered PNPs are critically reviewed in disease detection, diagnostics, and treatment, both in vitro and in vivo settings. Insights into existing challenges and limitations impeding progress are provided, and a forward‐looking discussion on the field's future is presented. The paper concludes with recommendations to accelerate the clinical translation of surface bio‐engineered PNPs.

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“…The usage of colloidal gold nanoparticles in immunoassays gained considerable attention in the 1980s because the well-known pregnancy test strip was commercially available and patented [18]. Eventually, the LFA was developed for determining antigens, antibodies, and amplification products of genes [19][20][21][22] in several fields. This method is suitable as a point-of-care test, according to the World Health Organization using the criteria of ASSURED (affordable, sensitive, specific, userfriendly, robust, equipment-free, and deliverable) [23].…”

Section: Lfamentioning

confidence: 99%

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

et al. 2022

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Phytoproducts are involved in various fields of industry. Small-molecule (Mw < 900 Da) organic compounds can be used to indicate the quality of plant samples in the perspective of efficacy by measuring the necessary secondary metabolites and in the perspective of safety by measuring the adulterant level of toxic compounds. The development of reliable detection methods for these compounds in such a complicated matrix is challenging. The lateral flow immunoassay (LFA) is one of the immunoassays well-known for its simplicity, portability, and rapidity. In this review, the general principle, components, format, and application of the LFA for phytoproducts are discussed.

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Development of a PCR Lateral Flow Assay for Rapid Detection of Bacillus anthracis, the Causative Agent of Anthrax

Cited by 5 publications

References 25 publications

Midguts of Culex pipiens L. (Diptera: Culicidae) as a potential source of raw milk contamination with pathogens

Midguts of Culex pipiens L. (Diptera: Culicidae) as a potential source of raw milk contamination with pathogens

Surface Bio‐engineered Polymeric Nanoparticles

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

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