Development of a PCR procedure for the detection of a herpes-like virus infecting oysters in France

Renault, Tristan; Deuff, R M Le; Lipart, Cécile; Delsert, Claude

doi:10.1016/s0166-0934(00)00175-0

Cited by 77 publications

(99 citation statements)

References 22 publications

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“…Since that time, larval Pacific oyster losses have recurred annually in France and adjacent countries during the summer months (Renault et al 1994a(Renault et al ,b, 2000b. The same or similar herpes-like virus(es) has been reported in other oyster species including flat oysters Ostrea angasi and Ostrea chilensis in Australia and New Zealand (Hine & Thorne 1997, Hine et al 1998 and Ostrea edulis in France (Renault et al 2000b). Other ABSTRACT: Pacific Crassostrea gigas and eastern C. virginica oysters were examined between June 2002 and April 2003 from 8 locations along the east, west and south USA coasts for oyster herpes virus (OsHV) infections using the A primer set in a previously developed PCR test.…”

Section: Introductionsupporting

confidence: 59%

“…Interestingly, the OsHV found in Tomales Bay oysters was amplified by the nested A primers but not by primers C2/C6, unlike the OsHV-1 characterized from France which amplified with both primer sets (Renault et al 2000b). Arzul et al (2001) described a variant of the oyster herpes virus, which they found in Crassostrea gigas and Venerupis philippinarum samples from one French hatchery in Normandy.…”

Section: Discussionmentioning

confidence: 92%

“…Recent observations question the role of phytoplankton blooms in these losses (Righetti 2002 Table 3. PCR examination of 89 Tomales Bay Pacific oysters using the standard volume (1 µl) employed by Renault et al (2000b) and 500 ng of nucleic acid template, or both amounts of template. na: not applicable OsHV prevalences in surviving Tomales Bay oysters roughly corresponded to the proportion of dead oysters within our samples.…”

Section: Histologymentioning

confidence: 99%

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Herpes virus in juvenile Pacific oysters Crassostrea gigas from Tomales Bay, California, coincides with summer mortality episodes

Friedman¹,

Estes²,

Stokes³

et al. 2005

Dis. Aquat. Org.

165

132

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Section: Introductionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 92%

Section: Histologymentioning

confidence: 99%

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Herpes virus in juvenile Pacific oysters Crassostrea gigas from Tomales Bay, California, coincides with summer mortality episodes

Friedman¹,

Estes²,

Stokes³

et al. 2005

Dis. Aquat. Org.

165

132

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“…data). Herpes-like virus purification from infected C. gigas larvae and virus DNA extraction (Le Deuff & Renault 1999) has permitted the development of specific molecular tools (Renault & Lipart 1998, Renault et al 2000b, and molecular analysis suggests that the same virus infects several bivalve species belonging to different genera. Thus, the detection of a herpes-like virus in a particular bivalve batch presenting anomalous mortalities may indicate that precautions should be taken in order to avoid the spread of the viral infection to all breedings.…”

Section: Discussionmentioning

confidence: 99%

A herpes-like virus infects a non-ostreid bivalve species: virus replication in Ruditapes philippinarum larvae

Renault

Lipart²,

Arzul³

2001

Dis. Aquat. Org.

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Sporadic high mortalities were reported in June 1997 among hatchery-reared larval Manila clam Ruditapes philippinarum in a French commercial hatchery. Cellular abnormalities were observed in semi-thin sections in affected animals. Transmission electron microscopy revealed the presence of herpes-like virus particles in larvae. This is the first description of a herpes-like virus infection in larval R. philippinarum, a non-ostreid bivalve species. Virus particles were similar to other herpes-like viruses described from different oyster species with respect to ultrastructure and morphogenesis. Electron microscopic examination also demonstrated cells with condensed chromatin and extensive perinuclear fragmentation of chromatin. Like viruses infecting oysters, the herpes-like virus detected in clams may induce apoptosis in infected animals. KEY WORDS: Herpes-like virus · Manila clam · Ruditapes philippinarum · Mortality · Virus replication · Apoptosis Resale or republication not permitted without written consent of the publisherDis Aquat Org 45: [1][2][3][4][5][6][7] 2001 scopy. The larvae were fixed in 2.5% glutaraldehyde in 0.2 M cacodylate buffer at pH 7.2 for 1 h at 4°C. After 2 washes in cacodylate buffer, samples were post-fixed in 1% osmium tetroxide in the same buffer at 4°C. Larval samples were dehydrated by serial ethanol baths (70 to 100%), partially rehyrated by two 10 min baths in 0.2 M cacodylate buffer and de-calcified with a 2% ethylenetetra-acetic acid (EDTA) solution in 0.2 M cacodylate buffer overnight at 4°C. After fixation and post-fixation, larvae were washed in 0.2 M cacodylate buffer, dehydrated by means of an ascending ethanol series (70 to 100%), cleared twice for 15 min in propylene oxide and infiltrated for 1 h in 50:50 propylene oxide:Epon resin. After 1 h infiltration in pure Epon resin, they were embedded in resin and cured for 48 h at 60°C. Sections were cut at 1 µm for light microscopic examination which were stained in 0.5% toluidine blue in 1% sodium borate solution. Ultrathin sections were stained in 5% uranyl acetate in 50% ethanol for 20 min and in 5% lead citrate in fresh boiled distilled water for 3 min. They were examined in a JEOL JEM 1200 EX transmission electron microscope at 80 kV. RESULTS Course of the diseaseA few days after spawning, Ruditapes philippinarum larvae showed reduction in feeding and swimming activities. Mortality occurred, at the beginning of June 1997, with total mortality by Day 13. Affected larvae swam weakly in circles before dying appromimately at 100 to 150 µm in size. Histological examinationAbnormal nuclei were present throughout the connective tissue in semi-thin sections of moribund larvae. Enlarged nuclei with abnormal marginated chromatin were evident in fibroblastic-like cells (Fig. 1). Highly condensed nuclei were observed in ovoid cells interpreted as haemocytes. Some nuclei of fibroblastic-like cells and haemocytes appeared with a ring-shaped or crescent-shaped chromatin (Fig. 1). The lesions were mainly confined to connective t...

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“…Par la suite, des virus de type herpès ont été mis en évidence dans un large spectre de bivalves dont différentes espèces d'huîtres, de palourdes et la coquille Saint Jacques (Renault & Novoa, 2004). Ils ont ainsi été décrits aux USA (Farley et al 1972, Friedman et al 2005, Meyers et al 2009), en Nouvelle Zélande (Hine et al 1992 ;Hine et al 1998), en France (Nicolas et al 1992 ;Comps & Cochennec, 1993 ;Renault et al 1994aRenault et al , 1994bRenault et al 2000aRenault et al , 2000bArzul et al 2001aArzul et al , 2001bArzul et al , 2001cRenault et al 2001aRenault et al , 2001b, en Australie (Hine & Thorne, 1997) (Hooper et al 2007 ;Tan et al 2008) associés à des épi-sodes de mortalité massive à Taiwan et en Australie. Un variant du virus, dénommé OsHV-1var, a été rapporté chez différentes espèces de bivalves incluant l'huître creuse C. gigas (Arzul et al 2001a ;2001b, 2001c.…”

Section: Virus De Type Herpès Et Herpèsvirus Infectant Les Mollusquesunclassified

Les virus infectant les mollusques marins: un exemple d'actualité, les herpèsvirus

Renault¹

2011

bavf

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Bien que des épisodes de mortalité associés à la détection de virus aient été rapportés chez différentes espèces de mollusques, peu d'informations sont aujourd'hui disponibles concernant l'affiliation exacte de ces virus et leur position taxinomique. Parmi les virus infectant les coquillages, les herpèsvirus ont fait l'objet d'une attention particulière du fait de leurs impacts économiques et éco-logiques sur les animaux en élevage et sauvages. Des recherches par des approches de biologie moléculaire ont été menées en France sur le virus infectant les huîtres creuses, Crassostrea gigas. Sur la base des données moléculaires et ultra-structurales, ce virus a été classé, sous le nom d'ostreid herpesvirus 1 (OsHV-1), comme première espèce virale de la famille Malacoherpesviridae. Depuis 2008, des épisodes de surmortalité ont été rapportés dans différentes régions de production des huîtres creuses C. gigas, en France, en Irlande et au Royaume Uni. Ils ont été attribués à des conditions environnementales défavorables associées à la découverte d'un variant génotypique viral particulier, appelé OsHV-1 µVar.

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Development of a PCR procedure for the detection of a herpes-like virus infecting oysters in France

Cited by 77 publications

References 22 publications

Herpes virus in juvenile Pacific oysters Crassostrea gigas from Tomales Bay, California, coincides with summer mortality episodes

Herpes virus in juvenile Pacific oysters Crassostrea gigas from Tomales Bay, California, coincides with summer mortality episodes

A herpes-like virus infects a non-ostreid bivalve species: virus replication in Ruditapes philippinarum larvae

Les virus infectant les mollusques marins: un exemple d'actualité, les herpèsvirus

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