Development of a Peptide Derived from Platelet-Derived Growth Factor (PDGF-BB) into a Potential Drug Candidate for the Treatment of Wounds

Deptuła, Milena; Karpowicz, Przemysław; Wardowska, Anna; Sass, Piotr; Sosnowski, Paweł; Mieczkowska, Alina; Filipowicz, Natalia; Dzierżyńska, Maria; Sawicka, Justyna; Nowicka, Ewa; Langa, Paulina; Schumacher, Adriana; Cichorek, M; Zieliński, Jacek; Kondej, Karolina; Kasprzykowski, Franciszek; Czupryn, Artur; Janus, Łukasz; Mucha, Piotr; Skowron, Piotr; Piotrowski, Arkadiusz; Sachadyn, Paweł; Rodziewicz‐Motowidło, Sylwia; Pikuła, Michał

doi:10.1089/wound.2019.1051

Cited by 34 publications

(47 citation statements)

References 71 publications

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“…In our previous work [ 26 ], we optimized a procedure to use fibroblasts, keratinocytes, and ASCs as models in skin regeneration studies. We used serum free culture media, because addition of FBS, which contains various growth factors, which stimulate cell proliferation or migration, could mask IM’s effect.…”

Section: Discussionmentioning

confidence: 99%

“…We also assessed the allergic potential of IM peptide, using an in vitro method, namely the BAT described previously [ 26 ]. The conducted tests showed low allergic potential of IM peptide, thus confirming the immunological safety of this peptide.…”

Section: Discussionmentioning

confidence: 99%

“…High-throughput next generation sequencing of RNA which can reveal mechanisms of action of tested pharmacological agents, thanks to its potential to characterize and quantify genome-wide gene expression [ 43 ]. Utilization of this technology for investigating the effect of PDGF2 peptide administration on skin cells is presented in our previous research [ 26 ]. In this work, we also performed RNA sequencing analysis for ASCs stimulated with IM.…”

Section: Discussionmentioning

confidence: 99%

“…Cell death was normalized with respect to untreated control (without IM). Non-IM treated cells were used as negative control (0%) and Triton-X 100 (1%) was used as the positive control for maximum LDH release (maximum cytotoxicity, 100%) [ 26 , 62 ]. The assay was performed to IM in concentrations of 50–150 µg/mL.…”

Section: Methodsmentioning

confidence: 99%

“…Plates were then read using a standard plate reader at OD 490 nm. Cell proliferation was normalized with respect to an untreated control (100%) [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

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Imunofan—RDKVYR Peptide—Stimulates Skin Cell Proliferation and Promotes Tissue Repair

et al. 2020

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Regeneration and wound healing are vital to tissue homeostasis and organism survival. One of the biggest challenges of today’s science and medicine is finding methods and factors to stimulate these processes in the human body. Effective solutions to promote regenerative responses will accelerate advances in tissue engineering, regenerative medicine, transplantology, and a number of other clinical specialties. In this study, we assessed the potential efficacy of a synthetic hexapeptide, RDKVYR, for the stimulation of tissue repair and wound healing. The hexapeptide is marketed under the name “Imunofan” (IM) as an immunostimulant. IM displayed stability in aqueous solutions, while in plasma it was rapidly bound by albumins. Structural analyses demonstrated the conformational flexibility of the peptide. Tests in human fibroblast and keratinocyte cell lines showed that IM exerted a statistically significant (p < 0.05) pro-proliferative activity (30–40% and 20–50% increase in proliferation of fibroblast and keratinocytes, respectively), revealed no cytotoxicity over a vast range of concentrations (p < 0.05), and had no allergic properties. IM was found to induce significant transcriptional responses, such as enhanced activity of genes involved in active DNA demethylation (p < 0.05) in fibroblasts and activation of genes involved in immune responses, migration, and chemotaxis in adipose-derived stem cells derived from surgery donors. Experiments in a model of ear pinna injury in mice indicated that IM moderately promoted tissue repair (8% in BALB/c and 36% in C57BL/6 in comparison to control).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Plates were then read using a standard plate reader at OD 490 nm. Cell proliferation was normalized with respect to an untreated control (100%) [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

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Imunofan—RDKVYR Peptide—Stimulates Skin Cell Proliferation and Promotes Tissue Repair

et al. 2020

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Functions of immunologically active peptides and their roles in intestinal inflammation

Wang,

Li,

Huang

et al. 2024

Food Frontiers

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Immunologically active peptides are a class of peptides with immune functions such as enhancing body immunity, regulating cell growth and differentiation, migration, chemotaxis, neurotransmission, and inducing apoptosis of tumor cells. They have many features, such as small molecular weight, strong stability, high biological activity, easy to adjust the structure accurately, and easy to synthesize. Exploring the mechanism of action of immunologically active peptides can help the modification and development of immunologically active peptides. In this article, the functional properties of immunologically active peptides are described from the perspectives of structural classification of immunologically active peptides and regulatory mechanisms. The relationship between structure and function and the application of different mechanisms in diseases are reviewed, focusing on the role in intestinal inflammation.

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Adipose‐derived stromal cells for nonhealing wounds: Emerging opportunities and challenges

Deptuła

Brzezicka

Skoniecka

et al. 2021

Medicinal Research Reviews

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Wound healing complications affect thousands of people each year, thus constituting a profound economic and medical burden. Chronic wounds are a highly complex problem that usually affects elderly patients as well as patients with comorbidities such as diabetes, cancer (surgery, radiotherapy/chemotherapy) or autoimmune diseases. Currently available methods of their treatment are not fully effective, so new solutions are constantly being sought. Cell‐based therapies seem to have great potential for use in stimulating wound healing. In recent years, much effort has been focused on characterizing of adipose‐derived mesenchymal stromal cells (AD‐MSCs) and evaluating their clinical use in regenerative medicine and other medical fields. These cells are easily obtained in large amounts from adipose tissue and show a high proregenerative potential, mainly through paracrine activities. In this review, the process of healing acute and nonhealing (chronic) wounds is detailed, with a special attention paid to the wounds of patients with diabetes and cancer. In addition, the methods and technical aspects of AD‐MSCs isolation, culture and transplantation in chronic wounds are described, and the characteristics, genetic stability and role of AD‐MSCs in wound healing are also summarized. The biological properties of AD‐MSCs isolated from subcutaneous and visceral adipose tissue are compared. Additionally, methods to increase their therapeutic potential as well as factors that may affect their biological functions are summarized. Finally, their therapeutic potential in the treatment of diabetic and oncological wounds is also discussed.

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Development of a Peptide Derived from Platelet-Derived Growth Factor (PDGF-BB) into a Potential Drug Candidate for the Treatment of Wounds

Cited by 34 publications

References 71 publications

Imunofan—RDKVYR Peptide—Stimulates Skin Cell Proliferation and Promotes Tissue Repair

Imunofan—RDKVYR Peptide—Stimulates Skin Cell Proliferation and Promotes Tissue Repair

Functions of immunologically active peptides and their roles in intestinal inflammation

Adipose‐derived stromal cells for nonhealing wounds: Emerging opportunities and challenges

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