Development of a Platelet Lysate–Based Printable, Transparent Biomaterial With Regenerative Potential for Epithelial Corneal Injuries

Frazer, Hannah; You, Jungmok; Chen, Zhi; Sayyar, Sepidar; Liu, Xiao; Taylor, Adam; Hodge, Chris; Wallace, Gordon G.; Sutton, Gerard

doi:10.1167/tvst.9.13.40

Cited by 7 publications

(9 citation statements)

References 29 publications

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“…7 , 8 Our hPL based bio-glue contains much less of fibrinogen at 2 mg/mL, which mirrors the physiological concentration in blood. 5 Our earlier work confirmed its transparency, adhesion to corneal tissue, and ability to accelerate corneal epithelial cell wound healing. Further, its shear thinning properties make it compatible with an extrusion-based printing processes.…”

Section: Introductionsupporting

confidence: 62%

“…Our group previously shown by combining human platelet lysate (hPL) with low concentrations of fibrinogen and thrombin, a transparent fibrin-based gel can be formed. 5 Using hPL to treat various ocular surface diseases, such as dry eye syndrome, has previously shown positive results with successful healing and reduced inflammation. 6 The current commercially available fibrin glues contain high concentrations of fibrinogen (40 mg/mL to up to 120 mg/mL) and mostly remains not transparent, making the component highly viscous and incompatible with functional visual acuity during healing.…”

Section: Introductionmentioning

confidence: 99%

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Development of an In Situ Printing System With Human Platelet Lysate-Based Bio-Adhesive to Treat Corneal Perforations

You

Frazer

Sayyar

et al. 2022

Trans. Vis. Sci. Tech.

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Purpose Corneal perforation is a clinical emergency that can result in blindness. Currently corneal perforations are treated either by cyanoacrylate glue which is toxic to corneal cells, or by using commercial fibrin glue for small perforations. Both methods use manual delivery which lead to uncontrolled application of the glues to the corneal surface. Therefore, there is a need to develop a safe and effective alternative to artificial adhesives. Methods Previously, our group developed a transparent human platelet lysate (hPL)-based biomaterial that accelerated corneal epithelial cells healing in vitro. This biomaterial was further characterized in this study using rheometry and adhesive test, and a two-component delivery system was developed for its application. An animal trial (5 New Zealand white rabbits) to compare impact of the biomaterial and cyanoacrylate glue (control group) on a 2 mm perforation was conducted to evaluate safety and efficacy. Results The hPL-based biomaterial showed higher adhesiveness compared to commercial fibrin glue. Treatment rabbits had lower pain scores and faster recovery, despite generating similar scar-forming structure compared to controls. No secondary corneal ulcer was generated in rabbits treated with the bio-adhesive. Conclusions This study reports an in situ printing system capable of delivering a hPL-based, transparent bio-adhesive and successfully treating small corneal perforations. The bio-adhesive-treated rabbits recovered faster and required no additional analgesia. Translational Relevance The developed in situ hPL bio-adhesives treatment represents a new format of treating corneal perforation that is easy to use, allows for accurate application, and can be a potentially effective and pain relief treatment.

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Section: Introductionsupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Development of an In Situ Printing System With Human Platelet Lysate-Based Bio-Adhesive to Treat Corneal Perforations

You

Frazer

Sayyar

et al. 2022

Trans. Vis. Sci. Tech.

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“…In this study, we successfully developed a novel in-situ bioink based printing system to treat small corneal perforations. The bioink used was an hPL-based fibrin biomaterial developed by our group [4]. In this study, we examined mechanical properties of bioink, developed a hand-held device for its delivery, and evaluated its in vivo effect.…”

Section: Discussionmentioning

confidence: 99%

“…Our group recently discovered that by combining human platelet lysate (hPL) with low concentrations of fibrinogen and thrombin, a transparent fibrin-based gel can be formed [4]. Using hPL to treat various ocular surface diseases such as dry eye syndrome has previously shown positive results [5].…”

Section: Introductionmentioning

confidence: 99%

“…Our earlier work demonstrated that the newly developed hPL and fibrin biomaterials possess suitable mechanical properties that are compatible with extrusion-based printing processes and support corneal epithelial cell growth [4]. To maximise transparency, the fibrinogen concentration was reduced to 2mg/mL, the physiological concentration in blood [4].…”

Section: Introductionmentioning

confidence: 99%

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Development of anin-situprinting system with human platelet lysate-based bio-adhesive to treat corneal perforation

You

Frazer

Sayyar

et al. 2021

Preprint

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Purpose: Corneal perforation is a clinical emergency. Tissue glue to seal the perforation, and supplementary topical medication represents existing standard treatment. Previously, our group developed a transparent bioink that showed good cell compatibility and accelerated corneal epithelial cells healing in-vitro. This study aims to develop a novel treatment method for corneal perforation using this bioink. Methods: Rheometry was used to measure bioink behaviour at room and corneal surface temperatures. Bioink adhesiveness to porcine skin and burst pressure limit were also measured. Based on rheological behaviour, a hand-held biopen was developed to extrude the bioink onto the cornea. An animal trial (5 New Zealand white rabbits) to compare bioink and cyanoacrylate glue (control group) impact on a 2mm perforation was conducted to evaluate safety and efficacy. Results: Bioink has higher adhesiveness compared to commercial fibrin glue and can withstand burst pressure approximately 6.4x higher than routine intraocular pressure. Bioink-treated rabbits had lower pain score and faster recovery, despite generating similar scar-forming structure after healing compared to controls. No secondary corneal ulcer was generated in rabbits treated with bioink. Conclusions: This study reports a novel in-situ printing system capable of delivering a transparent bioink to the cornea and successfully treating small corneal perforations. Bioink-treated rabbits recovered faster to completely healed perforation and required no additional analgesia. Both groups showed scarred corneal tissue after healing, however no infection and inflammation was observed 3 weeks. The delivery system was easy to use and may represent an alternative treatment for corneal perforation.

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Development of a new plasma rich in growth factors membrane with improved optical properties

Anitua

Muruzábal

Fuente

et al. 2023

Annals of Anatomy - Anatomischer Anzeiger

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Development of a Platelet Lysate–Based Printable, Transparent Biomaterial With Regenerative Potential for Epithelial Corneal Injuries

Cited by 7 publications

References 29 publications

Development of an In Situ Printing System With Human Platelet Lysate-Based Bio-Adhesive to Treat Corneal Perforations

Development of an In Situ Printing System With Human Platelet Lysate-Based Bio-Adhesive to Treat Corneal Perforations

Development of anin-situprinting system with human platelet lysate-based bio-adhesive to treat corneal perforation

Development of a new plasma rich in growth factors membrane with improved optical properties

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