Development of a Point-of-Care Platform for Plant Health Assessment: A Microfluidic Approach

Abstract

Azelaic Acid (AzA) is a signaling molecule that plays a role in plant immune response when these are infected with pathogens, such as Botryitis cinerea, making its early detection in the field critical in the monitoring and prevention of large spread infection in crops. In this work, a microfluidic platform for the rapid detection of AzA in grapes is presented. AzA detection is achieved via an enzyme-enabled colorimetric reaction performed in a microfluidic chip coupled to a thin-film silicon photodiode. The detection of nM concentrations of AzA in buffer was achieved and initial results for AzA measurements in artificially spiked grape juice show potential for detection in realistic sample conditions.