Development of a Porcine Delayed Wound-Healing Model and Its Use in Testing a Novel Cell-Based Therapy

Hadad, Ivan; Johnstone, Brian H.; Brabham, Jeffrey G.; Blanton, Matthew; Rogers, Pamela I.; Fellers, Cory; Solomon, James L.; Merfeld-Clauss, Stephanie; DesRosiers, Colleen; Dynlacht, Joseph R.; Coleman, John J.; March, Keith L.

doi:10.1016/j.ijrobp.2010.05.002

Cited by 46 publications

(41 citation statements)

References 39 publications

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“…Sie stellen zudem eine ethisch unkritische (exogene) Stammzellquelle dar und können unkompliziert im Rahmen von Fettabsaugungen gewonnen werden [14]. Adipogene Stammzellen scheinen lokalen Strahlenschäden der Haut durch eine Verbesserung der Angiogenese sowie durch Optimierung der Remodellingprozesse entgegenzuwirken [28,29].…”

Section: Rolle Adipogener Stammzellenunclassified

Potenzial adipogener Stammzellen bei radiogenen Wundheilungsstörungen

Haubner

Gassner

2015

HNO

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Section: Rolle Adipogener Stammzellenunclassified

Potenzial adipogener Stammzellen bei radiogenen Wundheilungsstörungen

Haubner

Gassner

2015

HNO

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“…The size of radiation fields used in the current study (2 cm × 2 cm) is smaller than those from previous studies [2,16]. The nadir in microvessel density was reached at 6 weeks after irradiation of 15 Gy.…”

Section: Discussionmentioning

confidence: 66%

Development of a Porcine Skin Injury Model and Characterization of Dose-dependent Response to High-dose Radiation

Kim

Lee

Choi

et al. 2012

International Journal of Radiation Oncology*Biology*Physics

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A porcine skin model was developed to characterize the dose-dependent response to high-dose radiation. The dorsal skin of a mini pig was divided into four paraspinal sections, with 11 small irradiation fields (2 cm × 2 cm) in each section, and a single fraction of 15, 30, 50 or 75 Gy was delivered to each section using a 6 MeV electron beam. A spectrophotometer measured gross skin changes, and a biopsy for each radiation dose was performed in the 1st, 2nd, 4th, 6th and 9th weeks for histology, immunostaining with anti-CD31, and western blotting with IL-6 and TGF-β1 to determine the degree of skin damage. After a 4-week latency period, erythema and dry desquamation, moist desquamation, and ulceration appeared at 4, 6 and 9 weeks, respectively. Gross skin toxicity was more pronounced, occurred early and continued to progress with irradiation >50 Gy, whereas complete healing was observed 12 weeks after 15 Gy. Spectrophotometry showed erythema indices rapidly increased during the first 4 weeks after irradiation. The number of eosinophils began rising sharply at 4 weeks and normalized after reaching peaks at 7-8 weeks. Microvessel density showed a biphasic pattern with a transient peak at 1 week, a nadir at 4-6 weeks, and maximum recovery at 9 weeks. Increase in the levels of IL-6 and TGF-β1 was detected soon after irradiation. Most of these parameters indicated complete healing of the skin 12 weeks after 15 Gy. Our porcine skin model provides an effective platform for studying high-dose radiation-induced skin injury, in particular histologic and molecular changes, during the early latency period.

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“…Currently, studies have been carried out to investigate the treatment efficiency of ASCs on radiation-induced skin injury, and the treatment efficiencies have been satisfactory to some extent (Akita et al, 2010a, b;Ebrahimian et al, 2009;Hadad et al, 2010;Rigotti et al, 2007). Unfortunately, few studies have been carried out to investigate the treatment efficiency of stem cell therapy on muscular injury induced by irradiation.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Potential of Adipose Stem Cells in Tissue Repair of Irradiated Skeletal Muscle in a Rabbit Model

Sun

et al. 2014

Cellular Reprogramming

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The repairing function and differentiation potency of adipose stem cells (ASCs) transplantation following skeletal muscle injury induced by radiotherapy are still not well defined. In this study, one side of the buttocks of 64 New Zealand white rabbits underwent irradiation and were randomly divided into an ASCs group [5×10(7) ASCs labeled with CM-Dil and suspended in 1 mL of phosphate-buffered saline (PBS), via intramuscular injection] and a PBS group (1 mL of PBS, via intramuascular injection). ASCs were isolated in New Zealand white rabbits in vitro, and migration of ASCs labeled with CM-Dil was observed after transplantation in vivo. A significant decrease of histological severity scoring was found in irradiated tissue obtained in the ASCs group compared with that in PBS group. Additionally, compensatory hyperplasia was noted after ASCs transplantation in the injured tissues. Moreover, ASCs could upregulate the expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) and promote the angiogenesis of the injured tissues. Interestingly, myofilament-like structures were identified in irradiated muscle cells after ASCs transplantation. We concluded that ASCs transplantation could repair the radiation-induced skeletal muscle injury. Its mechanism may be, at least partly, associated with the upregulation of VEGF and bFGF, angiogenesis, promoting the compensatory hyperplasia of muscle satellite cells, as well as the myogenic differentiation.

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Development of a Porcine Delayed Wound-Healing Model and Its Use in Testing a Novel Cell-Based Therapy

Cited by 46 publications

References 39 publications

Potenzial adipogener Stammzellen bei radiogenen Wundheilungsstörungen

Potenzial adipogener Stammzellen bei radiogenen Wundheilungsstörungen

Development of a Porcine Skin Injury Model and Characterization of Dose-dependent Response to High-dose Radiation

Therapeutic Potential of Adipose Stem Cells in Tissue Repair of Irradiated Skeletal Muscle in a Rabbit Model

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