Development of a Porcine Model of Post-Hepatectomy Liver Failure

Arkadopoulos, Nikolaos; Defterevos, Georgios; Nastos, Constantinos; Papalois, Αpostolos; Kalimeris, Konstantinos; Papoutsidakis, Nikolaos; Καμπούρογλου, Γεώργιος; Kypriotis, Dimosthenis; Pafiti, Agathi; Kostopanagiotou, Georgia; Smyrniotis, Vassilios

doi:10.1016/j.jss.2011.06.006

Cited by 38 publications

(33 citation statements)

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“…Furthermore, the pathway of IL-1 signaling, including the cytokine itself as well as prominent parts of the NF-kappa B pathway indicate the relevance of inflammatory processes in the liver after extended resection. Thus, major functional features of the liver were impaired, and we concluded that the method of extended liver resection as described by Arkadopoulos 28 , which we applied in our study, caused severe liver damage.…”

Section: Resultsmentioning

confidence: 55%

“…Surgery was performed according to Arkadopoulos 28 , with modifications. After premedication (ketamine 15 mg/kg BW, atropine 0.01 mg/kg BW, and midazolam 0.5 mg/kg BW), pigs received a continuous total venous anesthesia and analgesia (midazolam 1 mg/kg BW/h, sufentanil 0.5 μg/kg BW/h, ketamine 5–15 mg/kg BW/h, pancuronium bromide 80 μg/kg BW/h).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Mesenchymal stem cells correct haemodynamic dysfunction associated with liver injury after extended resection in a pig model

Tautenhahn

Brückner

Uder

et al. 2017

Sci Rep

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In patients, acute kidney injury (AKI) is often due to haemodynamic impairment associated with hepatic decompensation following extended liver surgery. Mesenchymal stem cells (MSCs) supported tissue protection in a variety of acute and chronic diseases, and might hence ameliorate AKI induced by extended liver resection. Here, 70% liver resection was performed in male pigs. MSCs were infused through a central venous catheter and haemodynamic parameters as well as markers of acute kidney damage were monitored under intensive care conditions for 24 h post-surgery. Cytokine profiles were established to anticipate the MSCs’ potential mode of action. After extended liver resection, hyperdynamic circulation, associated with hyponatraemia, hyperkalaemia, an increase in serum aldosterone and low urine production developed. These signs of hepatorenal dysfunction and haemodynamic impairment were corrected by MSC treatment. MSCs elevated PDGF levels in the serum, possibly contributing to circulatory homeostasis. Another 14 cytokines were increased in the kidney, most of which are known to support tissue regeneration. In conclusion, MSCs supported kidney and liver function after extended liver resection. They probably acted through paracrine mechanisms improving haemodynamics and tissue homeostasis. They might thus provide a promising strategy to prevent acute kidney injury in the context of post-surgery acute liver failure.

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Section: Resultsmentioning

confidence: 55%

Section: Methodsmentioning

confidence: 99%

Mesenchymal stem cells correct haemodynamic dysfunction associated with liver injury after extended resection in a pig model

Tautenhahn

Brückner

Uder

et al. 2017

Sci Rep

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“…Liver ischemia and reperfusion has been the topic of intense study during the last decades since it is implicated in many clinical scenarios, including hemorrhagic shock and resuscitation, trauma [1], liver resections [2], and liver transplantation [3]. …”

Section: Introductionmentioning

confidence: 99%

Global Consequences of Liver Ischemia/Reperfusion Injury

Nastos

Kalimeris

Papoutsidakis

et al. 2014

Oxidative Medicine and Cellular Longevity

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249

225

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Liver ischemia/reperfusion injury has been extensively studied during the last decades and has been implicated in the pathophysiology of many clinical entities following hepatic surgery and transplantation. Apart from its pivotal role in the pathogenesis of the organ's post reperfusion injury, it has also been proposed as an underlying mechanism responsible for the dysfunction and injury of other organs as well. It seems that liver ischemia and reperfusion represent an event with “global” consequences that influence the function of many remote organs including the lung, kidney, intestine, pancreas, adrenals, and myocardium among others. The molecular and clinical manifestation of these remote organs injury may lead to the multiple organ dysfunction syndrome, frequently encountered in these patients. Remote organ injury seems to be in part the result of the oxidative burst and the inflammatory response following reperfusion. The present paper aims to review the existing literature regarding the proposed mechanisms of remote organ injury after liver ischemia and reperfusion.

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“…The surgical protocol has been previously described [4]. Briefly, in the I/R group the liver was mobilized and a side-to-side portacaval anastomosis was performed.…”

Section: Methodsmentioning

confidence: 99%