Development of a portable low-cost real-time PCR system

Chong, K. S.; Beng, Gan Kok; Then, Sue Mian

doi:10.1109/icoras.2017.8308041

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…The size of the Peltier element used for heating was 80 mm x 40 mm x 4 mm figured in Figure 1. A geometry with the shape of a block was made for the PCR chamber having a size of 81 mm x 40 mm x 15 mm [15]. The chamber had an upper outer diameter of 10.7 mm, an inner diameter of 6.7 mm, a height of 10.5 mm and a radius of 1.5 mm.…”

Section: Geometrical Designmentioning

confidence: 99%

Heat Transfer Simulation of Various Material for Polymerase Chain Reaction Thermal Cycler

Lischer¹,

Putra²,

Sahlan³

et al. 2021

JMechE

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Medical diagnosis is the initial stage in identifying a person's condition, disease or injury from its signs and symptoms. The diagnostic method is carried out quantitatively by using a diagnostic kit which measures data such as blood pressure, heart rate frequency and blood cell concentration. These diagnostic kits are available in their respective capabilities and their activities require medical facilities and logistical readiness to function. Furthermore, Indonesia's geographical condition which consists of many islands and mountains causes uneven distribution of health facilities and laboratories in each region. Therefore, resulting in problems such as inadequate access and availability of these diagnostic kit in each region. Presently, one of the most widely used diagnostic methods is the Polymerase Chain Reaction (PCR) which allows the amplification of specific fragments from complex DNA. In PCR, only a small amount of DNA is needed to produce enough replication copies which were further analyzed by microscopic examination. This process begins with thermal cycling, which is the reactant's exposure to the heating cycle and repetitive repairs to produce reactions to different temperatures. This study aims to examine the material used for thermal cyclers which is an essential aspect of the heat transfer needed by the PCR process. In this study, heat transfer from several materials were simulated and analyzed by COMSOL Multiphysics 5.3 Software. The following results were obtained from the simulation: the saturation time for heating aluminum, copper and nickel were 29,37 and 51 seconds, respectively. Meanwhile, the cooling time was 26, 35 and 55 seconds, respectively. In addition, the saturation time for heating and cooling silver and Polydimethylsiloxane (PDMS) were 26 and 1480 seconds, respectively.

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Section: Geometrical Designmentioning

confidence: 99%

Heat Transfer Simulation of Various Material for Polymerase Chain Reaction Thermal Cycler

Lischer¹,

Putra²,

Sahlan³

et al. 2021

JMechE

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show abstract

“…How to realize a low-cost real-time fluorescence quantitative PCR system is a hot topic in the world [ 13 , 14 , 15 , 16 , 17 ]. For example, the researchers made a simple transformation of the traditional PCR to realize the quantitative PCR, which combines the traditional PCR instruments with smartphones and personal computers and designed an automatic fluorescence detection system with photoelectric feedback to achieve quantitative real-time PCR.…”

Section: Introductionmentioning

confidence: 99%

An Optimized Thermal Feedback Methodology for Accurate Temperature Control and High Amplification Efficiency during Fluorescent qPCR

et al. 2022

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Traditional qPCR instrument is combined with CMOS and a personal computer, and a photoelectric feedback automatic fluorescence detection system is designed to realize quantitative real-time PCR. The key to reaction efficiency lies in how to ensure that the temperature of the detection reagent completely matches the set temperature. However, for most traditional real-time fluorescent PCR systems, the temperature cycling is controlled by detecting the temperature of the heating well plate. It cannot directly measure the temperature in the reaction reagent PCR tube, which will cause the deviation in the actual temperature of the reagent to be as expected. Therefore, in this paper, we raise a method of directly detecting the temperature in the reaction tube of the reagent during the temperature cycling is adopted. According to the deviation from the expected value, the set temperature of the PCR instrument is adjusted to make the actual temperature of the reagent closer to the expected value. Through this method, we also realized the temperature calibration and optimization of the TEC circulation system we built. Experiments show that this low-cost, portable real-time quantitative PCR system can detect and analyze pathogens in situ.

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Combining FEM and GA for Small PCR Temperature Unit Thermal Cladding Thickness Optimization

Yang

Lei

et al. 2021

2021 China Automation Congress (CAC)

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Development of a portable low-cost real-time PCR system

Cited by 4 publications

References 12 publications

Heat Transfer Simulation of Various Material for Polymerase Chain Reaction Thermal Cycler

Heat Transfer Simulation of Various Material for Polymerase Chain Reaction Thermal Cycler

An Optimized Thermal Feedback Methodology for Accurate Temperature Control and High Amplification Efficiency during Fluorescent qPCR

Combining FEM and GA for Small PCR Temperature Unit Thermal Cladding Thickness Optimization

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