With the improvement of aircraft flight speed, bird impact is easy to cause serious accidents such as windshield breakdown, aircraft destruction and human death. In order to improve the bird impact resistance of aircraft windshield glass, the dynamic response of windshield glass (YB-3 plexiglass) after bird impact of small general-purpose aircraft flying at cruise speed was studied. By establishing the bird impact windshield glass model and adopting the fixed restraint mode around, the windshield glass is impacted at the speeds of 200km/h, 220km/h and 250km/h and the angles of 30°, 60° and 90°, respectively. The changes of stress-strain, displacement and energy absorption of the windshield glass under the impact of bird body, as well as the effects of different impact speeds and impact angles on the dynamic response of the glass are analyzed. It is found that during the whole impact process, the stress and strain of YB-3 plexiglass will reach the peak in a short time, then oscillate back and forth in a period of time, and the final residual deformation is small. With the increase of impact velocity, the stress, strain and displacement of glass will increase accordingly; With the increase of the impact angle, the energy absorbed by the windshield glass decreases accordingly, and the damage and deformation around the impact point are reduced. By studying the bird impact of the windshield of small general-purpose aircraft, it provides a theoretical basis for the airworthiness certification of general-purpose aircraft and the design optimization of windshield glass.