Development of a Pupal Color-Based Genetic Sexing Strain of the Melon Fly, &lt;I&gt;Bactrocera cucurbitae&lt;/I&gt; (Coquillett) (Diptera: Tephritidae)

McInnis, Donald O.; Tam, Steven Y. T.; Lim, Richard P.; Komatsu, J.; Kurashima, Rick; Albrecht, Christopher

doi:10.1603/0013-8746(2004)097[1026:doapcg]2.0.co;2

Cited by 80 publications

(72 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The 3R arms show limited similarity; only the tips can be correlated. ported by the construction of a genetic sexing strain based on a Y-autosome translocation (McInnis et al 2004). The exact structure of this translocation was also determined genetically and cytogenetically (unpublished data).…”

Section: Discussionmentioning

confidence: 99%

“…Fifteen morphological markers have been described and assigned to Þve autosomal linkage groups (McCombs et al 1996). Recently, a genetic sexing strain has been constructed based on a pupal color marker McInnis et al 2004). Genetic analyses of wild and natural populations have been reported using random ampliÞed polymorphic DNA markers (Haymer 1995) and mitochondrial DNA (Muraji and Nakahara 2001, Jamnongluk et al 2003, Zhu et al 2005, Hu et al 2008).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Mitotic and Polytene Chromosomes and Photographic Polytene Chromosome Maps inBactrocera cucurbitae(Diptera: Tephritidae)

Zacharopoulou

Sayed

Augustinos³

et al. 2011

Annals of the Entomological Society of America

View full text Add to dashboard Cite

We report here a cytogenetic analysis of the melon fly, Bactrocera Cucurbitae, Coquillett (Diptera: Tephritidae), a species of significant agricultural importance. The mitotic karyotype and detailed photographic maps of the larval salivary gland polytene chromosomes of the species are presented. The mitotic karyotype consists of six pairs of chromosomes including one pair of heteromorphic sex (XX/XY) chromosomes. The heterogametic sex is ascribed to the male. The analysis of polytene chromosomes has shown a total number of five long polytene elements (10 polytene arms) that correspond to the five autosomes. The characteristic features and the most prominent landmarks of each polytene chromosome are presented. The proposed chromosomal homology between B. Cucurbitae and Mediterranean fruit fly, Ceratitis capitata (Wiedemann), was determined by comparing chromosome banding patterns. The detection of heterozygous chromosome inversions in two strains is shown and discussed. The current study provides workable polytene chromosome maps of the species and compares our results with previous reports. We show that these maps can be used for cytogenetic studies in the species and for comparative studies among the tephritid species. They also can support the development of control methods and clarify the taxonomic status of B. cucurbitae.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Analysis of Mitotic and Polytene Chromosomes and Photographic Polytene Chromosome Maps inBactrocera cucurbitae(Diptera: Tephritidae)

Zacharopoulou

Sayed

Augustinos³

et al. 2011

Annals of the Entomological Society of America

View full text Add to dashboard Cite

show abstract

“…species that are the closest relative of each other and have not been distinguished from one another taxonomically (Bickford et al 2007). If this were true, the use of the genetic sexing strain developed at the (USDA-ARS)United States Department of Agriculture, Agricultural Research Services facilities in Hawaii by McInnis et al (2004) for sterile male releases in Mauritius would have been at risk. Mating compatibility studies between populations from Mauritius, Seychelles and the genetic sexing strain from Hawaii using the Mating Performance Field Cage Test showed, however, that the flies were fully compatible with no evidence for incipient speciation in spite of the differences in host use (Sookar et al 2013).…”

Section: Mating Compatibility Field Cage Tests In the Tephritidaementioning

confidence: 99%

Evaluating mating compatibility within fruit fly cryptic species complexes and the potential role of sex pheromones in pre-mating isolation

Juárez¹,

Devescovi²,

Břízová³

et al. 2015

View full text Add to dashboard Cite

The study of sexual behavior and the identification of the signals involved in mate recognition between con-specifics are key components that can shed some light, as part of an integrative taxonomic approach, in delimitating species within species complexes. In the Tephritidae family several species complexes have received particular attention as they include important agricultural pests such as the Ceratitis fasciventris (Bezzi), Ceratitis anonae (Graham) and Ceratitis rosa Karsch (FAR) complex, the Bactrocera dorsalis (Hendel) complex and the Anastrepha fraterculus (Wiedemann) complex. Here the value and usefulness of a methodology that uses walk-in field cages with host trees to assess, under semi-natural conditions, mating compatibility within these complexes is reviewed, and the same methodology to study the role of chemical communication in pre-mating isolation among Anastrepha fraterculus populations is used. Results showed that under the same experimental conditions it was possible to distinguish an entire range of different outcomes: from full mating compatibility among some populations to complete assortative mating among others. The effectiveness of the methodology in contributing to defining species limits was shown in two species complexes: Anastrepha fraterculus and Bactrocera dorsalis, and in the case of the latter the synonymization of several established species was published. We conclude that walk-in field cages constitute a powerful tool to measure mating compatibility, which is also useful to determine the role of chemical signals in species recognition. Overall, this experimental approach provides a good source of information about reproductive boundaries to delimit species. However, it needs to be applied as part of an integrative taxonomic approach that simultaneously assesses cytogenetic, molecular, physiological and morphological traits in order to reach more robust species delimitations.

show abstract

“…DISCUSIÓN Los datos de los atributos biológicos (fecundidad, fertilidad y supervivencia) para los estados inmaduros, indican que los insectos LFM poseen la misma capacidad que las moscas LFS y por lo tanto pueden ser criados bajo las mismas condiciones. Estas características han sido reportadas en especies como C. capitata (Rossler, 1979;Niyazi et al, 2005), A. fraterculus (Yamada & Selivon, 2001) y recientemente en Bactrocera cucurbitae (McInnis et al 2004, Harris et al 2006 donde se estudió ampliamente la biología y el comportamiento sexual. Trabajos similares también fueron realizados por Saul y McCombs (1992) con mutantes de C. capitata (Wiedemann), B. dorsalis (Hendel) y B. cucurbitae (Coquillet), ellos concluyeron que las mutantes de ojos we, we y le, poseen una capacidad reproductiva similar a sus homólogos tipo silvestre y permiten una rápida identificación en campo, que los coloca como candidatas a ser usadas como marcadores genéticos en programas de control.…”

Section: Resultsunclassified

Biología y comportamiento sexual del mutante ojos amarillos de Anastrepha ludens (Diptera: Tephritidae).

Luís

Fong

Salvador

et al. 2009

AZM

View full text Add to dashboard Cite

. 2009. Biología y comportamiento sexual del mutante ojos amarillos de Anastrepha ludens (Diptera: Tephritidae). Acta Zool. Mex. (n. s.) 25(1):9-20. RESUMEN. Se comparó la biología y el comportamiento sexual entre una cepa de laboratorio con fenotipo silvestre (LFS) con una cepa de laboratorio con fenotipo mutante (LFM) de Anastrepha ludens (Loew), que se caracteriza por una coloración del cuerpo clara y ojos amarillo cremoso, para determinar su potencial como marcador genético en los programas de control mediante el uso de la técnica del insecto estéril. Los resultados del estudio indicaron que los individuos de la cepa mutante LFM no difieren significativamente de los individuos de la cepa LFS respecto a su fecundidad, fertilidad y supervivencia de inmaduros, así como en la emisión de feromona sexual. Esto es indicativo de que podría ser adaptada a las condiciones de cría masiva. Sin embargo, las pruebas de apareamiento indicaron que aunque los insectos LFM copularon con los insectos de campo de fenotipo silvestre (CFS), presentaron cierto grado de aislamiento y bajos porcentajes de apareamiento de los machos tanto con hembras CFS y LFS, lo cual implica que no sea recomendable el uso de la cepa mutante LFM como marcador genético en programas que utilizan la técnica del insecto estéril, para el control de la mosca Mexicana de la fruta. Palabras clave: Anastrepha ludens, comportamiento sexual, cría masiva, mutante, marcador genético. ABSTRACT. We compared the biology and sexual behavior between a strain of laboratory with wild phenotype (LFS) and a strain of laboratory with mutant phenotype (LFM) of Anastrepha ludens (Loew), characterized by clear coloration of the body and creamy yellow eyes to determine their potential as genetic marker in the control programmes through the use of the sterile insect technique.

show abstract

Development of a Pupal Color-Based Genetic Sexing Strain of the Melon Fly, <I>Bactrocera cucurbitae</I> (Coquillett) (Diptera: Tephritidae)

Cited by 80 publications

References 16 publications

Analysis of Mitotic and Polytene Chromosomes and Photographic Polytene Chromosome Maps inBactrocera cucurbitae(Diptera: Tephritidae)

Analysis of Mitotic and Polytene Chromosomes and Photographic Polytene Chromosome Maps inBactrocera cucurbitae(Diptera: Tephritidae)

Evaluating mating compatibility within fruit fly cryptic species complexes and the potential role of sex pheromones in pre-mating isolation

Biología y comportamiento sexual del mutante ojos amarillos de Anastrepha ludens (Diptera: Tephritidae).

Contact Info

Product

Resources

About