Development of a quality monitoring program for platelet components: a report of the first four years' experience at Canadian Blood Services

Levin, E.J.; Jenkins, Craig; Culibrk, Brankica; Gyöngyössy‐Issa, Maria I.C.; Serrano, Katherine; Devine, Dana V.

doi:10.1111/j.1537-2995.2011.03402.x

Cited by 20 publications

(24 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using previous QMP data [11], a noninferiority margin of 7% was determined for CD62P expression (Table 1). Both on Day 1 and at expiry, no significant difference in CD62P expression between M and NM platelets was observed (Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…Assessment of platelet degranulation, a marker of platelet activation, was conducted using CD62P expression by flow cytometry on a FACS Canto II (BD, Mississauga, Ontario, Canada), as described previously [1, 11]. Platelet morphology was assessed by modified Kunicki scoring, as described previously [1, 11, 12].…”

Section: Methodsmentioning

confidence: 99%

“…Platelet morphology was assessed by modified Kunicki scoring, as described previously [1, 11, 12]. ESC was measured using a SPA-2000 (Chronolog Corp., Havertown, PA) according to Holme et al [13] and described in detail previously [1].…”

Section: Methodsmentioning

confidence: 99%

“…Some tests were conducted both early and late in storage to determine whether quality was affected immediately after processing. Secondly, all QC parameters were assessed to determine whether components produced without mixing were noninferior to those produced using the standard protocol, based on noninferiority/equivalence margins, determined by reviewing one year of CBS QC data and data from an on-going internal Quality Monitoring Program (QMP) [11, 15]. Additional quality parameters were also measured, and if there were sufficient prior QMP data (CD62P expression for platelets and supernatant K + for RBCs), noninferiority margins were determined and applied (Table 1).…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Process Improvement by Eliminating Mixing of Whole Blood Units after an Overnight Hold Prior to Component Production Using the Buffy Coat Method

Mastronardi

Schubert

Levin

et al. 2013

Journal of Blood Transfusion

Self Cite

View full text Add to dashboard Cite

The elimination of a thorough manual mixing of whole blood (WB) which takes place following the overnight hold, but before the first centrifugation step, during buffy coat component production at Canadian Blood Services (CBS) was investigated. WB was pooled after donation and split. Pairs of platelet, red blood cell (RBC), and plasma components were produced, with half using the standard method and half using a method in which the mixing step was eliminated. Quality assessments included yield, pH, CD62P expression and morphology for platelets, hemoglobin, hematocrit, hemolysis, and supernatant K+ for RBCs, and volume and factor VIII activity levels for plasma. All components, produced using either method, met CBS quality control criteria. There were no significant differences in platelet yield between components produced with and without mixing. A significant difference was seen for RBC hemolysis at expiry (P = 0.03), but for both groups, levels met quality control requirements. Noninferiority of components produced without mixing was confirmed for all parameters. Manual mixing is laborious and has a risk of repetitive strain for production staff and its significance is unclear. Elimination of this step will improve process efficiencies without compromising quality.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Process Improvement by Eliminating Mixing of Whole Blood Units after an Overnight Hold Prior to Component Production Using the Buffy Coat Method

Mastronardi

Schubert

Levin

et al. 2013

Journal of Blood Transfusion

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is partly based on fact that dynamicity of platelet and loss of CD62P surface protein. 21 There was evidence that P-selectin level increased during storage, whereas its activatability undergo reduction.…”

mentioning

confidence: 99%

Comparing P-Selectin (CD62P) expression in patients receiving non-leukodepleted vs leukodepleted thrombocyte concentrates

et al. 2017

View full text Add to dashboard Cite

Thrombocyte concentrate (TC) transfusion is an important supportive therapy in patients with thrombocytopenia. The risks in platelet transfusions may be related to the content of TC including the contaminant leukocytes. The aim of this study was to assess the risk of increased level of P-Selectin (CD62P) expression of non-leukodepleted TC transfusions. This was a quasi-experimental study. Subjects were children patients aged 1-18 years who received a non-leukodepleted or a leukodepleted TC transfusions. Comparison of the proportion of increased expression of CD62P in both groups expressed as relative risk. The subjects consisted of 51 patients who received non-leukodepleted and 52 patients who received leukodepleted TC transfusions. The risk of increased expression of CD62P in patients receiving non-leukodepleted TC transfusions were 2.38 (95%CI:1.60-3.53) times higher than those who received leukodepleted TC. Non-leukodepleted have significant higher risks of increased CD62P expression than leukodepleted TC transfusions. ABSTRAKTransfusi thrombocyte concentrate (TC) merupakan terapi pendukung yang penting pada pasien yang mengalami trombositopenia. Risiko yang terjadi dapat terkait dengan komponen yang terkandung dalam TC termasuk adanya lekosit kontaminan. Penelitian ini bertujuan untuk menilai risiko berupa kejadian peningkatan ekspresi P-selectin (CD62P) pada transfusi TC non-lekodeplesi. Penelitian ini merupakan penelitian eksperimental semu. Subjek penelitian adalah pasien anak usia 1-18 tahun yang mendapat transfusi TC non-lekodeplesi dan lekodeplesi. Perbandingan proporsi peningkatan ekspresi CD62P pada kedua kelompok dinyatakan dalam risiko relatif. Subjek terdiri dari 51 pasien yang mendapatkan transfusi TC non-lekodeplesi dan 52 pasien yang mendapatkan transfusi TC lekodeplesi. Risiko peningkatan ekspresi CD62P pada pasien yang mendapatkan transfusi TC non-lekodeplesi adalah 2,38 (95%CI:1,60-3,53) kali lebih tinggi dibandingkan yang mendapatkan transfusi TC lekodeplesi. Transfusi TC non-lekodeplesi memiliki risiko yang lebih tinggi bermakna dibandingkan TC lekodeplesi.

show abstract

Platelet‐rich plasma‐loaded chitosan scaffolds: Preparation and growth factor release kinetics

et al. 2012

View full text Add to dashboard Cite

The aim of this study is to compare the effects of different platelet-rich plasma (PRP) preparation methods on platelet activity and to investigate the growth factor (GF) release kinetics from PRP-loaded chitosan scaffolds for tissue engineering applications. Flow cytometry analysis showed that centrifugation processes used for PRP preparation did not cause significant effect on platelet activation levels by means of markers investigated. Two different methods were used to prepare PRP-loaded chitosan scaffolds: (i) PRP was added to chitosan gel before freeze-drying to prepare scaffolds called as "GEL" and (ii) PRP was embedded to freeze-dried chitosan scaffolds to prepare scaffolds called as "SPONGE." In addition, nonactivated PRP and PRP activated with type-I collagen were used as control groups. Scanning electron microscopy images demonstrated that, in GEL group, there is no deterioration on the scaffolds porous, 3D, and interconnected structure. GF release kinetics was determined by enzyme-linked immunosorbent assay for platelet-derived GF-BB, transforming GF-β1, and insulin-like GF-1. A sustained release of GFs was achieved in GEL group while a sharp burst release was observed for all the GFs from the SPONGE groups. Moreover, platelet-derived GF-BB, insulin-like GF-1, and transforming GF-β1 releases were prolonged to 20 days in GEL groups, and the biological activities of all GFs released from GEL and SPONGE scaffolds were preserved. This study demonstrated that chitosan scaffold that was called GEL could be an appropriate carrier for PRP applications by providing sustained release of GFs.

show abstract

Development of a quality monitoring program for platelet components: a report of the first four years' experience at Canadian Blood Services

Abstract: A QMP was found to be useful to monitor production processes across sites and highlights best practice approaches while deepening understanding of the quality of PLT products at CBS.

Cited by 20 publications

References 28 publications

Process Improvement by Eliminating Mixing of Whole Blood Units after an Overnight Hold Prior to Component Production Using the Buffy Coat Method

Process Improvement by Eliminating Mixing of Whole Blood Units after an Overnight Hold Prior to Component Production Using the Buffy Coat Method

Comparing P-Selectin (CD62P) expression in patients receiving non-leukodepleted vs leukodepleted thrombocyte concentrates

Platelet‐rich plasma‐loaded chitosan scaffolds: Preparation and growth factor release kinetics

Contact Info

Product

Resources

About