Development of a quantification system of ionic dissociative metabolites using an FT-IR/ATR method

Pan, Tao; Hashimoto, Atsushi; Kanou, Mikihito; Nakanishi, Kenichi; Kameoka, Takaharu

doi:10.1007/s00449-003-0343-z

Cited by 33 publications

(28 citation statements)

References 13 publications

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“…In this case, the sugar content determined by the MIR spectroscopic method when the presence of the ethanol in the medium was also taken into account, indicated a better accuracy than that determined by our previous method which neglected the absorption peaks of the ethanol in the medium [20]. Therefore, the spectroscopic method outlined in this paper could be used to analyze the overall kinetics of the metabolism of the suspension plant cells, because the FT-IR/ATR method has the potential to theoretically determine most of the metabolites, including ionic dissociative materials [29,30]. This study represents a very important step in such developments.…”

Section: Resultsmentioning

confidence: 92%

Simple and rapid determination of metabolite content in plant cell culture medium using an FT-IR/ATR method

Hashimoto

Yamanaka

Kanou

et al. 2004

Bioprocess Biosyst Eng

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A simple, rapid and accurate mid-infrared (MIR) spectroscopic method for simultaneously determining the product (ethanol) content and the nutrient (sugar) content in plant-cell culture media was developed using a Fourier transform infrared (FT-IR) spectrometer equipped with an attenuated total reflectance (ATR) accessory. We assessed the potential of this method by comparing it to a high-performance liquid chromatography (HPLC) method, and using the developed method to measure the ethanol and sugar contents simultaneously in liquid culture media with rice and tabacum cell suspensions, respectively. The experimental results suggest that the sugar consumption and ethanol production behaviors of the plant cell suspensions can be non-destructively and simultaneously monitored using the developed method. Furthermore, the spectroscopic method provided in this study could be developed into a technique that could be used to analyze the overall kinetics of the metabolism of the plant cell suspensions.

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Section: Resultsmentioning

confidence: 92%

Simple and rapid determination of metabolite content in plant cell culture medium using an FT-IR/ATR method

Hashimoto

Yamanaka

Kanou

et al. 2004

Bioprocess Biosyst Eng

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“…This bu®er is suitable for use in simulating the physiological environment of a living body, such as the enzyme reaction in cell sap. 21,22 In this study, the Tris-HCl bu®er was used to simulate the background liquid of TCP. A quantitative analysis of a mixture of TCP with the TrisHCl bu®er (denoted as the TCP-Tris-HCl mixture) was performed to explore the feasibility of the quantitative analysis of TCP in complex systems (such as fermentation broth or blood).…”

Section: Introductionmentioning

confidence: 99%

AO–MW–PLS method applied to rapid quantification of teicoplanin with near-infrared spectroscopy

Chen

Pan

et al. 2017

J. Innov. Opt. Health Sci.

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Teicoplanin (TCP) is an important lipoglycopeptide antibiotic produced by fermenting Actinoplanes teichomyceticus. The change in TCP concentration is important to measure in the fermentation process. In this study, a reagent-free and rapid quanti¯cation method for TCP in the TCP-Tris-HCl mixture samples was developed using near-infrared (NIR) spectroscopy by focusing our attention on the fermentation process for TCP. The absorbance optimization (AO) partial least squares (PLS) was proposed and integrated with the moving window (MW) PLS, which is called AO-MW-PLS method, to select appropriate wavebands. A model set that includes various wavebands that were equivalent to the optimal AO-MW-PLS waveband was proposed based on statistical considerations. The public region of all equivalent wavebands was just one of the equivalent wavebands. The obtained public regions were 1540-1868 nm for TCP and 1114-1310 nm for Tris. The root-mean-square error and correlation coe±cient for leave-oneout cross validation were 0.046 mg mL À1 and 0.9998 mg mL À1 for TCP, and 0.235 mg mL À1 and 0.9986 mg mL À1 for Tris, respectively. All the models achieved highly accurate prediction e®ects, and the selected wavebands provided valuable references for designing specialized spectrometers. This study provided a valuable reference for further application of the proposed methods to TCP fermentation broth and to other spectroscopic analysis¯elds.

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“…) % ( 100 RMSEP RRMSEP × = mp C (6) where C' ip , C ip are predictive value and chemical values of i sample in the prediction set, C' mp , C mp are the mean predicted value and mean chemical value of all samples in the prediction set, M is the sample number in the prediction set. RMSEP was used as the goal of model optimization and parameter design.…”

Section: Model Evaluation Indicatorsmentioning

confidence: 99%

“…And they could be used the quantitative analysis of online, real-time and in situ which no chemical reagents. The FTIR/ATR spectroscopy technique had been extensive applied to the analysis of agricultural product and food [2] , fermentation process monitoring [3] , enzyme activity analysis [4] , cell metabolism measurement [5][6] and many other fields.…”

Section: Introductionmentioning

confidence: 99%

Wavenumbers Selection for FTIR/ATR Spectroscopy Analysis of Hemoglobin in Human Whole Blood

Yin

Pan

Xie

et al. 2010

2010 4th International Conference on Bioinformatics and Biomedical Engineering

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The directly rapid determination method and the optimal analysis model of hemoglobin (HGB) in human whole blood were established by using the Fourier transform infrared spectroscopy (FTIR) and attenuated total reflection (ATR) technology. Based on the prediction effect of single wavenumber linear regression model, a wavenumbers selection method of FTIR/ATR spectroscopy analysis was developed. 20 single wavenumber models which good prediction effect and the corresponding wavenumbers were selected. All wavenumber combinations of the twenty wavenumbers were used to establish multiple linear regression (MLR) models respectively. The optimal model was selected according to the prediction effect. The optimal MLR model was the eight wavenumbers combination of 2860, (cm -1 ), the corresponding root mean squared error of predication (RMSEP), correlation coefficient of predication (R P ) and relative root mean squared error of predication (RRMSEP) were 6.63 g/L, 0.827, and 4.92% respectively. And the prediction effect was obvious better than one of partial least squares (PLS) model. These indicated that the wavenumbers selection method based on prediction effect of single wavenumber linear regression model could be applied to the FTIR/ATR spectroscopy analysis of hemoglobin in human whole blood.

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Development of a quantification system of ionic dissociative metabolites using an FT-IR/ATR method

Cited by 33 publications

References 13 publications

Simple and rapid determination of metabolite content in plant cell culture medium using an FT-IR/ATR method

Simple and rapid determination of metabolite content in plant cell culture medium using an FT-IR/ATR method

AO–MW–PLS method applied to rapid quantification of teicoplanin with near-infrared spectroscopy

Wavenumbers Selection for FTIR/ATR Spectroscopy Analysis of Hemoglobin in Human Whole Blood

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