Development of a quantitative approach using Raman spectroscopy for carotenoids determination in processed sweet potato

Sebben, Juliano Antônio; Espíndola, Juliana da Silveira; Ranzan, Lucas; Moura, Neusa Fernandes de; Trierweiler, Jorge O.; Trierweiler, Jorge Otávio

doi:10.1016/j.foodchem.2017.11.086

Cited by 32 publications

(18 citation statements)

References 17 publications

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“…However, mapping can become a powerful tool when combined with spectral filters for Raman peaks and threshold functions to select different subset of the data, which can be more reproducible and comparable between samples . In the case of carotenoids, the intensity of their Raman peaks, especially the 1,515 cm −1 one corresponding to the CC in‐phase stretching vibrations, has been successfully correlated to intracellular carotenoid concentration and thus used to monitor their degradation to different treatments including UV irradiation, gamma irradiation, thermic treatments, or nitrogen stress . We thus treated the data in SNR and compared only the maximal signals by creating masks (as depicted in Figure ) to quantitatively ascertain the persistence of the carotenoid signal.…”

Section: Resultsmentioning

confidence: 99%

“…But the other peaks show values above 90% of control values, independently of the applied dose (Figure c). The height of the three carotenoids' peaks has been shown to scale linearly with carotenoid concentration, but the best correlation was achieved with the 1,515 cm −1 peak . The differences in fluorescent backgrounds could disturb this correlation; the P‐MRS analogue mixture is known to produce more fluorescence than the S‐MRS analogue when analyzed with Raman spectroscopy using a 532‐nm excitation wavelength .…”

Section: Resultsmentioning

confidence: 99%

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Protection of cyanobacterial carotenoids' Raman signatures by Martian mineral analogues after high‐dose gamma irradiation

Baqué

Hanke

Böttger

et al. 2018

J Raman Spectroscopy

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The future robotic exploration missions to Mars-European Space Agency/ Roscosmos's ExoMars2020 and National Aeronautics and Space Administration's Mars2020 rovers-will search for signs of extant or extinct life using, among other instruments, Raman spectrometers for the first time. The question remains whether organic biosignatures-such as pigments and cellular components-may be detected by this method. Evaluating their detection limit under simulated extraterrestrial conditions is therefore crucial for the success of future life detection missions. Ionizing radiation can be considered as the most deleterious factor for the long-term preservation of potential biomarkers on Mars. Here, we report on the preservation potential of Raman signatures in the Antarctic strain CCCryo 231-06 of the cyanobacterium Nostoc sp.after high doses of gamma irradiation. The carotenoids' signals, a wellestablished biosignature model, dominate the Raman spectra at 532-nm excitation wavelength due to resonance effects. But comparing their distribu-KEYWORDS biosignature, carotenoids, ionizing radiation, Raman spectroscopy, search for life on MarsThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Protection of cyanobacterial carotenoids' Raman signatures by Martian mineral analogues after high‐dose gamma irradiation

Baqué

Hanke

Böttger

et al. 2018

J Raman Spectroscopy

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“…On the contrary, the drying using an oven with hot‐air led to higher losses due to the prolonged exposure to the air, and at temperatures more favorable to the oxidation reaction, when compared to freeze‐drying. According to Sebben et al (2018), reduction of around fifty percent of carotenoid content in orange‐fleshed sweet potato was obtained after 125 min under hot‐air exposure, and also, by 8 min using microwave drying. In the present work, the carotenoids contents, that is, the sum of β‐carotene and lycopene, shows a reduction correspondent at around 16% and 43% for the microwave (15 min) and hot‐air (200 min), respectively.…”

Section: Resultsmentioning

confidence: 99%

Study of three drying methods in production of nutritious flours from the fermentation slurry of orange‐fleshed sweet potato

Schweinberger

Sebben

Schultz

et al. 2020

J Food Process Preserv

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When producing ethanol from sweet potatoes, the fermentation generates slurry with nutritional content but underutilized for human consumption. Therefore, the nutritional properties of the flours obtained from the fermentation slurry of orange‐fleshed sweet potato (rich in carotenoids) was investigated. Three drying methods were carried out to produce slurry flours: convective using a hot‐air (60°C), microwave, and lyophilization. For carotenoids determination, were applied a conventional method and the Raman spectroscopy, a modern and alternative technique. Regarding the drying methods, the microwave was the most promising since it produced flour presenting higher levels of carotenoids (β‐carotene 849.7 µg/g and lycopene 548.4 µg/g). Also, the microwave drying time was 15 min, being much faster than the other methods. In the centesimal analysis of macronutrients, the slurry flours presented high fiber content, around 55 g/100 g. Finally, biscuits containing parcels of the flours demonstrated potential acceptability, by sensory analysis evaluating aroma and appearance. Practical applications Since ethanol is a product of worldwide importance, and it is produced mainly from corn and sugar cane, the production from sweet potatoes as an alternative raw material has interested researchers from different countries. Knowing that the ethanol production from sweet potato generates a residual slurry, this study focuses on adding value to this slurry, producing a nutritious flour. The potential for industrial application is promising, assuming it is a residue of an industrial infrastructure already installed. For the industry, obtaining more than one product in the same process represents a financial advantage and resource optimization.

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“…The obtained spectral information contains not only the chemical structure information of the sample, but also many background and noise signals from the interference source such as the instrument itself and the experimental operating environment. Therefore, in order to eliminate the influence of the extraneous and interfering signals on the sample signal, the original data can be preprocessed [21]. In this study, five data preprocessing methods were applied including multiplicative scatter correction (MSC), wavelet transform (WT), standard normal variate (SNV), rolling-circle filter (RCF) and adaptive iteratively reweighted penalized least squares (airPLS).…”

Section: Spectral Pretreatmentmentioning

confidence: 99%

Quantitative visualization of photosynthetic pigments in tea leaves based on Raman spectroscopy and calibration model transfer

Zeng

Zhang

Luo

et al. 2020

Preprint

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Background: Photosynthetic pigments participating in the absorption, transformation and transfer of light energy play a very important role in plant growth. While, the spatial distribution of foliar pigments is an important indicator of environmental stress, such as pests, diseases and heavy metal stress. Results: In this paper, in situ quantitative visualization of chlorophyll and carotenoid was realized by combining the Raman spectroscopy with calibration model transfer, and a laboratory Raman spectral model was successfully extended to a portable field spectral measurement. Firstly, a nondestructive and fast model for determination of chlorophyll and carotenoid in tea leaf was established based on confocal micro-Raman spectrometer in the laboratory. Then the spectral model was extended to a real-time foliar map scanning spectra of a field portable Raman spectrometer through calibration model transfer, and the spectral variation between the confocal micro-Raman spectrometer in the laboratory and the portable Raman spectrometer were effectively corrected by the direct standardization (DS) algorithm. The portable map scanning Raman spectra of the tea leaves after the model transfer were got into the established quantitative determination model to predict the concentration of photosynthetic pigments at each pixel of the tea leaves. The predicted photosynthetic pigments concentration of each pixel was imaged to illustrate the distribution map of foliar pigments. Statistical analysis showed that the predicted pigment contents were highly correlated with the real contents. Conclusions: It can be concluded that the Raman spectroscopy was applicable for in situ, non-destructive and rapid quantitative detecting and imaging of photosynthetic pigment concentration in tea leaves, and the spectral detection model established based on the laboratory Raman spectrometer can be applied to a portable field spectrometer for quantitatively imaging of the foliar pigments.

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Development of a quantitative approach using Raman spectroscopy for carotenoids determination in processed sweet potato

Cited by 32 publications

References 17 publications

Protection of cyanobacterial carotenoids' Raman signatures by Martian mineral analogues after high‐dose gamma irradiation

Protection of cyanobacterial carotenoids' Raman signatures by Martian mineral analogues after high‐dose gamma irradiation

Study of three drying methods in production of nutritious flours from the fermentation slurry of orange‐fleshed sweet potato

Quantitative visualization of photosynthetic pigments in tea leaves based on Raman spectroscopy and calibration model transfer

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